重铬酸钾对泥鳅鳍细胞系的毒理学研究

发布时间：2018-01-12 22:33

本文关键词：重铬酸钾对泥鳅鳍细胞系的毒理学研究　出处：《大连海洋大学学报》2016年06期 　论文类型：期刊论文

【摘要】：为探究重铬酸钾的毒性机制,建立适合监测铬污染的体外检测系统,以体外培养的泥鳅Misgurnus anguillicaudatus鳍细胞系(DIMF)为试验材料,研究了重铬酸钾的毒性效应。结果表明:通过噻唑蓝(MTT)比色法测定重铬酸钾染毒后细胞的24 h半致死浓度为(25.3±1.2)μmol/L;暴露在浓度为0~30μmol/L的重铬酸钾中,细胞超氧化物歧化酶(SOD)活性随着染毒浓度的增加而增大;谷胱甘肽超氧化物酶(GSH-Px)在重铬酸钾浓度为0~20μmol/L时活性升高,当染毒浓度为30 mol/L时,活力开始下降;谷胱甘肽S-转移酶(GST)活性则随重铬酸钾浓度的升高而降低;微核试验显示,DIMF微核率随染毒浓度的增加呈先升高后降低的趋势,其中最大微核率为0.733%;实时定量PCR结果显示,对照组的金属硫蛋白(MT)基因表达量很低,经重铬酸钾诱导后MT基因表达量显著升高(P0.01)。研究表明,重铬酸钾可对细胞的酶系统和遗传物质造成一定的损伤。
[Abstract]:To explore mechanism of toxicity of potassium dichromate, established in vitro detection system for monitoring of chromium pollution, Misgurnus anguillicaudatus loach fin cell line cultured in vitro (DIMF) as test materials, studied the toxic effects of potassium dichromate. The results showed that the methyl thiazolyl tetrazolium (MTT) assay of 24 cells were exposed to potassium dichromate colorimetric h half lethal concentration method (25.3 + 1.2) mol/L; exposure at the concentration of 0~30 mol/L potassium dichromate, cell superoxide dismutase (SOD) activity increased with increasing concentrations of glutathione; superoxide dismutase (GSH-Px) in potassium concentration is 0~20 ~ mol/L activity increased, when the exposure concentration is 30 mol/L, activity began to decline; glutathione S- transferase (GST) activity increased with the potassium concentration decreased; micronucleus test showed that the micronucleus rate of DIMF first increased and then decreased with the increase of the concentration of exposure, One of the biggest micronucleus rate of 0.733%; real time quantitative PCR results showed that the control group of metallothionein (MT) gene expression level is low, the amount of MT gene expression induced by potassium dichromate was significantly increased (P0.01). The results show that potassium dichromate can be enzyme system of the cell and genetic material to cause some damage.

【作者单位】：大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室;大连海洋大学农业部北方海水增养殖重点实验室;
【基金】：国家自然科学基金资助项目(31272650)
【分类号】：X171.5
【正文快照】： 近年来,电镀、染印等工业中的铬污染对水体的严重危害已引起人们的广泛关注。铬的常见形态是三价铬(Cr3+)和六价铬(Cr6+),其中Cr3+是人和动物的必需微量元素,因此,铬在毒理学研究中主要是指六价铬,6+会造成消化道、呼吸道病变,皮肤过敏,恶性肿瘤等[U,故探讨}a对细胞的毒性效

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