常氧和低氧运动对Ⅱ型糖尿病大鼠糖脂代谢影响的研究
发布时间：2018-07-16 18:26
【摘要】：1.目的通过观察常氧和低氧运动对Ⅱ型糖尿病大鼠糖脂代谢相关指标的影响,探讨常氧和低氧运动对Ⅱ型糖尿病的作用及可能机制。2.方法选取60只6~8周龄雄性SD大鼠作为实验对象,随机取10只作正常对照组,其余经高糖高脂饲料喂养4周后,腹腔注射链脉佐菌素(STZ)建立Ⅱ型糖尿病大鼠模型(空腹血糖"g16.7mmol·L-1为成模标准)。将建模成功的Ⅱ型糖尿病大鼠再随机分为糖尿病组、糖尿病常氧运动组和糖尿病低氧运动组,每组各10只。后两组分别进行为期4周跑台运动训练(坡度0°,速度15m·min-1,60 min·d-1,6d·W-1),糖尿病低氧运动组在低氧帐篷(模拟海拔2500m,氧浓度15.0%的氧环境)中运动。末次运动后,所有大鼠禁食不禁水12h,经麻醉后,腹腔取血、肝脏、肾周脂肪。测定大鼠空腹血糖(FBG)、空腹胰岛素(FINS)、血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL-C)、肝脏组织中胰岛素受体蛋白(InsR)、葡萄糖激酶(GCK)、内脏脂肪组织中葡萄糖转运蛋白(GLUT-4)的含量。3.结果(1)经过4周运动干预,糖尿病常氧运动组和糖尿病低氧运动组大鼠症状较糖尿病组均有所减轻,其中,糖尿病低氧运动组效果较为明显。(2)与正常对照组相比,糖尿病组大鼠血清TC、TG和LDL-C均非常显著升高(P0.01);与糖尿病组相比,糖尿病常氧运动组大鼠血清LDL-C及TC均非常显著降低(P0.01),糖尿病低氧运动组大鼠血清LDL-C、TC和TG均非常显著降低(P0.01),HDL明显升高(P0.05);与糖尿病低氧运动组相比,糖尿病常氧运动组大鼠血清TC及LDL-C均较高(P0.05),HDL较低(P0.05),TG明显较高(P0.05)。(3)与正常对照组相比,糖尿病组FBG显著升高(P0.05);与糖尿病组相比,糖尿病常氧运动组和糖尿病低氧运动组FBG均非常显著降低(P0.01);与糖尿病低氧运动组相比,糖尿病常氧运动组FBG较高(P0.05)。(4)与正常对照组相比,糖尿病组FINS显著升高(P0.05);与糖尿病组相比,糖尿病常氧运动组和糖尿病低氧运动组FINS均非常显著降低(P0.01);与糖尿病低氧运动组相比,糖尿病常氧运动组FINS明显升高(P0.05)。(5)与正常对照组相比,糖尿病组肝脏中InsR、GCK以及内脏脂肪组织中GLUT-4含量均非常显著降低(P0.01);与糖尿病组相比,糖尿病常氧运动组和糖尿病低氧运动组肝脏组织中InsR、GCK以及GLUT-4含量均明显升高(P0.05);与糖尿病低氧运动组相比,糖尿病常氧运动组GCK含量显著降低(P0.05)。4.结论常氧和低氧运动均可降低Ⅱ型糖尿病大鼠空腹血糖及空腹胰岛素浓度,减少血清TG、TC和LDL-C含量,升高HDL含量,增加肝组织中InsR,GCK以及内脏脂肪组织中GLUT-4的含量,有利于促进Ⅱ型糖尿病大鼠糖脂代谢紊乱的恢复,且低氧运动干预效果优于常氧运动。
[Abstract]:1. Objective to observe the effects of normoxic and hypoxic exercise on glucose and lipid metabolism in type 2 diabetic rats, and to explore the effect of normoxic and hypoxic exercise on type 2 diabetes mellitus and its possible mechanism. Methods 60 male Sprague-Dawley (SD) rats aged 6 weeks and 8 weeks old were selected as experimental objects, 10 rats were randomly selected as normal control group, and the rest were fed with high sugar and high fat diet for 4 weeks. The rat model of type 2 diabetes was established by intraperitoneal injection of streptozotocin (STZ). Fasting blood glucose (g16.7mmol L-1) was used as the model standard. The successful type 2 diabetic rats were randomly divided into diabetic group, diabetic normoxic exercise group and diabetic hypoxic exercise group with 10 rats in each group. The latter two groups were trained in treadmill training for 4 weeks (slope 0 掳, speed 15m min-1 60 min d-1d W-1), and diabetic hypoxic exercise group exercised in hypoxic tents (simulated altitude 2500m, oxygen concentration 15.0%). After the last exercise, all rats fasted for 12 hours. After anaesthesia, blood, liver and perirenal fat were taken from abdominal cavity. Fasting blood glucose (FBG), fasting insulin (fins), serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL-C), insulin receptor protein (InsR), glucose kinase (GCK) and glucose in visceral adipose tissue were measured in rats. The content of transporter protein (GLUT-4). Results (1) after 4 weeks of exercise intervention, the symptoms of diabetic normoxic exercise group and diabetic hypoxic exercise group were all alleviated compared with diabetic group, and the effect of diabetic hypoxic exercise group was more obvious than that of normal control group. The levels of TG and LDL-C in serum of diabetic rats were significantly higher than those in diabetic group (P0.01). Serum LDL-C and TC were significantly decreased in diabetic normoxic exercise group (P0.01), and serum LDL-CU TC and TG were significantly decreased (P0.01) in diabetic hypoxic exercise group (P0.01), compared with diabetic hypoxic exercise group, LDL-C and TC were significantly increased (P0.05). The serum TC and LDL-C of diabetic rats in normoxic exercise group were higher than those in normal exercise group (P0.05), the