拉曼-荧光联合光谱水下原位探测技术研究

发布时间：2018-04-27 21:04

本文选题：拉曼 + 荧光　；参考：《光谱学与光谱分析》2017年10期

【摘要】：深海热液环境中存在着巨大的化学和热梯度,快速剧烈的混合和生物过程产生了多种多样的矿物过程,并培养了大量的化学合成微生物。激光拉曼光谱非常适合于深海热液环境矿物过程的探测,然而要对矿物与微生物作用过程进行研究,还需要与荧光光谱技术进行联合,针对此需求开展了原理验证实验研究。在实验室搭建了一套拉曼-荧光联合光谱探测桌面系统,利用一台双波长激光器同时作为拉曼光谱和荧光光谱的激发光源,其中拉曼光谱采用532nm波长,荧光光谱采用266nm波长,双波长激光器发出的光束经分光镜分为两路,经过后向散射光路收集的两路信号分别进入两个小型光纤光谱仪进行分光探测,拉曼光谱采用QE65000光谱仪,荧光光谱采用USB2000光谱仪,通过软件可以方便的分别设置两个光谱仪的参数。利用搭建的实验系统对海水和拟菱形藻样品进行探测,分别获得了海水样品的SO2-4拉曼光谱和可溶性有机物(CDOM)荧光光谱,拟菱形藻样品的类胡萝卜素拉曼光谱和类蛋白、叶绿素等荧光光谱,实验结果证明了研制小型拉曼-荧光联合光谱探测装置的可行性,并为发展原位联合光谱探测装置提供了技术参考。
[Abstract]:There are huge chemical and thermal gradients in deep-sea hydrothermal environment. The rapid and violent mixing and biological processes produce a variety of mineral processes and a large number of chemosynthetic microorganisms have been cultured. Laser Raman spectroscopy is very suitable for the detection of mineral processes in deep-sea hydrothermal environment. However, in order to study the interaction between minerals and microorganisms, it is necessary to combine with fluorescence spectroscopy. A set of Raman fluorescence detection desktop system was set up in the laboratory. A dual wavelength laser was used as the excitation source of Raman spectrum and fluorescence spectrum. The Raman spectrum used 532nm wavelength and the fluorescence spectrum used 266nm wavelength. The beams from the dual-wavelength laser are divided into two channels through the spectroscope, and the two signals collected through the backscattering light path enter into two small optical fiber spectrometers respectively for spectroscopic detection. The Raman spectrum is QE65000 spectrometer, and the fluorescence spectrum is USB2000 spectrometer. The parameters of the two spectrometers can be easily set by software. The SO2-4 Raman spectra and soluble organic compounds fluorescence spectra, carotenoid Raman spectra and protein-like spectra of sea water samples were obtained by using the experimental system to detect sea water and diamond-like alga, respectively, and the results were as follows: (1) the SO2-4 Raman spectra of sea water samples and the fluorescence spectra of soluble organic compounds were obtained, and the carotenoid Raman spectra and protein like spectra were obtained. The experimental results show that it is feasible to develop a small Raman fluorescence combined spectrometer and provide a technical reference for the development of in situ joint spectroscopic detector.
【作者单位】：中国海洋大学信息科学与工程学院;
【基金】：国家自然科学基金项目(61575181) 国家(863计划)项目(2012AA09A405)资助
【分类号】：O657.3;P714

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