IRE1-JNK通路在运动导致骨骼肌细胞凋亡中的作用

发布时间：2018-03-11 01:22

本文选题：骨骼肌　切入点：细胞凋亡　出处：《北京体育大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察一次大负荷运动对大鼠骨骼肌细胞凋亡和内质网应激的影响,以及一次大负荷运动后骨骼肌细胞中JNK,IRE1,TRAF2,ASK1蛋白表达的变化,探索IRE1-JNK通道在内质网应激引起的骨骼肌细胞凋亡中的作用。方法:将雄性健康8周龄大鼠,随机分为安静对照组、运动组。运动组的运动方案为持续性下坡跑,跑台坡度为-16°,速度为16m/min,时间为90min。根据取材时间点不同分为运动后0h组,12h组,24h组,48h组,72h组。通过检测Caspase3的活性来观察细胞凋亡情况;运用western blot检测GRP78、JNK、IRE1、TRAF2、ASK1的表达,使用Gel-pro软件来进行相应的条带的灰度值分析,使用单因素方差分析法处理数据。结果:1.大负荷运动后比目鱼肌中的Caspase3的活性在12h达到峰值;与安静对照组相比,除了72h组与安静组无显著性差异之外(P0.05),其余时相的大鼠比目鱼肌Caspase3的活性都显著比安静组高(P0.05)。2.大负荷运动后比目鱼肌的GRP78的蛋白表达在12h达到峰值;与安静对照组相比,大负荷运动后比目鱼肌中的GRP78蛋白表达所有时相的浓度值都显著比安静组高(P0.05)。3.大负荷运动后比目鱼肌中的IRE1、TRAF2、ASK1、JNK蛋白表达在12h达到峰值;与安静对照组相比,大负荷运动后比目鱼肌中的IRE1、TRAF2、ASK1、JNK蛋白表达所有时相的浓度值都显著比安静组高(P0.05)。结论:一次大负荷运动可导致骨骼肌细胞凋亡,可能是由于运动引起内质网应激,激活IRE1-JNK通路所致。
[Abstract]:Aim: to observe the effects of a heavy load exercise on apoptosis and endoplasmic reticulum stress in rat skeletal muscle cells and the expression of JNKN IRE1TRAF2 + ASK1 protein in skeletal muscle cells after a heavy exercise. To explore the role of IRE1-JNK channel in apoptosis of skeletal muscle cells induced by endoplasmic reticulum stress. Methods: male healthy rats were randomly divided into quiet control group and exercise group. The slope of treadmill was -16 掳, the speed was 16m / min, and the time was 90 min.According to the different time points after exercise, they were divided into two groups: 0 h group, 12 h group, 24 h group, 48 h group, 72 h group. Apoptosis was observed by detecting the activity of Caspase3, and western blot was used to detect the expression of GRP78, JNKIRE1TRAF2, ASK1. The grayscale value of the band was analyzed by Gel-pro software, and the data were processed by single factor variance analysis. The results showed that the activity of Caspase3 in soleus muscle reached its peak at 12 h after heavy exercise, and compared with that in the quiet control group. Except that there was no significant difference between the 72 h group and the quiet group, the activity of Caspase3 in the soleus muscle of the rest rats was significantly higher than that of the rest group. The protein expression of GRP78 in the soleus muscle reached its peak at 12 h after heavy exercise, and was higher than that in the control group. The expression of GRP78 protein in soleus muscle after heavy exercise was significantly higher than that in control group (P 0.05 路3.The expression of IRE1TRAF2TRAF2ASK1 / JNK protein in soleus muscle reached its peak at 12h, compared with that in quiet control group), and the expression of IRE1TRAF2ASK1 / JNK protein in soleus muscle was significantly higher than that in control group (P < 0.05). The concentration of IRE1TRAF2ASK1 / JNK protein in soleus muscle after heavy exercise was significantly higher than that in control group (P 0.05). Conclusion: one exercise at high load can induce apoptosis of skeletal muscle cells, which may be due to endoplasmic reticulum stress induced by exercise. Activation of IRE1-JNK pathway.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

【相似文献】