急性大强度运动前后人体血液代谢组学特征的研究
发布时间：2018-08-11 19:25
【摘要】：目的:急性大强度运动是一种剧烈运动,会对运动负荷产生氧化应激。本研究以健康成年男性为研究对象,基于核磁共振(NMR)代谢组学方法对受试者进行急性大强度运动后不同时间段的血浆进行代谢组学分析,探讨其代谢特征,旨在明确运动对体内代谢物的影响,发现与运动密切相关的代谢化合物,从代谢物层面为研究运动监控的相关问题提供新思路,为科学安排运动训练提供参考。方法:选取山西大学2014级体育教育专业男生10名(经筛选后保留8人),全力骑行功率自行车30s×3/间歇3min,负荷为0.075Kg/Kg体重。分别于清晨8点安静时、运动后即刻、运动后1小时、运动后1天、运动后1周进行肘静脉采血5mL(除运动后即刻与1小时之外,其余全为清晨空腹采血),标记为E1、E2、E3、E4、E5。采集的血液经离心、去蛋白等处理后进行1H-NMR检测。采用Mest ReNova、SIMCA-P11.0、SPSS17.0软件等进行处理与分析并找出差异代谢标志物。结果:(1)基于NMR代谢组学技术研究,人体在急性大强度运动后即刻与安静状态时代谢物相比代谢物存在显著性差异,本研究共发现13种代谢差异物(VIP1且P0.01):包括亮氨酸、异亮氨酸、缬氨酸、乳酸、谷氨酸、谷氨酰胺、肌酸、肉毒碱、甜菜碱、乙酰乙酸、甘氨酸、甘油、葡萄糖,这几种代谢产物在运动后即刻积分值只有乳酸呈上升趋势,其余均呈下降趋势。(2)运动后1小时与运动前相比的差异代谢标志物共有8种,分别是:亮氨酸、异亮氨酸、缬氨酸、乳酸、甜菜碱、乙酰乙酸、甘油(P0.01)、肌酸(P0.05),这几种代谢产物除乳酸趋势向上外,其余代谢物均依旧趋势向下。运动后1天与运动前相比的差异代谢标志物只有甜菜碱(P0.05)呈下降趋势,运动后1周与运动前相比已无显著性差异。结论:(1)在急性大强度运动干预下,运动后即刻与运动前的差异代谢标志物共有13种,只有乳酸呈上升趋势,约为运动前的3倍,但可恢复。(2)在急性大强度运动干预下,运动后人体无氧代谢供能系统的磷酸原系统、糖酵解系统以及脂肪代谢、氨基酸代谢等过程产生一系列变化,并且这些指标在1周内基本恢复。
[Abstract]:Objective: acute high-intensity exercise is a violent exercise that produces oxidative stress on exercise load. In this study, the plasma metabolites of healthy adult men at different time periods after acute high-intensity exercise were analyzed based on (NMR) metabonomics, and the metabolic characteristics of plasma were investigated. The aim of this paper is to clarify the effect of exercise on metabolites in vivo, to find the metabolites closely related to exercise, to provide a new idea for the study of the related problems of exercise monitoring from the metabolite level, and to provide a reference for the scientific arrangement of exercise training. Methods: a total of 10 male students (8 of whom were retained after selection) were selected from 2014 P. E. major in Shanxi University. The bicycle was cycled for 30 seconds 脳 3 / 3 / interval for 3 mins, and the load was 0.075Kg/Kg weight. 5mL was collected from the elbow vein 1 week after exercise at 8 am at rest, immediately after exercise, 1 hour after exercise and 1 week after exercise (except immediately after exercise and 1 hour after exercise, all of which were collected on an empty stomach in the morning). The collected blood was tested for 1H-NMR after centrifugation, deproteinization and other treatments. SIMCA-P11.0 SPSS 17.0 software was used to process and analyze the differential metabolic markers. Results: (1) based on the study of NMR metabolomics, there were significant differences in metabolites between human body and those in quiet state immediately after acute intensive exercise. There were 13 metabolites (VIP1 and P0.01): leucine and isoleucine were found in this study. Valine, lactic acid, glutamine, creatine, carnitine, betaine, acetoacetic acid, glycine, glycerol, glucose, these metabolites showed an upward trend at the instant after exercise. (2) there were 8 different metabolic markers (leucine, isoleucine, valine, lactic acid, betaine, acetoacetic acid) between 1 hour after exercise and 1 hour after exercise. Glycerol (P0.01), creatine (P0.05), these metabolites except lactate trend upward, the other metabolites are still downward trend. Only betaine (P0.05) showed a decreasing trend 1 day after exercise compared with before exercise, and there was no significant difference between before exercise and 1 week after exercise. Conclusion: (1) under the intervention of acute high intensity exercise, there were 13 different metabolic markers immediately after exercise and before exercise, only lactic acid showed an upward trend, about 3 times as much as before exercise, but could recover. (2) under the intervention of acute high intensity exercise, there were 13 different metabolic markers. After exercise, a series of changes were found in the prophosphate system, glycolysis system, fat metabolism, amino acid metabolism and so on in the anaerobic energy supply system of human body, and these indexes basically recovered within one week.
