甲烷生理盐水对雄性SD大鼠一次性力竭运动后氧化应激水平的影响

发布时间：2018-02-10 02:14

本文关键词： 甲烷生理盐水氧化应激一次性力竭运动　出处：《上海体育学院》2017年硕士论文　论文类型：学位论文

【摘要】：研究目的:探究运动前后腹腔注射甲烷生理盐水对雄性SD大鼠一次性力竭运动后氧化应激损伤的影响,为甲烷生理盐水在运动科学领域的应用提供实验依据。研究方法:21只清洁级健康雄性SD大鼠,2月龄,体重200~250g,适应性喂养1周。按体重配对随机分为运动安慰剂组(TP)、甲烷水恢复组(TM)、甲烷水预防恢复组(PTM),每组7只。三组大鼠进行10min速度为15m/min的适应性跑台训练,休息两天后,PTM组大鼠于运动前5min按10ml/kg体重的剂量腹腔注射甲烷生理盐水,TP、TM组大鼠以相同的剂量和方法给予生理盐水干预。三组同时进行跑台坡度为0°,速度为35m/min的一次性力竭跑台运动。判定力竭后记录其运动时间,并即刻给予TM、PTM组按10ml/kg体重的剂量腹腔注射甲烷生理盐水,TP组大鼠以相同的剂量和方法给予生理盐水干预。力竭运动结束后10min和3h分别于眼内眦和腹主静脉处取血,检测血常规、血糖及乳酸浓度,其余全血于肝素钠抗凝管中混匀,分离血浆,检测血中尿素氮、总抗氧化物浓度和肌酸激酶、谷丙转氨酶、谷草转氨酶、超氧化物歧化酶、髓过氧化物酶活性。所有实验数据均采用SPSS17.0软件进行处理和分析。实验结果以p0.05表示差异具有统计学意义。研究结果:(1)与TP组、TM组比较,PTM组大鼠的运动时间显著延长(*p0.05,#p0.05)；(2)与TP组相比,TM组在运动结束后10min,BPC、PCT升高(**p0.01)；在运动结束后3h,RBC升高(*p0.05)。与TM组相比,PTM组在运动结束后10min,WBC、Hb降低(#p0.05),在运动结束后3h,WBC、RBC、HCT、Hb均降低；(3)运动结束后10min和3h时,三组大鼠的血糖、血乳酸、尿素氮均无统计学差异。TM组大鼠血肌酸激酶与TP组、PTM组相比,在运动结束后10min时无差异,运动结束后3h显著升高(*p0.05,##p0.01)；(4)三组大鼠的血谷丙转氨酶在运动结束后10min和3h时均无统计学差异,TM组大鼠血谷草转氨酶与TP组、PTM组相比,在运动结束后10min时无差异,运动结束后3h显著升高(*p0.05,#p0.05)；(5)TM组大鼠血髓过氧化物酶在运动结束后10min时显著高于TP组(**p0.01),在运动结束后3h时均显著高于TP组、PTM组(**p0.01,##p0.01)；(6)在运动结束后10min时,PTM组大鼠丙二醛、超氧化物歧化酶和总抗氧化物均显著低于TP组、TM组(*p0.05,#p0.05)；在运动结束后3h时,TM组大鼠与TP组相比超氧化物歧化酶显著升高(**p0.01)、总抗氧化物有升高的趋势,PTM组大鼠超氧化物歧化酶显著高于TP组(**p0.01),总抗氧化物显著高于TP组和TM组(**p0.01,#p0.05)。研究结论:(1)运动前腹腔注射甲烷生理盐水可以显著延长雄性SD大鼠运动至力竭的运动时间。(2)运动后腹腔注射甲烷生理盐水可能影响了细胞膜功能的恢复,在一定程度上降低了大鼠的抗氧化能力。(3)运动前后腹腔注射甲烷生理盐水在一定程度上也降低了机体的总抗氧化能力。
[Abstract]:Objective: to investigate the effects of intraperitoneal injection of methane-saline on oxidative stress injury in male SD rats after exhaustive exercise before and after exercise. To provide experimental basis for the application of methane-saline in the field of sports science. Methods: twenty one healthy male SD rats of clean grade were 2 months old. The rats were randomly divided into the exercise placebo group, the methane water recovery group and the methane water recovery group, with 7 rats in each group. The rats in the three groups were trained on an adaptive platform with a speed of 15 m / min for 10 minutes. After two days of rest, rats in PTM group were injected intraperitoneally with 10 ml / kg body weight of 10 ml / kg body weight at the dose of 10 ml / kg body weight and treated with the same dose and method of normal saline at 5 min before exercise. The three groups were treated with the same dose and method of normal saline, and the three groups were treated with a running platform slope of 0 掳and a speed of 35 m / min. One time exhaustive treadmill movement. After exhaustion, record the time of movement, The rats in the TMN PTM group were given 10 ml / kg body weight intraperitoneal injection of methane-saline and TP group with the same dose and method. Blood samples were taken from the medial canthus and the abdominal main vein at 10 min and 3 h after exhaustive exercise, and the blood routine was detected. Blood glucose and lactate concentration were mixed in heparin sodium anticoagulant tube, plasma was separated, blood urea nitrogen, total antioxidant concentration and creatine kinase, alanine aminotransferase, glutamic oxalacetic transaminase, superoxide dismutase were detected. Myeloperoxidase activity. All experimental data were processed and analyzed by SPSS17.0 software. The results showed significant difference in p0.05. The results of the study showed that the exercise time of rats in TP group was significantly higher than that in TP group (P < 0. 