微压氧对大强度训练大鼠氧化应激和毛细血管再生相关因子的影响

发布时间：2018-03-04 12:35

本文选题：微压氧　切入点：大强度训练　出处：《上海体育学院》2017年硕士论文　论文类型：学位论文

【摘要】：研究目的:本研究的目的是围绕氧化应激和毛细血管再生,从自由基的产生和清除以及毛细血管再生相关因子的基因和蛋白表达调控探讨微压氧对于大强度训练后的恢复机制,为微压氧在运动训练后恢复中的应用提供理论依据和实验支持。研究方法:本研究选用SPF级8周龄雄性SD大鼠40只,经一周的适应性训练后筛选出30只,随机分为对照组(C组)、大强度训练后安静恢复组(HQ组,26.8m/min,10°)和微压氧恢复组(HM组),每组10只。本实验采用的微压氧仓氧浓度为26%左右,压强为3.6PSI。训练周期为8周,每周训练5天,每天1小时。HM组每次训练结束后即刻放入微压氧仓恢复1小时。在第8周最后一次训练结束24小时后进行麻醉,切取左侧腓肠肌用于氧化应激指标的检测和观察腓肠肌组织形态;采用实时荧光定量PCR、Western blot技术检测大鼠腓肠肌VEGF、VEGFR2、b FGF基因和蛋白水平的变化。数据应用SPSS19.0统计软件进行处理,以P0.05认为有显著性差异。研究结果:1.大鼠力竭时间的变化:HM组大鼠跑至力竭时间显著长于HQ组(P0.05)。2.氧化应激指标的变化:HQ组肌肉MDA含量高于C组和HM组,具有显著性差异(P0.05);HM组肌肉SOD活性显著性高于C组(P0.05);HM组肌肉GSH-PX的活性高于C组和HQ组,并具有显著性差异(P0.05)。3.骨骼肌组织形态学改变:与C组相比,HQ组大鼠和HM组大鼠骨骼肌损伤,但是HM组轻于HQ组。4.微压氧对毛细血管密度及毛细血管再生相关因子表达的影响:(1)毛细血管再生相关因子表达:HQ组与C组和HM组相比VEGF蛋白相对表达升高,具有显著性差异(P0.05);HQ组VEGFR2m RNA表达显著性高于HM组(P0.05);VEGFR2蛋白的相对表达HQ组显著性高于C组和HM组(P0.05);b FGFm RNA HM组低于HQ组,具有显著性差异(P0.05);HQ组与C组和HM组相比,b FGF蛋白相对表达量增加,且差异具有统计学意义(P0.05)。(2)毛细血管密度:HM组与C组相比毛细血管密度增加,具有极显著性差异(P0.01);HM组与HQ组相比,毛细血管密度增加,具有显著性差异(P0.05);HQ组毛细血管密度显著高于C组(P0.05)。研究结论:1.大强度训练可造成大鼠骨骼肌氧化应激损伤,使其运动能力下降;而微压氧可提高骨骼肌抗氧化酶活性,减少骨骼肌脂质过氧化反应,提高运动能力;2.大强度训练可以引起骨骼肌毛细血管密度增加,而微压氧可加速新生毛细血管的生成,从而可能促进氧、营养物质以及代谢产物的运输,加速运动后的恢复。
[Abstract]:Objective: the aim of this study was to investigate the recovery mechanism of microbaric oxygen after intensive training by focusing on oxidative stress and capillary regeneration, from the production and clearance of free radicals and the regulation of gene and protein expression of factors related to capillary regeneration. To provide theoretical basis and experimental support for the application of microbaric oxygen in the recovery of exercise training. Methods: 40 male SD rats of SPF grade 8 weeks of age were selected and 30 rats were selected after one week of adaptive training. The rats were randomly divided into control group (C group), quiet recovery group (HQ group) (26.8m / min 10 掳) and micro-pressure oxygen recovery group (HM group) (10 rats in each group). The oxygen concentration and pressure of micro-pressure oxygen chamber were about 26% and 3.6 PSI.The training period was 8 weeks and the training period was 5 days per week. One hour per day. The HM group was put into the oxygen chamber for 1 hour immediately after each training, and anesthetized 24 hours after the last training in the 8th week. The left gastrocnemius muscle was removed to detect the index of oxidative stress and the tissue morphology of gastrocnemius muscle was observed. The changes of VEGFN VEGFR2b FGF gene and protein in gastrocnemius muscle of rats were detected by real-time fluorescence quantitative PCR blot. The data were processed by SPSS19.0 software. The results showed that there was significant difference between the two groups. The results showed that the time of exhaustion was significantly longer in the group of rats than in the group HQ. The changes of oxidative stress index were higher in the group of oxidative stress than in the group C and HM, the content of MDA in the muscle of the group was higher than that in the group C and HM, and the change of the index of oxidative stress was higher than that of the group of C and HM. There was significant difference in muscle SOD activity in P0.05HM group than that in C group P0.05HM group, and there was significant difference in muscle GSH-PX activity between group C and HQ group. The histomorphologic changes of skeletal muscle: compared with group C and HM group, skeletal muscle injury in HM group and HM group was significant higher than that in P0.05 HM group, and there was a significant difference in muscle GSH-PX activity between HM group and HM group, compared with group C, the muscle GSH-PX activity in HM group was significantly higher than that in group C (P 0.05). However, HM group was lighter than HQ group .4.The effect of microbaric oxygen on the expression of capillary density and capillary regeneration related factors: 1) the expression of capillary regeneration related factor in group 1: HQ was higher than that in group C and HM, and the expression of VEGF protein in group 1 was higher than that in group C and HM. The expression of VEGFR2m RNA in HM group was significantly higher than that in HM group. The expression of VEGFR2 protein in HM group was significantly higher than that in C group and HM group. The expression of VEGFR2 protein in HM group was significantly higher than that in HM group, and the expression of VEGFR2 protein in HM group was significantly higher than that in HM group (P 0.05 FGFm RNA HM group). There was significant difference in the relative expression of FGF protein between P0.05HM group and C group and HM group, and the difference was statistically significant (P 0.05. 05) the capillary density of HM group was higher than that of C group, and the capillary density of HM group was higher than that of C group. The capillary density of HM group was significantly higher than that of HQ group, and the capillary density of HM group was significantly higher than that of C group. Micropressure oxygen can increase the activity of antioxidant enzymes, reduce lipid peroxidation, and improve the exercise ability of skeletal muscle. High intensity training can increase the density of capillaries in skeletal muscle. Microbaric oxygen can accelerate the formation of new capillaries, which may promote the transport of oxygen, nutrients and metabolites, and accelerate the recovery after exercise.
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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