TG of diabetic group was significantly higher than that of normal control group (P0.05), and that of diabetic group was significantly higher than that of normal control group (P0.05), and that of diabetic group was significantly higher than that of diabetic group (P0.05). Compared with the diabetic hypoxic exercise group, the diabetic normoxic exercise group had a higher FBG (P0.05). (4) than the normal control group (P0.05), the diabetic exercise group significantly increased fins (P0.05), and compared with the diabetic hypoxic exercise group (P0.01), the diabetic normoxic exercise group was significantly higher than the diabetic hypoxic exercise group (P0.01), and compared with the diabetic hypoxic exercise group, the diabetic normoxic exercise group was significantly higher than the control group (P0.05). Fins in diabetic normoxic exercise group and diabetic hypoxic exercise group was significantly decreased (P0.01), and fins in diabetic normoxic exercise group was significantly higher than that in diabetic hypoxic exercise group (P0.05). (5) compared with normal control group. The contents of InsRGCK in liver and GLUT-4 in visceral adipose tissue in diabetic group were significantly lower than those in diabetic group (P0.01). The contents of InsRGCK and GLUT-4 in liver tissue of diabetic normoxic exercise group and diabetic hypoxic exercise group were significantly higher than those of diabetic hypoxic exercise group (P0.05), and the GCK content of diabetic normoxic exercise group was significantly lower than that of diabetic hypoxic exercise group (P0.05). 4. Conclusion both normoxic and hypoxic exercise can decrease fasting blood glucose and fasting insulin, decrease serum TGTC and LDL-C, increase HDL content, increase the content of InsRGCK in liver and GLUT-4 in visceral adipose tissue. It is beneficial to promote the recovery of glucose and lipid metabolism disorder in type 2 diabetic rats, and the effect of hypoxic exercise intervention is better than that of normoxic exercise.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7
本文编号：2127257
[Abstract]:1. Objective to observe the effects of normoxic and hypoxic exercise on glucose and lipid metabolism in type 2 diabetic rats, and to explore the effect of normoxic and hypoxic exercise on type 2 diabetes mellitus and its possible mechanism. Methods 60 male Sprague-Dawley (SD) rats aged 6 weeks and 8 weeks old were selected as experimental objects, 10 rats were randomly selected as normal control group, and the rest were fed with high sugar and high fat diet for 4 weeks. The rat model of type 2 diabetes was established by intraperitoneal injection of streptozotocin (STZ). Fasting blood glucose (g16.7mmol L-1) was used as the model standard. The successful type 2 diabetic rats were randomly divided into diabetic group, diabetic normoxic exercise group and diabetic hypoxic exercise group with 10 rats in each group. The latter two groups were trained in treadmill training for 4 weeks (slope 0 掳, speed 15m min-1 60 min d-1d W-1), and diabetic hypoxic exercise group exercised in hypoxic tents (simulated altitude 2500m, oxygen concentration 15.0%). After the last exercise, all rats fasted for 12 hours. After anaesthesia, blood, liver and perirenal fat were taken from abdominal cavity. Fasting blood glucose (FBG), fasting insulin (fins), serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL-C), insulin receptor protein (InsR), glucose kinase (GCK) and glucose in visceral adipose tissue were measured in rats. The content of transporter protein (GLUT-4). Results (1) after 4 weeks of exercise intervention, the symptoms of