【学位授予单位】：山西大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7
本文编号：2178007
[Abstract]:Objective: acute high-intensity exercise is a violent exercise that produces oxidative stress on exercise load. In this study, the plasma metabolites of healthy adult men at different time periods after acute high-intensity exercise were analyzed based on (NMR) metabonomics, and the metabolic characteristics of plasma were investigated. The aim of this paper is to clarify the effect of exercise on metabolites in vivo, to find the metabolites closely related to exercise, to provide a new idea for the study of the related problems of exercise monitoring from the metabolite level, and to provide a reference for the scientific arrangement of exercise training. Methods: a total of 10 male students (8 of whom were retained after selection) were selected from 2014 P. E. major in Shanxi University. The bicycle was cycled for 30 seconds 脳 3 / 3 / interval for 3 mins, and the load was 0.075Kg/Kg weight. 5mL was collected from the elbow vein 1 week after exercise at 8 am at rest, immediately after exercise, 1 hour after exercise and 1 week after exercise (except immediately after exercise and 1 hour after exercise, all of which were collected on an empty stomach in the morning). The collected blood was tested for 1H-NMR after centrifugation, deproteinization and other treatments. SIMCA-P11.0 SPSS 17.0 software was used to process and analyze the differential metabolic markers. Results: (1) based on the study of NMR metabolomics, there were significant differences in metabolites between human body and those in quiet state immediately after acute intensive exercise. There were 13 metabolites (VIP1 and P0.01): leucine and isoleucine were found in this study. Valine, lactic acid, glutamine, creatine, carnitine, betaine, acetoacetic acid, glycine, glycerol, glucose, these metabolites showed an upward trend at the instant after exercise. (2) there were 8 different metabolic markers (leucine, isoleucine, valine, lactic acid, betaine, acetoacetic acid) between 1 hour after exercise and 1 hour after exercise. Glycerol (P0.01), creatine (P0.05), these metabolites except lactate trend upward, the other metabolites are still downward trend. Only betaine (P0.05) showed a decreasing trend 1 day after exercise compared with before exercise, and there was no significant difference between before exercise and 1 week after exercise. Conclusion: (1) under the intervention of acute high intensity exercise, there were 13 different metabolic markers immediately after exercise and before exercise, only lactic acid showed an upward trend, about 3 times as much as before exercise, but could recover. (2) under the intervention of acute high intensity exercise, there were 13 different metabolic markers. After exercise, a series of changes were found in the prophosphate system, glycolysis system, fat metabolism, amino acid metabolism and so on in the anaerobic energy supply system of human body, and these indexes basically recovered within one week.
【学位授予单位】：山西大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7
【参考文献】
相关期刊论文 前10条
1 韩雨梅;郑晓莉;李建英;赵剑峰;向欢;;优秀龙舟运动员尿液~1H NMR代谢组学研究[J];中国运动医学杂志;2015年09期
2 马晓丽;骆新;卢伟;李琳琳;王烨;毛新民;;基于~1H核磁共振谱分析的自发性高血压大鼠尿液代谢特征[J];中国实验动物学报;2014年04期
3 朱珊珊;刘云发;于淼;;Wingate无氧功和变速冲刺无氧功的对比分析[J];辽宁体育科技;2014年04期
4 刘耀荣;徐玄冲;时倩;周里;;短时大强度运动过程中功率衰减与无氧做功分段特征[J];中国运动医学杂志;2014年05期
5 王一民;由文华;熊正英;;支链氨基酸对耐力训练大鼠某些生化指标的影响[J];陕西师范大学学报(自然科学版);2013年06期
6 戴曰梅;;核磁共振技术在生命科学领域的应用[J];兵工自动化;2013年04期
7 左群;王静;于新凯;;离心运动后大鼠血清和骨骼肌甘氨酸、苏氨酸、精氨酸及赖氨酸含量的变化[J];中国运动医学杂志;2013年01期
8 张凯杰;;支链氨基酸与运动性中枢疲劳[J];当代体育科技;2012年28期
9 徐倩;;运动性酮体的研究现状[J];体育科研;2012年05期
10 杨东升;刘晓莉;乔德才;;大鼠运动性疲劳形成和恢复过程ECoG的动态研究[J];体育科学;2012年04期
相关博士学位论文 前1条
1 黄彩华;格斗对抗性项目代谢调节与适应的核磁共振代谢组学研究[D];福建师范大学;2012年
相关硕士学位论文 前1条
1 李秋雨;虾青素干预人体安静时及急性大强度运动后血清~1H-NMR代谢组学研究[D];山西大学;2016年
，本文编号：2178007
本文链接：https://www.wllwen.com/jiaoyulunwen/tylw/2178007.html
