补糖对EAMD者生殖激素与相关能量代谢激素的影响研究

发布时间：2018-05-15 04:30

本文选题：运动性月经周期紊乱 + 补糖　；参考：《上海体育学院》2017年硕士论文

【摘要】：研究目的:本研究立足于可利用能量假说,采用补糖(不干预受试者运动训练与生活习惯)的方式对运动性月经周期紊乱(EAMD)进行干预,分析补糖对机体能量状况的影响以及机体能量状况的改变对生殖激素、相关应激激素和能量代谢激素的作用,并尝试探讨其相关性和影响机制,以观察补糖对EAMD的防治效果。研究方法:招募14名EAMD受试者随机分为补糖组与对照组,每组各7名,补糖组进行连续三个月经周期的补糖干预。在试验前后均对所有受试者进行身体成份与能量平衡状况的评估。在试验开始前一个月经周期和试验第三个月经周期采集所有受试者卵泡晚期和黄体中期的静脉血液,以检测促性腺激素释放激素(Gn RH)、促卵泡激素(FSH)、黄体生成素(LH)、雌二醇(E_2)、孕酮(P)、睾酮(T)、胃饥饿素(Ghrelin)、瘦素(Leptin)、皮质醇(C)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。通过比较试验前后受试者能量平衡状况和相关血液指标的变化,拟探讨补糖干预对EAMD的作用效果。研究结果:1.试验前所有受试者均为能量负平衡(补糖组:-468.9±367.1 kcal;对照组:-445.6±304.8 kcal),试验后补糖组能量平衡由负转正(132.5±107.8 kcal),组间、组内比较有统计学意义(p0.05),对照组能量状况无明显变化。2.试验后补糖组卵泡期E_2、黄体期P水平上升(E_2:52.4±6.5~88.5±41.1;P:6.7±7.2~7.7±5.1)与对照组比较有统计学意义(p0.05);除E_2、P外,Gn RH、FSH、LH、T、Ghrelin、Leptin、C、TC、TG、HDL-C、LDL-C未发现明显变化。3.相关性分析显示GnRH和TC、LDL-C具有正相关性,LH、T均与TC、HDL-C具有正相关性,FSH、P均与HDL-C具有正相关性。研究结论:1.补糖使EAMD者的能量负平衡转变为正平衡,说明补糖对机体能量缺乏有良好的干预效果,能够逆转能量负平衡情况。2.补糖对相关生殖激素E_2、P的分泌有一定的促进作用,使卵泡期E_2和黄体期P分泌上升,对EAMD者恢复正常的月经周期有一定帮助。3.本研究相关生殖激素与胆固醇之间具有一定的相关关系:其中,GnRH和TC、LDL-C具有中度正相关性,LH、T均与TC、HDL-C具有正相关性,FSH、P均与HDL-C具有正相关性,进一步证实胆固醇与HPO轴各级组织所分泌的生殖激素的关系,及其对正常月经周期的影响作用。
[Abstract]:Objective: this study was based on the hypothesis of available energy and intervened in exercise menstrual cycle disorder (EAMDD) by using sugar supplement (not interfering with exercise training and living habits of subjects). The effects of glucose supplementation on energy status and the effects of energy status on reproductive hormones, related stress hormones and energy metabolic hormones were analyzed, and the correlation and mechanism were discussed in order to observe the effect of glucose supplementation on the prevention and treatment of EAMD. Methods: fourteen subjects of EAMD were randomly divided into two groups: the glucose supplement group (n = 7) and the control group (n = 7). All subjects were evaluated for body composition and energy balance before and after the trial. Venous blood was collected from all subjects during the late follicular and middle luteal stages of the first menstrual cycle and the third menstrual cycle of the trial. To detect gonadotropin releasing hormone (Gn RHH), follicle stimulating hormone (FSH), luteinizing hormone (LHN), estradiol (E), progesterone (P), testosterone (T), ghrelin, leptin (Leptinn), cortisol (C), total cholesterol (TC), triglyceride (TGN), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (HDL-C). Degree lipoprotein cholesterol (LDL-C). The effects of glucose supplementation on EAMD were studied by comparing the changes of energy balance and related blood indexes before and after the experiment. The result of the study was: 1. Before the experiment, all the subjects were in negative energy balance (w -468.9 卤367.1 kcala in the glucose supplement group and in the control group: -445.6 卤304.8 kcal.After the experiment, the energy balance in the glucose supplement group turned from negative to positive (132.5 卤107.8 kcal-1). There was significant difference between the two groups (p 0.05), but there was no significant change in the energy status of the control group. Compared with the control group, the levels of E _ 2: 52.4 卤6.5 ~ 88.5 卤41.1% P: 6.7 卤7.2 卤7.7 卤5.1) in the glucose supplement group were significantly higher than those in the control group (P < 0.05). Correlation analysis showed that there was a positive correlation between GnRH and TCU LDL-C. Both of them were positively correlated with TCU HDL-C. Both FSHP and HDL-C were positively correlated. Conclusion: 1. The negative energy balance of EAMD patients was changed into positive equilibrium by sugar supplementation, which indicated that glucose supplementation had a good intervention effect on energy deficiency, and could reverse the negative energy balance. 2. Glucose supplementation can promote the secretion of E2P, and increase the secretion of E _ 2 and P in follicular phase, which is helpful to the recovery of normal menstrual cycle in patients with EAMD. There was a positive correlation between GnRH and TCU LDL-C in this study. There was a positive correlation between GnRH and TCU LDL-C and HDL-C. FSHP was positively correlated with HDL-C. To further confirm the relationship between cholesterol and reproductive hormones secreted by HPO axis and its effect on normal menstrual cycle.
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7

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