甲烷水对一次性力竭运动所致大鼠心肌损伤的保护作用

发布时间：2019-04-04 15:55

【摘要】：目的:观察灌服甲烷水对一次性力竭运动所致大鼠心肌缺血性损伤、心脏组织内氧化应激和炎症因子的影响,探讨其对运动性心肌损伤产生保护作用的可能机制。方法:24只雄性SD大鼠随机分为安静对照组(C组)、运动对照组(EP组)、运动甲烷水组(EM组)。EP组和EM组分别于运动前30 min灌服蒸馏水和甲烷水,之后进行一次性力竭跑台运动。采用化学比色法、ELISA和Luminex x MAP溲技术(液态悬浮芯片技术)检测大鼠血清和心肌中生化指标。结果:1)EM组较EP组运动时间显著延长(P0.01)。2)与C组比较,EP组大鼠血清中IL-1β、IL-6含量极显著升高(P0.01),心肌e NOS水平显著降低,MDA含量显著升高(P0.05),3-NT含量极显著性升高(P0.01);与EP组比较,EM组血清中c Tn I水平显著上升,IL-1β、IL-6含量显著下降(P0.05),大鼠心肌e NOS水平显著升高(P0.05),SOD活性、T-AOC含量极显著上升(P0.01),MDA含量、3-NT含量显著降低(P0.05)。结论:灌服甲烷水提高大鼠运动能力,可能是通过增加心肌e NOS水平,催化NO生成,舒张血管;提高心肌抗氧化能力,改善大鼠心肌氧化应激损伤,从而对运动性心肌损伤具有一定的保护作用。
[Abstract]:Aim: to observe the effects of methane water on myocardial ischemic injury, oxidative stress and inflammatory factors induced by exhaustive exercise in rats, and to explore the possible mechanism of its protective effect on exercise-induced myocardial injury. Methods: 24 male SD rats were randomly divided into three groups: quiet control group (C group), exercise control group (EP group), exercise methane water group (EM group). EP group and EM group) 30 min before exercise with distilled water and methane water, respectively. After that, a one-time exhaustive run-off exercise was carried out. Biochemical indexes in serum and myocardium of rats were detected by chemical colorimetry, ELISA and Luminex x MAP technique (liquid suspension chip technique). Results: 1) the exercise time of EM group was significantly longer than that of EP group (P0.01). 2) compared with C group, the levels of IL-1 尾 and IL-6 in serum of EP group increased significantly (P0.01), and the level of myocardial e-NOS decreased significantly. The content of MDA was significantly higher (P0.05) and the content of 3-NT was significantly higher (P0.01). Compared with EP group, serum c Tn I level in EM group increased significantly, IL-1 尾 and IL-6 contents decreased significantly (P0.05), and myocardial e-NOS level increased significantly (P0.05), SOD activity). T-AOC content increased significantly (P0.01), MDA content, 3-NT content decreased significantly (P0.05). Conclusion: taking methane water to improve the exercise ability of rats may be by increasing the level of myocardial e-NOS, catalyzing the formation of NO and relaxing the blood vessels. It can improve the anti-oxidation ability of myocardium and improve the oxidative stress injury of rat heart muscle, so it has a certain protective effect on exercise-induced myocardial injury.
【作者单位】：上海体育学院;
【基金】：上海体育学院研究生教育创新计划(编号:yjscx2016018) 上海市人类运动能力开发与保障重点实验室(上海体育学院)项目(编号:11DZ2261100)
【分类号】：G804.2

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