微球三维位置快速精密测量的新方法

发布时间：2018-03-14 01:00

本文选题：测量　切入点：微球三维位置　出处：《光学学报》2017年01期 　论文类型：期刊论文

【摘要】：为了实现对液态环境中微球三维位置的快速精密测量,尤其是轴向的测量分辨力和速度,基于离焦成像测量理论,结合并改进象限插值法和矢径投影算法,提出了一种新的方法,实现了对微球三维方向1nm测量分辨力的跟踪测量。该方法测量效率高,对单个粒子轴向位置的测量速率最快达到每秒上百帧甚至更高。进一步讨论了横向位置测量误差对轴向测量分辨力的影响,分析了轴向方向大范围的跟踪测量分辨力。通过和相同实验条件下的互相关方法对比,说明该方法在实际测量中具有可行性,在实现相同轴向测量分辨力的情况下测量效率更高。
[Abstract]:In order to realize the fast and precise measurement of the three-dimensional position of microspheres in liquid environment, especially the axial measurement resolution and velocity, based on the defocus imaging measurement theory, the quadrant interpolation method and the vector projection algorithm are combined and improved. In this paper, a new method for tracking and measuring the resolution of microspheres at 1nm in 3D direction is proposed, which has high measurement efficiency. The axial position of a single particle can be measured at the fastest rate of hundreds of frames per second or higher. The influence of the lateral position measurement error on the resolution of the axial measurement is further discussed. The resolution of a wide range of tracking measurements in the axial direction is analyzed. By comparing with the cross-correlation method under the same experimental conditions, it is shown that the method is feasible in practical measurement. The measurement efficiency is higher when the resolution of the same axial measurement is achieved.
【作者单位】：天津大学精密仪器与光电子工程学院;英属哥伦比亚大学化学系;
【基金】：国家自然科学基金(51375340,61223008) 天津市自然科学基金(15JCZDJC31600)
【分类号】：TB921

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