力竭运动恢复期大鼠纹状体DA含量及其受体相对表达量的变化

发布时间：2018-04-24 16:42

本文选题：力竭运动 + 运动性中枢疲劳　；参考：《南京体育学院》2015年硕士论文

【摘要】：目的:本实验以大鼠一次性力竭运动为动物模型。了解力竭运动前后及恢复期不同时段大鼠纹状体中DA含量及D1DR和D2DR相对表达量的变化情况,观察力竭运动前后及恢复期纹状体中DA含量及其受体相对表达量的变化规律,分析DA与D1DR、DA和D2DR以及D1DR和D2DR之间可能存在的相互关系,探讨运动性疲劳发生及其24小时恢复期内纹状体DA及D1DR和D2DR的调控作用,为更深入研究运动性中枢疲劳产生及其恢复机制提供实验依据。方法:将36只SD大鼠适应性喂养一周后随机分成安静组、力竭运动即刻组、力竭运动后3小时组、力竭运动后8小时组、力竭运动后15小时组和力竭运动后24小时组,每组6只,共6组。为了消除生物节律的影响,所有力竭运动组大鼠均在上午进行一次性力竭游泳运动,运动结束后于相应时间点断头取脑,冰面上剥离纹状体。安静组与力竭运动组大鼠同一时间采样。样品采集完成后用ELISA测试盒测试大鼠纹状体DA含量,用Western Blot检测大鼠纹状体D1DR和D2DR的相对表达量。结果:1、力竭运动恢复期大鼠纹状体DA含量的变化:与安静组相比,力竭运动即刻组大鼠纹状体DA含量水平显著性下降(p0.001),力竭运动后恢复8小时、15小时、24小时大鼠纹状体DA含量显著增加(p0.001);且力竭运动恢复15小时达到峰值;与力竭运动即刻组相比,恢复3小时、8小时、15小时、24小时大鼠纹状体DA含量水平均显著性升高且p0.0001。2、力竭运动恢复期大鼠纹状体D1DR相对表达量变化:与安静组相比,力竭运动即刻组大鼠纹状体D1DR相对含量显著性降低(p0.001),力竭运动3小时组、8小时组、15小时组、24小时组均高于与安静组水平,且差异具有显著性(P0.001);与力竭运动即刻组相比,力竭运动后恢复3小时组、8小时组、15小时组和24小时组大鼠纹状体D1DR相对表达量均有所增加且P0.001。3、力竭运动恢复期大鼠纹状体D2DR相对表达量的变化:与安静组相比,力竭运动即刻组大鼠纹状体D2DR相对表达量明显下降(P0.001),恢复8小时达到24小时恢复期的最高值(p0.05),随后15小时开始下降接近于安静组水平;与力竭运动后即刻组相比,力竭运动恢复3小时组、8小时组、15小时组和24小时组大鼠纹状体D2DR的相对表达量均出现增加且p0.001。4、力竭运动恢复期大鼠纹状体DA含量与D1DR相对表达量存在高度相关关系(r=0.71)且具有统计学意义(p0.001);DA含量与D2DR表达量之间的相关性较低弱(r=0.32),无统计学意义(p0.05);D1DR和D2DR之间的存在高度正相关关系(r=0.83,p0.001)。结论:1、纹状体中DA具有双向调节作用。当运动至力竭后纹状体DA含量降低可通过间接通路抑制机体的运动能力,参与运动性中枢疲劳的发生;在24小时恢复期内DA含量达到峰值后缓慢下降但仍显著高于安静水平,可激活直接通路活性,使其恢复到正常水平,从而使中枢兴奋性逐渐加强,机体的运动能力得以保持甚至提高。2、运动至力竭时,纹状体D1DR和D2DR相对表达量均显著低于安静时水平,有利于保护神经元免受兴奋毒性的损害,从而起到机体保护性抑制的作用。在恢复期纹状体D1DR和D2DR相对表达量上调,可激活间接通路影响锥体外系实现对机体运动功能的调控,使机体的疲劳程度减轻。3、在运动疲劳产生及其恢复过程中,大鼠纹状体DA含量的增加可刺激D1DR的表达,而D2DR表达量主要受D1DR表达量介导。
[Abstract]:Objective: To investigate the changes of the content of DA and the relative expression of D1DR and D2DR in the striatum of rats before and after the exhaustion exercise, and to observe the changes of the content of DA and the relative expression of the receptor in the striatum before and after the exhaustion exercise, and to analyze the DA and D1DR, DA, DA. The possible interaction between D2DR and D1DR and D2DR and the regulation of the striatal DA and D1DR and D2DR in the 24 hour recovery period are discussed in order to provide the experimental basis for the further study of the mechanism of sports central fatigue and its recovery mechanism. Methods: 36 SD rats were fed for one week and randomly divided into silence. Group, exhausted exercise immediate group, 3 hours after exhaustive exercise group, 8 hours after exhaustive exercise, 15 hours after exhaustion exercise and 24 hours after exhaustive exercise, 6 in each group, 6 groups. In order to eliminate the influence of biological rhythm, all the rats in the exhausted exercise group were all exhausted swimming in the morning, after the exercise ended at the corresponding time point. Take the brain and peel the striatum on the ice surface. The rats in the quiet group and the exhaustive exercise group were sampled at the same time. After the sample collection was completed, the DA content of the striatum was measured by ELISA test box, and the relative expression of D1DR and D2DR in the striatum of the rats was detected by Western Blot. Results: 1, the changes in the DA content in the striatum of the exhaustive exercise rats were compared with those in the quiet group. The level of DA in the striatum of rats was significantly decreased (p0.001), after exhaustive exercise, the content of DA in the striatum was significantly increased (p0.001) in 24 hours after