低氧环境下间歇耐力运动对肥胖大鼠体成分、瘦素、胰岛素抵抗的影响

发布时间：2018-06-20 11:34

本文选题：肥胖大鼠 + 低氧训练　；参考：《安徽师范大学》2017年硕士论文

【摘要】：研究目的研究低氧环境下间歇耐力运动对肥胖大鼠体成分、瘦素、胰岛素敏感性的影响,探讨低氧环境下间歇耐力运动减体重的可能原因,为后期研究低氧减体重提供理论依据和方法指导。研究方法6周龄雄性SPF级SD大鼠203只,随机挑选25只用普通饲料喂养,178只用高脂饲料喂养,18周后,按高脂饲养大鼠体重增量大于普通饲养大鼠体重增量均值加上1倍标准差作为肥胖标准筛选出肥胖大鼠。随后从普通饲料喂养大鼠中挑选8只作为对照组,即常氧安静组(NS组),从经过适应性训练的肥胖大鼠中筛选出56只平均分为7组,保证每组大鼠体重均值基本一致,随机分为肥胖低住安静组(LNS组)、肥胖高住安静组(HNS组)、肥胖低住低练持续组(LLC组)、肥胖低住低练间歇组(LLI组)、肥胖低住高练间歇组(LHI组)、肥胖高住低练间歇组(HLI组)、肥胖高住高练间歇组(HHI)。训练组大鼠中持续训练组进行4周动物跑台持续耐力训练,间歇训练组进行4周间歇耐力训练。持续组一次性完成训练,间歇组分为两次完成,两次训练间隔6小时。常氧环境下训练每周5天每天60分钟,强度为22米/分、低氧环境下训练每周5天每天60分钟,强度为20米/分。试验期间每周一上午在大鼠禁食12小时、禁水2小时称量大鼠体重,每周日晚8点收集大鼠饲料,计算大鼠的食物摄入量。运动干预4周后,大鼠休息12小时后处死大鼠,腹主动脉取血待测,并取大鼠肾周、附睾脂肪、腓肠肌、心肌在生理盐水中洗净、滤纸吸干水分电子天平称量重量。研究结果1.实验四周末HHI组、LHI组、HLI组大鼠体重均显著性低于LNS组与HNS组(P0.05),HHI组体重显著低于LLC组和LLI组(P0.05)。HHI组每一周的体重降幅显著高于HLI组和LHI组(P0.05),HHI组的总体重降幅高于LLC组和LLI组但差异不显著(P0.05)。2.四周实验后HHI组、HLI组、LHI组大鼠肾周脂肪和附睾脂肪均低于HNS组与LNS组,HHI组肾周脂肪、附睾脂肪相对值显著低于LLC组(P0.05);HHI组、HLI组、LHI组大鼠脂体比低于HNS组与LNS组但无显著差异(P0.05),所有低氧间歇运动组大鼠体脂比均低于常氧运动组。HHI组的体脂比显著低于HLI组、LHI组(P0.05)。3.四周实验后,HHI组、HLI组LHI组血清总胆固醇水平低于LNS组、各低氧运动组的总胆固醇水平亦低于常氧运动组差异不显著(P0.05);血清甘油三酯(TG)含量HHI组、HLI组、LHI组均于LNS组,HHI组、LHI组甘油三酯水平略低于LLC组、LLI组但差异不显著(P0.05);实验四周后,HHI组、LHI组、HLI组低密度脂蛋白水平低于LNS组,HHI组低密度脂蛋白水平低于LLC组LLI组;四周实验后HHI组、LHI组HLI组高密度脂蛋白水平高于LNS组,LHI组HLI组高密度脂蛋白水平高于LLC组。4.实验四周后LHI组和HHI组的瘦素水平低于LNS组和HNS组,HHI组、HLI组、LHI组的瘦素水平低于LNS组与HNS组但无显著性差异(P0.05)。低氧运动组的胰岛素水平低于常氧运动组。4周实验后HHI组HLI组血糖水平低于LNS组,HHI组、HLI组、LHI组的血糖水平低于LLC组LLI组;实验4周后,HHI组、LHI组的胰岛素指数低于LNS组HNS组,但与HNS组无显著性差异,HHI组LHI组和LHI组的胰岛素抵抗指数低于LNS组,低氧运动组胰岛素抵抗指数低于常氧运动组,但无显著性差异(P0.05)。结论从降低体重的幅度来看,HHI组HLI组LHI组LLI组;低氧环境下间歇耐力运动对肥胖机体体成分有良好影响,均能有效减少肥胖大鼠脂肪含量,其中高住高练间歇训练方式对体成分影响最佳;低氧环境下间歇耐力运动均能降低肥胖大鼠血清TG、LDL-C水平,提高HDL-C水平,对血脂产生良好影响;低氧环境下间歇耐力运动能够有效的降低肥胖机体的瘦素水平,对肥胖机体的瘦素水平有较好的影响;低氧环境下间歇耐力运动能够降低肥胖机体的胰岛素水平及胰岛素指数,影响肥胖机体的胰岛素敏感性。
[Abstract]:Objective to study the effect of intermittent endurance exercise on body composition, leptin and insulin sensitivity in hypoxic rats and to explore the possible causes of weight loss in intermittent endurance exercise under hypoxic environment, and provide theoretical basis and method guidance for the later study of hypoxic weight reduction. 203 male SPF rats of 6 weeks' age were selected to select 25 randomly. After only feeding with ordinary feed, 178 only were fed with high fat diet. After 18 weeks, the weight increment of rats fed by high fat feeding rats was larger than that of normal feeding rats plus 1 times the standard deviation of 1 times as a standard of obesity. Then 8 of the rats were selected as control group, that is, the normal oxygen quiet group (group NS). The average weight of 56 obese rats was divided into 7 groups, which ensured that the average weight of the rats in each group was basically the same. It was randomly divided into the obese and low living quiet group (group LNS), the obese and quiet group (group HNS), the fat low living and low practice group (group LLC), the fat low living and low training intermittent group (group LLI), the fat low high practice interval group (group LHI), the obesity and low practice. In the intermittent group (group HLI), the obese and high training interval group (HHI). The continuous training group was trained for 4 weeks in the training group and the intermittent training group had 4 weeks of intermittent endurance training. The continuous group was completed once in one time, the intermittent group was divided into two times and the two training interval was 6 hours. The training group was trained for 60 minutes a day for 5 days a week. The intensity was 22 m / min and 5 days a week, 60 minutes a week and 20 m / min in the low oxygen environment. The rats were fasted for 12 hours in rats every Monday morning, and the rats were weighed for 2 hours without water. The rat