碳源和盐度对好氧反硝化细菌脱氮特性的影响

发布时间：2017-12-27 02:12

本文关键词：碳源和盐度对好氧反硝化细菌脱氮特性的影响　出处：《华南师范大学学报(自然科学版)》2016年06期 　论文类型：期刊论文

【摘要】：采用16S rDNA序列分析对菌株LZX301进行了初步鉴定,在150 r/m摇瓶好氧培养,探讨了碳源及盐度对菌株好氧反硝化特性的影响.结果表明,该菌株16S rDNA序列与Pseudomonas stutzeri ATTC 17594(AY905607.1)等3株施氏假单胞菌序列相似度为99%,系统发育树分析显示菌株LZX301与P.stutzeri的关系比同属的P.aeruginosa和P.putida更近,因此初步确定菌株LZX301为P.stutzeri.培养液初始含7 mg/L亚硝酸盐和28 mg/L硝酸盐、C/N比为10∶1条件下,以葡萄糖、乙酸钠和蔗糖为碳源时无机氮去除率分别为79.1%、67.9%和38.8%,氨氮积累量分别为1.978、1.224、0.727 mg/L.以葡萄糖为唯一碳源时,在5‰、15‰、25‰等3个盐度下无机氮总去除率分别为73.2%、85.8%和78.7%,其中硝酸盐去除率分别为89.8%、86.1%和76.5%,亚硝酸盐去除率分别为36.2%、94.7%和96.4%,氨氮质量浓度分别为2.117、0.691、0.595 mg/L.研究结果表明菌株LZX301在盐度5‰~25‰范围内具有较强的好氧反硝化能力,以葡萄糖为碳源脱氮效果最好,对该菌株的应用具有指导意义.
[Abstract]:Using 16S rDNA sequence analysis, strain LZX301 was identified in 150, r/m in shake flask aerobic culture, to investigate the effect of carbon source and salinity on strains of aerobic denitrification characteristics. The results showed that the strain 16S rDNA sequence and Pseudomonas stutzeri ATTC 17594 (AY905607.1) of 3 strains of Pseudomonas stutzeri sequence similarity 99%, phylogenetic tree analysis showed that the relationship between LZX301 and P.stutzeri strains belong to P.aeruginosa and P.putida than the more recent, so we initially identified LZX301 solution containing 7 mg/L 28 mg/L of nitrate, nitrite and C/N ratio of 10: 1 for P.stutzeri. culture conditions, glucose, sodium acetate and sucrose as the carbon source of inorganic nitrogen the removal rates were 79.1%, 67.9% and 38.8%, the ammonia nitrogen accumulation were 1.978, 1.224 and 0.727 mg/L. with glucose as the sole carbon source. In 5, 15, 25 per thousand per thousand per thousand and 3 of total inorganic nitrogen removal under salinity Rates were 73.2%, 85.8% and 78.7%, the nitrate removal rate were 89.8%, 86.1% and 76.5%, the nitrite removal rates were 36.2%, 94.7% and 96.4%, the concentration of ammonia nitrogen were 2.117, 0.691 and 0.595 mg/L. the results showed that strain LZX301 in the salinity of 5 ~25%% range of aerobic denitrification ability strong, the best carbon source denitrification effect on glucose, provide guidance for the application of this strain.
【作者单位】：华南师范大学生命科学学院;广东水产健康安全养殖重点实验室;广东普通高校生态与环境科学重点实验室;清远市水产研究所;
【基金】：国家自然科学基金项目(31472302,31172432) 广东省海洋渔业科技与产业发展专项(A201401B01) 广东省海洋渔业科技推广专项(A201001H02)
【分类号】：X172
【正文快照】： strain LZX301 in practical applications.日益严重的氮污染是最重要的环境问题之一,反硝化作为主要的脱氮途径长期以来被深入研究.传统上认为在厌氧或缺氧条件下反硝化才会发生,ROBERTSON等[1]首次发现具有好氧反硝化能力的Thiosphaera pantotropha改变了这一认识.好氧反硝

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