exhaustive exercise (p0.001), and the exhaustion movement recovered for 15 hours and reached the peak value, compared with the immediate group of exhaustive exercise, 3 hours, 8 hours, 15 hours, and 24 hours of DA in the striatum of rats. The mean significant increase of water volume and p0.0001.2, the relative expression of D1DR in the striatum of the rats in the recovery period of exhaustion: compared with the quiet group, the relative content of D1DR in the striatum was significantly lower (p0.001), the 3 hour group of exhaustive exercise, the 8 hour group, the 15 hour group, and the 24 hour group were all higher than those in the quiet group, and the difference had the difference. Significance (P0.001); compared with the immediate group of exhaustive exercise, 3 hours after exhaustive exercise, 8 hour group, 15 hour group and 24 hour group, the relative expression of D1DR in the striatum of rats increased and P0.001.3, the relative expression of D2DR in the striatum of the exhausted exercise rats was changed: compared with the quiet group, the striatum was D in the immediate group of exhaustive exercise group. The relative expression of 2DR decreased significantly (P0.001) and reached the highest value of the 24 hour recovery period of 8 hours (P0.05), and then decreased close to the level of the quiet group after 15 hours. The relative expression of D2DR in the striatum of the 15 hour group and the 24 hour group increased in the 8 hour group compared with the immediate group after the exhaustion movement. And p0.001.4, there was a high correlation between the DA content in the striatum and the relative expression of D1DR (r=0.71) in the recovery period of exhaustive exercise (r=0.71) and had statistical significance (p0.001), and the correlation between the content of DA and the expression of D2DR was low (r=0.32), and there was no statistical significance (P0.05), and there was a highly positive correlation between D1DR and D2DR (r=0.83,). 1, DA in striatum has a bidirectional regulation effect. The decrease of DA content in striatum after exercise to exhaustion can inhibit the exercise ability of the body through indirect pathway and participate in the occurrence of motor central fatigue. In the 24 hour recovery period, the content of DA decreases slowly but is still significantly higher than the quiet level, which can activate the direct pathway activity and make it restorable. At the normal level, the central excitability is gradually strengthened and the motor ability of the body can be maintained or even increased by.2. When the movement to exhaustion, the relative expression of D1DR and D2DR in the striatum is significantly lower than that in the quiet time. It is beneficial to protect the neurons from the damage of the excitotoxicity and thus play the role of protective inhibition in the body. The relative expression of D1DR and D2DR is up-regulated, which can activate the indirect pathway to regulate the exercise function of the body by the extrapyramidal system and reduce the fatigue degree of the body by.3. The increase of the DA content in the striatum of the rat can stimulate the expression of D1DR, while the expression of D2DR is mainly mediated by the expression of D1DR in the process of movement fatigue and recovery.

【学位授予单位】：南京体育学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：G804.7

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