CuO修饰的碳纳米平台用于多重成像和联合增强的抗肿瘤治疗（英文）

发布时间：2024-11-02 14:20

　　 将多功能纳米平台的设计合成应用于肿瘤的联合治疗得到研究人员的广泛关注.本研究通过水热法制备了形貌均匀的光热材料碳纳米球,表面负载CuO和抗癌药盐酸阿霉素(DOX)实现光热/化学动力/化疗联合治疗. CuO通过静电吸附负载在碳纳米球(CNSs)表面,电子在C-2p与Cu-3d之间的跃迁提高了材料的光热转换效率. CuO也可以作为化学动力试剂,在肿瘤部位释放Cu2+并通过Haber-Weiss和类Fenton反应产生羟基自由基诱导肿瘤细胞凋亡. DOX吸附在CuO@CNSs表面,表现出p H响应释放和近红外激光刺激响应的释放效果.研究结果表明, CuO@CNSs-DOX纳米平台在体内外都有很好的抗癌效果,在肿瘤治疗方面有很大的应用潜力.

【文章页数】：25 页

【文章目录】：
INTRODUCTION
EXPERIMENTAL SECTION
    Preparation of CNSs and CuO@CNSs
    The release of Cu2+from CuO@CNSs
    Extracellular·OH detection
    Intracellular·OH detection
    Photothermal properties
    Photothermal conversion efficiency
    Live/dead cell staining
    Computational details
    Loading of DOX
    Release behavior of DOX
    Cellular uptake
    Biocompatibility of CuO@CNSs
    In vitro cytotoxicity detection
    Flow cytometry
    Animal experiments
    IR and PA imaging
    Anti-tumor experiment
    Biocompatibility in vivo
    Statistical analysis
RESULTS AND DISCUSSION
    Fabrication and characterization
    The pH-sensitive release of Cu2+and production of·OH
    Photothermal effects of CuO@CNSs
    DOX loading and releasing behavior
    Celluar uptake assays
    In vitro cytotoxicity of CuO@CNSs
    IR and PA bioimaging
    In vivo antitumor efficacy
CONCLUSIONS
    Experimental Chemicals
    Characteristic
    Cell culture and animal models
    Statistical Analysis



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