罗汉果甜苷对训练大鼠骨骼肌PGC-lαmRNA及蛋白表达的影响

发布时间：2018-04-03 06:47

本文选题：罗汉果甜苷　切入点：耐力训练　出处：《现代预防医学》2017年01期

【摘要】：目的观察运动前补充罗汉果甜苷对训练大鼠骨骼肌过氧化物酶体增殖物受体共激活因子-lα(PGC-lα)mRNA及蛋白表达并分析其影响机制。方法 SD雄性大鼠30只,随机分为安静对照组(NC),运动对照组(NE),运动+罗汉果甜苷组(MOGE)。MOGE组按10.0m L/(kg·d)进行灌服给药,对照组给等量生理盐水。并且进行为期6周的训练,速度为19.3m/min,跑台坡度为5°。采用RT-PCR法和Western Blot法测定大鼠骨骼肌PGC-lαmRNA及蛋白表达。结果腓肠肌MOGE组PGC-lαmRNA表达高于NC与NE组(T=13.238,T=15.831,P0.001)。比目鱼肌NE与MOGE组PGC-lαmRNA及蛋白表达均高于NC组(T=9.818,P=0.043;T=10.387,P=0.027);。腓肠肌和比目鱼肌同组不同组织比较时,比目鱼肌NE组PGC-lαmRNA高于腓肠肌NE组(t=9.611,P=0.041);比目鱼肌NE与MOGE组PGC-lα蛋白表达均高于腓肠肌NE与MOGE组(t=6.332,P0.001)。结论 PGC-lα信号传导调节在腓肠肌和比目鱼肌中存在差异性,补充罗汉果甜苷有助于腓肠肌和比目鱼肌能量代谢的动员和提升运动耐力。
[Abstract]:Objective to observe the expression of peroxisome proliferator receptor co-activator (PGF 伪) -l 伪 -PGC-l 伪 mRNA and protein in skeletal muscle before exercise and to analyze its mechanism.Methods Thirty Sprague-Dawley male rats were randomly divided into control group (n = 30), control group (n = 30), exercise control group (n = 10), moguloside group (n = 30) and control group (n = 10 m L/(kg / d).It was trained for 6 weeks at a speed of 19.3 m / min and a slope of 5 掳.The expression of PGC-l 伪 mRNA and protein in rat skeletal muscle was determined by RT-PCR and Western Blot.Results the expression of PGC-l 伪 mRNA in gastrocnemius MOGE group was higher than that in NC and NE groups.The expression of PGC-l 伪 mRNA and protein in NE and MOGE groups were higher than that in NC group.PGC-l 伪 mRNA in NE group was higher than that in NE group, and PGC-l 伪 protein expression in NE group and MOGE group was higher than that in NE group and MOGE group.Conclusion the regulation of PGC-l 伪 signal transduction in gastrocnemius muscle and soleus muscle is different, and the supplement of Grosvenin is helpful to mobilize energy metabolism and improve exercise endurance of gastrocnemius and soleus muscles.
【作者单位】：广西师范大学体育学院;省部共建药用资源化学与药物分子工程国家重点实验室;广西师范大学漓江学院;
【基金】：国家自然科学基金项目(31460232,21562006) 广西自然科学资金面上项目(2015GXNSFAA139186) “省部共建药用资源化学与药物分子工程国家重点实验室”开放课题(CMEMR2012-B04)
【分类号】：G804.7;R87

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