膜分离技术改善吐温80澄明度

发布时间：2018-03-11 00:10

本文选题：吐温　切入点：澄明度　出处：《中成药》2017年11期 　论文类型：期刊论文

【摘要】：目的采用膜分离技术改善吐温80澄明度,并探索影响澄明度的原因。方法选择10、50、100 k Da截留分子量的超滤膜分离吐温80溶液,以吐温80透过率、超滤和灭菌前后粒径分布及澄明度为指标,进行相关性分析。结果吐温80在10、50、100 k Da超滤膜中的透过率在24.4%~71.6%之间,与截留分子量呈正相关。10、50 k Da超滤膜处理后,澄明度均高于95%。超滤后,吐温80粒径分布范围明显减小,而且可以去除粒径大于1μm的微粒,低粒径分布的胶束在100 nm以下,高粒径分布的胶束在100~1 000 nm范围内。截留相对分子质量300、800、1 000 Da的纳滤膜对吐温80的截留率均大于90%,但1 000 Da纳滤膜损失相对较明显。结论粒径在1μm以上的高分子胶束及不溶性微粒是影响吐温80澄明度的主要因素。50 k Da超滤膜和800 Da纳滤膜联合使用时,不仅在保障澄明度的同时提高生产效率,而且纳滤浓缩可以满足制剂生产中对吐温80的浓度需求。
[Abstract]:Objective to improve the clarity of Tween 80 by membrane separation technique and to explore the causes of its influence. Methods the Tween 80 solution was separated by ultrafiltration membrane with 1050 ~ 100kDa molecular weight interception and the transmittance of Tween 80 was determined. Results the transmittance of Tween80 in 1050 ~ 100kDa ultrafiltration membrane was in the range of 24.4% and 71.6%, which was positively related to the molecular weight of interception. After ultrafiltration, the particle size distribution range of Twain 80 was obviously reduced, and the particle size larger than 1 渭 m could be removed. The micelle with low particle size distribution was below 100 nm. The micelles with high particle size distribution were in the range of 100 ~ 1 000 nm. The rejection rates of the nanofiltration membranes with relative molecular mass of 300 ~ 800 ~ 1 000 Da on Tween 80 were higher than 90%, but the loss of 1 000 Da nanofiltration membrane was relatively obvious. Conclusion Polymer with particle size above 1 渭 m has a larger loss. Micelles and insoluble particles were the main factors affecting Tween 80 clarity. 50 kDa ultrafiltration membrane and 800 Da nanofiltration membrane were used together. It can not only guarantee the clarity but also improve the production efficiency, and the concentration of Tween 80 can be satisfied by nanofiltration concentration.
【作者单位】：南京中医药大学药学院;南京中医药大学江苏省中药资源产业化过程协同创新中心;
【基金】：国家自然科学基金(81603307,81503258) 江苏省自然科学基金青年基金(BK20151005) 江苏省高校自然科学基金(17KJB360010) 南京中医药大学2016年大学生创新创业计划省级重点项目(2016)
【分类号】：TQ028.8;TQ461

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