过氧化氢酶基因多态性在运动员选材中应用的可行性研究

发布时间：2018-04-03 18:50

本文选题：CAT基因多态性　切入点：体成分　出处：《江西师范大学》2015年硕士论文

【摘要】：研究目的:通过分析过氧化氢基因(CAT)多态性在中国汉族运动员和对照人群中的分布特征及该位点多态性与运动员选材用生理生化指标的关联,以期从遗传学角度探寻与运动能力相关的基因标记。研究对象与方法:本研究选取了CAT基因SNP/A-89T位点。采用酶切和测序的方法分析CAT基因SNP/A-89T多态性在92名中国汉族运动员和110名汉族普通对照组中的分布特征。同时测试运动员的体成分最大摄氧量、血尿素、肌酸激酶及血象指标,试图探寻CAT基因SNP/A-89T作为运动员选材用基因指标的可行性。研究结果:(1)CAT基因SNP/A-89T在汉族普通人群中基因型分布为TT型最少,AT型次之,AA型最多。这与国内其它地区汉族人群间分布频率未见显著性差异,但与日本人群以及南美人群的基因型分布频率存在显著性差异。提示CAT基因SNP/A-89T位点在国内各地域间分布特征不存在差异,但可作为种族差异的基因标记。(2)CAT基因SNP/A-89T位点在汉族运动员和对照组中基因型频率和等位基因频率均无性别地域差异。将运动员按照级别分组后发现,女子一级运动员携A等位基因的分布频率明显高于携T等位基因的频率。按照供能系统分组,发现有氧项目女子运动员(AT+TT)型的分布频率显著高于AA型,携T等位基因的频率显著高于A等位基因。(3)CAT基因SNP/A-89T与选材用部分生理生化指标有关联。在有氧项目中,发现男子运动员AA型去脂体重显著高于(AT+TT)型运动员;男女运动员携(AT+TT)型者的血红蛋白均显著高于AA型;男子运动员中携(AT+TT)型者在VO2max(ml/min/Kg)指标显著高于AA型;女子运动员携(AT+TT)型在血尿素指标中显著低于AA型。研究结论:CAT基因SNP/A-89T携(AT+TT)型可作为有氧运动员选材用基因指标,但还需要加大样本量进一步研究。
[Abstract]:Objective: to analyze the distribution of hydrogen peroxide gene CAT polymorphism in Chinese Han athletes and the association between the polymorphism and physiological and biochemical indexes.The aim is to explore the genetic markers related to motor ability from the perspective of genetics.Methods: the SNP/A-89T locus of CAT gene was selected in this study.The distribution of CAT gene SNP/A-89T polymorphism in 92 Chinese Han athletes and 110 Han normal controls was analyzed by enzyme digestion and sequencing.At the same time, the maximal oxygen uptake, blood urea, creatine kinase and blood image index of athletes were tested to explore the feasibility of using CAT gene SNP/A-89T as a genetic index for selecting athletes.The results showed that the genotype distribution of cat gene SNP/A-89T was the lowest in TT type and second in AT type in Han population.There was no significant difference in distribution frequency between Han population in other regions of China, but there was significant difference in genotype distribution with Japanese population and South American population.It was suggested that there was no difference in the distribution of SNP/A-89T loci of CAT gene among different regions in China, but there was no gender difference in genotype frequency and allele frequency of SNP/A-89T locus of cat gene in Han athletes and controls.The distribution frequency of A allele was obviously higher than that of T allele.According to the energy supply system, it was found that the distribution frequency of AT TTT type in aerobic women athletes was significantly higher than that of AA type, and the frequency of carrying T allele was significantly higher than that of A allele. The SNP/A-89T of catalase gene was correlated with some physiological and biochemical indexes of material selection.In aerobic events, it was found that the body weight of male athletes with AA type fat removal was significantly higher than that of AT type athletes, that of male and female athletes with AT TTT type was significantly higher than that of AA type, that of male athletes with AT TTT type was significantly higher than that of AA type, that of male athletes with AT TTT type was significantly higher than that of AA type.The blood urea index of female athletes was significantly lower than that of AA type.Conclusion the SNP/A-89T / AT TTT type of the 1: cat gene can be used as a genetic index for the selection of aerobic athletes, but there is still a need for further research on the sample size.
【学位授予单位】：江西师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：G808.18

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