有机碳源对SNAD工艺脱氮性能及微生物种群结构的影响

发布时间：2018-01-24 09:55

本文关键词： 同步亚硝化厌氧氨氧化和反硝化有机物生活污水聚合酶链式反应-变性梯度凝胶电泳微生物　出处：《哈尔滨工业大学学报》2016年02期 　论文类型：期刊论文

【摘要】：为考察不同有机碳源质量浓度对亚硝化的全程自养脱氮工艺(SNAD)脱氮性能的影响,将该工艺应用到生活污水的处理中,采用MBR反应器,以葡萄糖作为有机物来源,通过逐步增大COD来实现,并运用PCR-DGGE技术研究了微生物种群结构的变化.反应器运行结果和DGGE图谱分析表明:碳氮比为0~2时,COD的增加不会抑制AOB和Anammox菌,AOB和Anammox菌的菌属种类不受影响,反而通过反硝化作用提高氮去除负荷.总氮去除率和氮去除负荷分别为67%和0.34 kg/(m3·d)左右.碳氮比为3~4及生活污水运行条件下,Anammox菌不受影响,AOB的活性受到抑制,菌属种类减少,脱氮效率下降.生活污水运行阶段,总氮去除率和氮去除负荷平均分别为73%和0.17 kg/(m3·d).Nitrosomonas和Candidatus Kuenenia stuttgartiensis一直是反应器内的优势菌属,共同完成脱氮过程.
[Abstract]:In order to investigate the effect of different organic carbon source mass concentration on the denitrification performance of the whole process of nitrification, the process was applied to the treatment of domestic sewage and MBR reactor was used. Glucose was used as organic source, which was realized by increasing COD step by step. PCR-DGGE technique was used to study the change of microbial population structure. The results of reactor operation and DGGE analysis showed that the ratio of carbon to nitrogen was 0 ~ 2:00. The increase of COD did not inhibit the growth of AOB and Anammox. The species of AOB and Anammox were not affected. On the contrary, the nitrogen removal load was increased by denitrification. The total nitrogen removal rate and nitrogen removal load were about 67% and 0.34 kg/(m3 路d, respectively. The activity of Anammox bacteria was not affected, the species of Anammox were decreased, the nitrogen removal efficiency was decreased, and the operation stage of domestic sewage. The average nitrogen removal efficiency and nitrogen removal load were 73% and 0.17 kg/(m3 路d, respectively. Nitrosomonas and Candidatus Kuenenia stuttgartiensis are the dominant bacteria in the reactor. The denitrification process was completed together.
【作者单位】：水质科学与水环境恢复工程北京市重点实验室(北京工业大学);郑州轻工业学院环境污染治理与生态修复河南省协同创新中心;城市水资源与水环境国家重点实验室(哈尔滨工业大学);
【基金】：国家自然科学基金(51222807) 国家科技重大专项-水专项(2012ZX07202-005)
【分类号】：X703;X172
【正文快照】： 目前,水体中氮素污染物的去除仍是水处理领域研究的热点与难点.传统硝化-反硝化脱氮工艺虽然脱氮效率高,但投资与运行费用高,耗能大,污泥产量高[1].基于亚硝化的全程自养脱氮(CANON)[2]工艺有效地克服了传统脱氮工艺中固有的缺点.CANON工艺是指氨氧化菌(AOB)和厌氧氨氧化(Anam

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