05). Compared with TP group, compared with TP group, compared with TP group, the increase of BPC-PCT in TM group was significantly higher than that in TP group 10 min after exercise, and the RBC increased at 3 h after exercise. Compared with TM group, 10 mins after exercise, 10 mins after exercise and 3 h after exercise, the levels of BBCCHb in PTM group were lower than those in TP group, and the levels of HCTHb were decreased at 10min and 3h after exercise. There was no significant difference in blood glucose, blood lactic acid and urea nitrogen among the three groups. There was no difference in creatine kinase between TM group and TP group at 10 min after exercise. There was no significant difference in serum alanine aminotransferase between TM group and TP group at 10 min and 3 h after exercise. There was no significant difference in serum alanine aminotransferase between TM group and TP group at 10 min after exercise. 3 h after exercise, there was a significant increase in serum myeloperoxidase (myeloperoxidase) in the TM group than that in the TP group at 10 min after exercise, and was significantly higher than that in the TP group at 3 h after the end of exercise. At 3 h after exercise, it was significantly higher than that in the TP PTM group and ##p0.01D group) at 10 min after the end of exercise, malondialdehyde (MDA) in PTM group was significantly higher than that in the PTM group (P < 0.05), and the level of malondialdehyde in the PTM group was significantly higher than that in the PTM group at 3 h after the end of exercise. Superoxide dismutase (SOD) and total antioxidants were significantly lower than those in TP group (TM group) and #p0.05G group (P < 0.05), and at 3 h after exercise in TM group compared with TP group, superoxide dismutase (SOD) in TM group was significantly higher than that in TP group (P 0.01), and total antioxidants in PTM group had a tendency to increase superoxidation. The activity of dismutase was significantly higher than that of TP group, and the total antioxidant content was significantly higher than that of TP group and TM group. Conclusion the intraperitoneal injection of normal saline can significantly prolong the exercise time from exercise to exhaustion in male Sprague-Dawley rats before exercise. Injection of normal saline into the cavity may affect the recovery of cell membrane function. To a certain extent, the anti-oxidation ability of rats was decreased by intraperitoneal injection of methane-saline before and after exercise, and the total antioxidant capacity was also decreased to a certain extent.
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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