固定化酵母细胞载体制备及去除废水中苯酚效果研究

发布时间：2018-01-15 18:03

  本文关键词：固定化酵母细胞载体制备及去除废水中苯酚效果研究　出处：《西南交通大学》2015年硕士论文　论文类型：学位论文

  更多相关文章： 固定化 载体制备 酵母工程菌 苯酚 去除率

【摘要】：通过显微镜观察海藻酸钠、聚乙烯醇及海藻酸钠和聚乙烯醇混合材料的表观特征,确定了以海藻酸钠和聚乙烯醇为包埋材料,制备固定化酵母菌和固定化酵母工程菌的方法。根据固定化材料包埋条件标准(传质性能、机械强度、降酚能力、重复使用性),确定了最优包埋条件：聚乙烯醇5%、海藻酸钠3%。以废水中苯酚初始浓度、环境温度以及废水pH为控制因子,开展了大量不同环境条件下固定化酵母菌及酵母工程菌降解废水中苯酚效果的试验研究。结果表明。固定化酵母工程菌随着初始浓度、环境温度以及pH值的增大,废水中苯酚去除率均呈现先增大后减小的规律,这说明,对于各个控制因子,固定化酵母工程菌对于废水中苯酚的去除均存在一个最大活性条件。基于试验结果分析,我们得出,固定化酵母工程菌在处理模拟废水中苯酚去除率最快最大时,pH为6,温度为25℃,浓度为1800mg/L。并且模拟出最优条件下,固定化酵母工程菌降解废水中苯酚去除率随时间呈指数规律增长。开展了不同苯酚初始浓度、不同环境温度、以及不同废水pH值条件下,固定化酵母工程菌和固定化酵母菌降去除水中苯酚的去除率的对比分析。得出结论,固定化酵母工程菌对于废水中苯酚的去除率均大于相同条件下固定化酵母菌降解废水中苯酚的去除率。并且当条件发生变化时,固定化酵母工程菌具有更强的适应性。对苯酚降解动力学进行试验,通过废水中苯酚比去除率计算及分析,发现固定化酵母工程菌降解苯酚的过程符合Haldane模型。基于Haldane模型,通过拟合分析,得到了苯酚降解动力学方程,并利用不同工况数据对动力学方程的普遍适用性进行了反演。以上研究结果对于废水中苯酚去除的菌种选择,以及固定化酵母工程菌实际应用于废水中苯酚去除的环境因子控制和不同去除时间后的废水中残留苯酚浓度的预测具有一定的参考意义。
[Abstract]:The apparent characteristics of sodium alginate, polyvinyl alcohol and the mixture of sodium alginate and polyvinyl alcohol were observed under microscope, and the embedding materials of sodium alginate and polyvinyl alcohol were determined. Methods of preparing immobilized yeast and immobilized yeast engineering bacteria. According to immobilized material embedding conditions (mass transfer property mechanical strength phenol reducing ability reusability). The optimal embedding conditions were determined as follows: polyvinyl alcohol 5 and sodium alginate 3. The initial concentration of phenol in wastewater, environmental temperature and pH of wastewater were taken as control factors. The effect of immobilized yeast and yeast engineering bacteria on the degradation of phenol in wastewater under different environmental conditions was studied. The results showed that the immobilized yeast engineering bacteria were treated with the initial concentration. The removal rate of phenol in wastewater increased first and then decreased with the increase of environmental temperature and pH value. There is a maximum activity condition for phenol removal from wastewater by immobilized yeast engineering bacteria. The best removal rate of phenol in immobilized yeast engineering bacteria was pH 6, temperature 25 鈩，

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