3种石生苔藓植物碳酸酐酶对石灰岩的溶蚀作用

发布时间：2018-03-25 21:10

本文选题：石生苔藓　切入点：碳酸酐酶　出处：《中国岩溶》2017年04期

【摘要】：以普定严重石漠化区域石生细叶小羽藓Haplocladium microphyllum Hedw.、银叶真藓Bryum argenteum Hedw.和美灰藓Eurohypnum leptothollum(C.Muell.)Ando.为研究材料,运用模拟土柱实验装置收集植物淋出液并测定其电导率、pH值、CA活性、钙镁元素含量并对石灰岩试片溶蚀进行估算。结果表明:岩溶生态系统中不同植物的碳酸酐酶活性差异较大,其中细叶小羽藓的溶蚀效果最为显著,产生的碳汇量最大;CA活性最高为美灰藓,为272.99U·g~(-1)·FW;最小的为细叶小羽藓的CA活性较低,为38.31U·g~(-1)·FW。淋出液均检测到CA的存在,银叶真藓淋溶6d的CA活性最高,随着时间的推移,3种植物CA总体表现出先略降,然后升高,最后趋于平稳的趋势;pH值呈上升趋势;电导率呈下降趋势;淋出液中Ca~(2+)和Mg~(2+)浓度随淋溶时间的延长浓度逐渐降低,一定时间后趋于动态的平衡;说明石生植物对石灰岩有明显的酶促溶蚀驱动作用,且溶蚀率与CA活性成正相关。
[Abstract]:Using Haplocladium microphyllum Hedw., Bryum argenteum Hedw. and Eurohypnum leptothollumus C.Muell.Ando.Ando. in the severe rocky desertification area of Puding, the plant leachate was collected by simulated soil column experiment device and its conductivity and pH value CA activity were measured. The content of calcium and magnesium elements and the dissolution of limestone specimen were estimated. The results showed that the carbonic anhydrase activity of different plants in karst ecosystem varied greatly, among which the dissolution effect was the most obvious. The maximum amount of carbon sequestration produced was moss (272.99U / g) FW, and the lowest (38.31U / g ~ (-1)) FW.The CA activity was the highest in the elution solution, and the CA activity was the highest at 6 days after leaching of Moss silverleaf, while the activity of CA was the highest in the leached moss for 6 days, and the CA activity was lower (38.31U / g ~ (-1)) in the leachate. With the passage of time, CA of the three plants showed a slight decrease first, then increased, and finally tended to a steady trend: the pH value showed an upward trend, the electrical conductivity showed a downward trend; The concentration of Ca~(2 and Mg~(2 in leaching solution decreased gradually with the prolonging of leaching time and tended to dynamic equilibrium after a certain time, which indicated that lithophyte had obvious enzymatic dissolution driving effect on limestone, and the dissolution rate was positively correlated with CA activity.
【作者单位】：西南大学生命科学学院/三峡库区生态环境教育部重点实验室;安顺学院;
【基金】：国家自然科学基金(41463006)
【分类号】：P642.25;Q948.11

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