西芹根物质及种子六次酮层物化感物质分离鉴定及其对黄瓜枯萎病诱导抗性的研究

发布时间：2018-01-31 14:55

本文关键词： 西芹六次酮层物黄瓜枯萎病菌化感作用化感物质诱导抗性　出处：《内蒙古农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本试验以西芹根物质(即西芹鲜根、根际区物)及西芹种子为材料,利用柱层析法研究了六次酮层物流分对黄瓜枯萎病菌的化感作用,并且通过GC-MS对第六次层析得到的化感作用最佳的流分进行物质鉴定。结果表明:1.西芹鲜根第五次层析化感效果最佳的四个流分分别是RA32462,RA33444,RA98898和RA910664,其抑制率与CK对照相比分别提高了 38.74%、38.91%、44.31%、38.17%;第六次层析化感效果最佳的四个流分分别是RA324625,RA334448,RA988981和RA9106645,其抑制率与CK对照相比分别提高了 43.06%、38.56%、45.24%、46.60%。2.西芹根际区物第五次层析化感效果最佳的四个流分分别是RSA14734,RSA16248,RSA71298和RSA75356,其抑制率与CK对照相比分别提高了 36.35%、30.31%,28.35%、36.12%;第六次层析化感效果最佳的四个流分分别是RSA147341,RSA162489,RSA712982和RSA75356,其抑制率与CK对照相比分别提高了41.00%、38.41%、42.02%、36.38%。3.西芹种子第五次层析化感效果最佳的四个流分分别是SA54838、SA55884、SA714105、SA72263,其抑制率与 CK 对照相比分别提高了 35.18%、48.85%、36.00%、24.96%;第六次层析化感效果最佳的四个流分分别是SA548388、SA558845、SA71410510、SA722635,其抑制率与CK对照相比分别提高了 44.48%、46.58%、36.53%、36.46%。4.第六次层析得到的十二个最佳流分均能诱导黄瓜幼苗对黄瓜枯萎病产生抗性,其中西芹鲜根浸提液四个最佳流分的诱导抗性效果分别达到82.92%、82.26%、83.53%、80.24%;西芹根际区物浸提液四个最佳流分的诱导抗性效果分别达到81.92%、84.61%、84.24%、79.32%;西芹种子浸提液四个最佳流分的诱导抗性效果分别达到77.58%、80.21%、77.55%、78.29%。诱导试验表明:西芹鲜根与根际区物浸提液对黄瓜枯萎病的诱导抗性效果优于西芹种子浸提液对黄瓜枯萎病的诱导抗性效果。5.试验共鉴定出四类十九种具有化感作用的物质。其中,烷烃类七种:二十四烷、二十七烷、十八烷、二十八烷、二十一烷、二十三烷、四十四烷;酸类三种:棕榈酸、十八烷酸、(Z)-9-十八(碳)烯酸;酚类四种:对叔戊基苯酚、对特辛基苯酚、3,5-二叔丁基苯酚、2,6-二叔丁基对甲酚;脂类五种:油酸十八烷醇酯、14-甲基十五烷酸甲酯,棕榈酸甲酯,邻苯二甲酸二甲酯、十七酸十六甲基甲基酯。
[Abstract]:In this study, the allelopathic effects of six ketone layer on Fusarium wilt of cucumber were studied by column chromatography using celery root material (that is, celery fresh root, rhizosphere material) and celery seed as materials. The best allelopathy was identified by GC-MS. The results showed that:. 1. The four best allelopathic components of fresh root of celery 5th times were RA32462. Compared with CK, the inhibition rate of RA33444, RA98898 and RA910664 increased 38.74 and 38.91, respectively. The four flow fractions with the best allelopathic effect of 6th chromatography were RA324625ARA334448 and RA988981 and RA9106645, respectively. Compared with CK, the inhibition rate increased by 43.06% and 38.56%, respectively. RSA14734 RSA16248RSA71298 and RSA75356 were the four most sensitive components in the rhizosphere of celery (RSA14734) and RSA71298 (RSA71298). Compared with CK, the inhibition rate increased by 36.35 and 30.31, respectively. The four flow fractions with the best allelopathic effect of 6th chromatography were RSA147341RSA162489, RSA712982 and RSA75356, respectively. Compared with CK, the inhibition rate increased by 41.00% and 38.41%, respectively. The four best allelopathic components of celery seed 5th times were SA54838SSA55884ASA714105S72263. Compared with CK, the inhibition rate increased 35.18% and 48.85%, respectively, and the inhibition rate was 36.00 and 24.96, respectively. The four flow fractions with the best allelopathic effect were SA548388ASA558845, SA71410101010 and SA722635, respectively. Compared with CK, the inhibition rate increased by 44.48% and 46.58%, respectively. The 12 best flow fractions obtained by 36.46. 4.#number0# chromatography could induce cucumber seedlings to develop resistance to Fusarium wilt. The induced resistance of the four best flow fractions of fresh root extract of celery was 82.92 and 82.26, 83.53 and 80.24, respectively. The induced resistance of the four best water fractions in the rhizosphere of celery reached 81.92 and 84.61and 84.2479.32, respectively. The induced resistance of the four best fractions of celery seeds was 77.58% and 80.21%, respectively. 78.29. Induction tests show that:. The induced resistance of fresh root and rhizosphere extract of celery to cucumber wilt was better than that of seed extract of celery to cucumber wilt. 5. Four kinds of 19 allelopathic substances were identified. Among them. Seven alkanes: 24 alkanes, 27 alkanes, 18 alkanes, 28 alkanes, 21 alkanes, 23 alkanes, 44 alkanes; Three kinds of acids: palmitic acid, 18 alkanoic acid, ZC9- 18 (carboxyenoic acid); Four kinds of phenols: p-tert-amylphenol, p-octyl phenol, 3-di-tert-#china_person0# phenol, 2-di-tert-#china_person1# p-cresol; Five kinds of lipids: 18 alkanolyl oleic acid methyl ester 14-methyl 15 alkanoate methyl palmitate dimethyl phthalate 17 acid 16 methyl ester.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S436.421.13

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