diabetic normoxic exercise group and diabetic hypoxic exercise group were all alleviated compared with diabetic group, and the effect of diabetic hypoxic exercise group was more obvious than that of normal control group. The levels of TG and LDL-C in serum of diabetic rats were significantly higher than those in diabetic group (P0.01). Serum LDL-C and TC were significantly decreased in diabetic normoxic exercise group (P0.01), and serum LDL-CU TC and TG were significantly decreased (P0.01) in diabetic hypoxic exercise group (P0.01), compared with diabetic hypoxic exercise group, LDL-C and TC were significantly increased (P0.05). The serum TC and LDL-C of diabetic rats in normoxic exercise group were higher than those in normal exercise group (P0.05), the TG of diabetic group was significantly higher than that of normal control group (P0.05), and that of diabetic group was significantly higher than that of normal control group (P0.05), and that of diabetic group was significantly higher than that of diabetic group (P0.05). Compared with the diabetic hypoxic exercise group, the diabetic normoxic exercise group had a higher FBG (P0.05). (4) than the normal control group (P0.05), the diabetic exercise group significantly increased fins (P0.05), and compared with the diabetic hypoxic exercise group (P0.01), the diabetic normoxic exercise group was significantly higher than the diabetic hypoxic exercise group (P0.01), and compared with the diabetic hypoxic exercise group, the diabetic normoxic exercise group was significantly higher than the control group (P0.05). Fins in diabetic normoxic exercise group and diabetic hypoxic exercise group was significantly decreased (P0.01), and fins in diabetic normoxic exercise group was significantly higher than that in diabetic hypoxic exercise group (P0.05). (5) compared with normal control group. The contents of InsRGCK in liver and GLUT-4 in visceral adipose tissue in diabetic group were significantly lower than those in diabetic group (P0.01). The contents of InsRGCK and GLUT-4 in liver tissue of diabetic normoxic exercise group and diabetic hypoxic exercise group were significantly higher than those of diabetic hypoxic exercise group (P0.05), and the GCK content of diabetic normoxic exercise group was significantly lower than that of diabetic hypoxic exercise group (P0.05). 4. Conclusion both normoxic and hypoxic exercise can decrease fasting blood glucose and fasting insulin, decrease serum TGTC and LDL-C, increase HDL content, increase the content of InsRGCK in liver and GLUT-4 in visceral adipose tissue. It is beneficial to promote the recovery of glucose and lipid metabolism disorder in type 2 diabetic rats, and the effect of hypoxic exercise intervention is better than that of normoxic exercise.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7
【参考文献】
相关期刊论文 前10条
1 张荷;周越;张一民;陈圣菊;张俊芬;;低氧运动对肥胖大鼠胰岛素抵抗及血脂代谢的影响[J];北京体育大学学报;2016年09期
2 黄森;刘建红;周志宏;欧明毫;刘晟;王奎;;高住低练对大鼠骨骼肌PI3K/PKB/mTOR信号通路基因表达的影响[J];中国运动医学杂志;2013年02期
3 王宁琦;胡扬;官余凌;赵华;;4周低氧运动结合饮食控制对肥胖青年体重、血脂及胰岛素抵抗的影响[J];中国运动医学杂志;2012年04期
4 潘哲浩;翁锡全;朱宪锋;沈财利;;间歇低氧暴露对高脂膳食大鼠胰岛素敏感性的影响[J];中国运动医学杂志;2012年03期
5 郭吟;陈佩杰;陈文鹤;;4周有氧运动对肥胖儿童青少年身体形态、血脂和血胰岛素的影响[J];中国运动医学杂志;2011年05期
6 钱荣立;;控制糖尿病,刻不容缓——2010联合国糖尿病日:中国蓝光行动[J];中国糖尿病杂志;2010年12期
7 钱荣;谈艳;汪宗宝;叶红;;不同运动强度下糖尿病大鼠骨骼肌胰岛素受体mRNA表达及氧化应激变化[J];第三军医大学学报;2010年09期
8 陈旭健;张原琪;;红菇多糖的提取及其降血糖、血脂作用研究[J];食品科学;2010年09期
9 林文_";李颖;翁锡全;;间歇性低氧和运动对高脂饮食诱导胰岛素抵抗大鼠血清内脂素的影响[J];中国运动医学杂志;2008年02期
10 马延超;张缨;刘花层;;不同低氧训练方式对血脂、体重及其变化机理的研究[J];中国体育科技;2007年05期
相关硕士学位论文 前1条
1 雷雨;低氧及低氧结合运动对肥胖大鼠身体成分的影响及机制研究[D];华南师范大学;2007年
，本文编号：2127257
本文链接：https://www.wllwen.com/jiaoyulunwen/tylw/2127257.html
