多糖基生物材料制备核壳纳米粒及其对药物的pH敏感释放研究

发布时间：2018-04-03 20:06

本文选题：核壳结构　切入点：层层自组装　出处：《天津大学》2015年硕士论文

【摘要】：目前,由于具备在不同环境刺激下对抗癌药物,标记染料的控制释放,以及其本身固有的生物相容性,基于多糖类材料制备的多功能纳米粒备受研究者青睐。本文主要利用壳聚糖(Cs)和聚乙烯醇磷酸酯(PPVA)作为静电多层材料,借助层层自组装技术,交替沉积在3-氨丙基三乙氧基硅烷改性修饰的二氧化硅球表面制备了多功能纳米粒。通过透射电子显微镜分析和粒径分布表征,证明了纳米粒具备良好的球形形貌。7-羟基香豆素和罗丹明B作为二种模拟药物分别装载在了核层和壳层之中。通过激光共聚焦显微镜证明了核壳结构,进一步验证了药物的分布位置。通过研究不同pH环境下,7-羟基香豆素和罗丹明B的共释放行为,表明了SiO2(PPVA/Cs)n这种多功能核壳纳米粒在药物递送领域具有崭新的应用前景。另一方面,我们还利用预凝胶方法制备了多糖基(果胶,卡拉胶)羟基磷灰石的核壳纳米粒。通过固定多糖结构单元与钙离子摩尔比,调节羟基磷灰石的钙磷比,研究了多糖分子与羟基磷灰石的相互作用,通过透射电镜观察了纳米粒的形貌特征,最终确定了以羟基磷灰石晶体为核,阴离子多糖为壳的无机-有机杂化核-壳纳米粒的制备方法。通过在纳米粒表面进行静电层层自组装得到了核壳纳米杂化材料,材料不仅显示出了果胶和卡拉胶的生物相容性,而且具备羟基磷灰石的pH可降解性。最后,通过研究两种纳米粒在不同pH下对罗丹明6G的释放行为,得出结论：在酸性条件下由于羟基磷灰石的溶解导致水溶性药物罗丹明6G从纳米粒中大量释放。研究结果表明,基于生物多糖形成的纳米核壳材料在生物医药领域具有潜在的研究价值。
[Abstract]:At present, due to the ability in different environment stimulation on anticancer drug controlled release of dye, and its inherent biocompatibility, multifunctional nanoparticles polysaccharide material preparation has attracted great attention from researchers. This paper mainly based on the use of chitosan and polyvinyl alcohol (Cs) phosphate (PPVA) as an electrostatic multilayer material with layers the self-assembly technology, multifunctional nanoparticles prepared by alternating silica spheres surface were deposited on 3- aminopropyltriethoxysilane modified. By transmission electron microscopy analysis and characterization of particle size distribution, proved that the nanoparticles have good spherical morphology of.7- hydroxycoumarin and Rhodamine B as two kinds of drugs were loaded in the nuclear simulation layer and shell. Using laser scanning confocal microscopy proved that the core-shell structure, further verify the location of drugs. Through the study of different pH environment, 7- hydroxy aromatic Legumin and CO release behavior of rhodamine B, showed that SiO2 (PPVA/Cs) n this multifunctional core shell nanoparticles in drug delivery field has new application prospects. On the other hand, we also use the pre gel method based on polysaccharide preparation (pectin, carrageenan) core-shell nanoparticles of hydroxyapatite. By fixing the polysaccharide the structure unit and the calcium ion molar ratio of calcium and phosphorus regulated hydroxyapatite ratio, interaction of polysaccharide molecules and hydroxyapatite, the morphology of nanoparticles was observed by transmission electron microscopy, and finally determines the hydroxyapatite crystal nucleus, anionic polysaccharide to a preparation method of shell organic-inorganic hybrid core-shell nanoparticles by. Electrostatic self-assembly in the surface of the nanoparticles obtained core-shell nano hybrid materials, materials not only shows the compatibility of pectin and carrageenan organisms, but with hydroxyapatite pH Degradation. Finally, through the study of two kinds of nanoparticles in different pH on Luo Danming 6G's release behavior, draw a conclusion: under acidic conditions due to hydroxyapatite dissolution causes the water soluble drug from Luo Danming 6G to release large amounts of nanoparticles. The results show that the nano core-shell materials biological polysaccharide formation have a potential value in based on the field of biology and medicine.

【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TQ460.4

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