肌酸补充对葡萄糖剥夺引起的BKca电流变化和胞内游离钙离子浓度的影响
发布时间：2018-10-19 15:01
【摘要】：目的:大电导钙激活钾通道(BK_(Ca))对神经元兴奋性以及动作电位发放频率具有重要调控作用,拟观察肌酸补充对皮层神经元葡萄糖剥夺时BK_(Ca)电流密度及胞内钙离子浓度的影响。方法:全细胞膜片钳技术记录肌酸干预对不同时长葡萄糖剥夺原代培养大鼠皮层神经元BK_(Ca)电流的变化;应用激光共聚焦检测神经元胞内游离钙离子浓度。结果:1)葡萄糖剥夺1~2 h BK_(Ca)电流密度显著增加(P0.01),4~5 h显著减小(P0.01);胞内游离Ca~(2+)荧光强度随葡萄糖剥夺1~5 h逐渐增强(P0.01)。2)肌酸孵育显著减小原代培养神经元BK_(Ca)电流密度以及胞内游离Ca~(2+)荧光强度(P0.01)。结论:葡萄糖剥夺1~2 h使胞内游离Ca2+浓度显著升高,激活BK_(Ca)大量开放,使神经元超极化,降低神经元兴奋性;Cr可有效抑制葡萄糖剥夺引起BK_(Ca)电流增加以及胞内钙离子浓度升高,维持锥体神经元的兴奋性。
[Abstract]:Aim: to investigate the effects of creatine supplementation on BK_ (Ca) current density and intracellular calcium concentration in cortical neurons after glucose deprivation. Methods: whole-cell patch clamp technique was used to record the changes of BK_ (Ca) currents in primary cultured rat cortical neurons exposed to glucose deprivation for different periods, and the intracellular free calcium concentration of neurons was detected by confocal laser. Results: 1) the current density of BK_ (Ca) increased significantly (P0.01) at 1h of glucose deprivation and decreased significantly at 4h (P0.01), and the fluorescence intensity of intracellular free Ca~ (2) increased gradually with 1h of glucose deprivation (P0.01). 2) the current density of BK_ (Ca) in primary cultured neurons was significantly decreased by creatine incubation. And intracellular free Ca~ (2) fluorescence intensity (P0.01). Conclusion: 1h glucose deprivation can significantly increase the concentration of intracellular free Ca2, activate BK_ (Ca), hyperpolarization and decrease the excitability of neurons, Cr can effectively inhibit the increase of BK_ (Ca) current and the increase of intracellular Ca ~ (2 +) concentration induced by glucose deprivation. Maintain excitability of pyramidal neurons.
【作者单位】： 北京体育大学运动人体科学学院;北京体育大学教育部运动与体质健康重点实验室;
【基金】：国家自然科学基金资助项目(编号:31271278) 中央高校基本科研业务费专项资金资助项目(编号:2016RC004)
【分类号】：G804.7
，
本文编号：2281488
[Abstract]:Aim: to investigate the effects of creatine supplementation on BK_ (Ca) current density and intracellular calcium concentration in cortical neurons after glucose deprivation. Methods: whole-cell patch clamp technique was used to record the changes of BK_ (Ca) currents in primary cultured rat cortical neurons exposed to glucose deprivation for different periods, and the intracellular free calcium concentration of neurons was detected by confocal laser. Results: 1) the current density of BK_ (Ca) increased significantly (P0.01) at 1h of glucose deprivation and decreased significantly at 4h (P0.01), and the fluorescence intensity of intracellular free Ca~ (2) increased gradually with 1h of glucose deprivation (P0.01). 2) the current density of BK_ (Ca) in primary cultured neurons was significantly decreased by creatine incubation. And intracellular free Ca~ (2) fluorescence intensity (P0.01). Conclusion: 1h glucose deprivation can significantly increase the concentration of intracellular free Ca2, activate BK_ (Ca), hyperpolarization and decrease the excitability of neurons, Cr can effectively inhibit the increase of BK_ (Ca) current and the increase of intracellular Ca ~ (2 +) concentration induced by glucose deprivation. Maintain excitability of pyramidal neurons.
【作者单位】： 北京体育大学运动人体科学学院;北京体育大学教育部运动与体质健康重点实验室;
【基金】：国家自然科学基金资助项目(编号:31271278) 中央高校基本科研业务费专项资金资助项目(编号:2016RC004)
【分类号】：G804.7
，
本文编号：2281488
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