feed was collected at 8 o'clock every Sunday night, and the food intake of rats was calculated. After 4 weeks of exercise intervention, rats were killed and abdominal initiative after rest 12 hours after the rest of the rats. Abdominal initiative was taken. The body weight of the HHI group, LHI group and HLI group was significantly lower than that of group LNS and HNS group (P0.05), and the weight of group HHI was significantly lower than that of group LLC and LLI group (P0.05).HHI group every week in group HHI. The weight reduction of body weight was significantly higher than that of group HLI and group LHI (P0.05). The total weight loss of group HHI was higher than that of group LLC and LLI group, but the difference was not significant (P0.05) in group HHI, HLI group and LHI group was lower than that in HNS group and epididymal fat, and the relative value of epididymal fat was significantly lower than that of group of HNS and epididymis. The fat body ratio in the rats was lower than that of the HNS group and the LNS group (P0.05). The body fat ratio of all hypoxic intermittent exercise groups was lower than that of the.HHI group of the normooxy group was significantly lower than that of the HLI group. After the LHI group (P0.05).3. around the experiment, the serum total cholesterol level of the HHI group and HLI group LHI group was lower than that of the LNS group, and the total cholesterol level in the hypoxia exercise group was also low. There was no significant difference in the aerobics group (P0.05), the content of serum triglyceride (TG) in group HHI, group HLI and LHI in group LNS, HHI group and LHI group were slightly lower than that of group LLC, and LLI group was not significant (P0.05), and the level of low density lipoprotein in HHI group, group, and low density lipoprotein was lower than that in the group of LLI, and the level of low density lipoprotein was lower than that of the group. The level of HDL in group HLI and group LHI was higher than that of group LNS, and the level of high density lipoprotein in group HLI of group LHI was higher than that of group LLC and.4. experiment in group LHI, and the level of leptin in group HLI and HHI group was lower than that of LNS group and HNS group. The level of blood glucose in group HLI of group HHI was lower than that of group LNS after.4 week in group HLI. The blood glucose level of group HHI, HLI and LHI was lower than that of group LLI. After 4 weeks, the insulin index of HHI group and LHI group was lower than that of LNS group, but there was no significant difference between the group and the group, the insulin resistance index was lower than that of the group and the hypoxia group. The insulin resistance index in the exercise group was lower than that of the normoxic exercise group, but there was no significant difference (P0.05). Conclusion from the margin of weight reduction, the group LHI of group HLI was LLI in group HHI, and the intermittent endurance exercise under the low oxygen environment had a good effect on the body composition of the obese body, which could effectively reduce the fat content in the obese rats. The effect of body composition is the best. Intermittent endurance exercise in low oxygen environment can reduce the serum TG, LDL-C level and increase the level of HDL-C in obese rats. The intermittent endurance exercise can effectively reduce the level of leptin in obese body, have a good influence on the leptin level of obese body, and the interval of hypoxia is intermittent. Endurance exercise can reduce the insulin level and insulin index of obese body, and affect the insulin sensitivity of obese body.
【学位授予单位】：安徽师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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