基于鳃的microRNA转录组研究中国蛤蜊对重金属镉的响应

发布时间：2018-02-20 07:45

本文关键词： 中国蛤蜊镉 microRNA 差异表达功能基因　出处：《海洋学报》2016年12期 　论文类型：期刊论文

【摘要】：中国蛤蜊(Mactra chinensis)是一种重要的经济贝类,分布于我国的辽宁和山东。近年来,生境恶化和过度捕捞导致我国蛤蜊资源量减少。我们利用Illumina Hiseq-2500平台对中国蛤蜊鳃的microRNA转录组进行测序,运用差异表达寻找对镉刺激有响应的功能microRNA。结果显示对照组获得了14 415 256条clean reads,实验组获得了15 570 111条clean reads。在这些reads中一共存在14 584 077小RNA,包括1 898 035条unique小RNA,其中对照组和实验组共有187 859条unique小RNA。两个组的小RNA文库的片段长度分布是相似的,大部分小RNA长度分布在26~27nt。在对照组文库中最丰富的片段长度是27nt,其次是28nt、26nt和23nt。在实验组文库中,数量最丰富的片段长度是26nt,其次是27nt、28nt和23nt。经过对序列前体以及结构特征的分析,发现这两个组中一共有50个microRNA,其中已知的是38个,新发现的是12个。对照组和实验组microRNA含量最丰富的片段长度是23nt。通过差异表达分析,5个microRNA基因具有显著性差异且从对照组到实验组的表达量是上调的,其余45个没有显著性的差异。把这50个序列与中国蛤蜊转录组进行比对,一共找到了542个靶基因。5个具有表达差异的基因一共比对到11个靶基因。把这11个靶基因与NCBI数据库比对,4个靶基因在COG中得到注释,1个靶基因在GO中得到注释,6个靶基因在KEGG中得到注释,11个靶基因在nr数据库中得到注释,注释到的基因有泛素蛋白连接酶E3,Wnt信号通路,G蛋白信号调控等。
[Abstract]:Mactra chinensis) is a kind of important economic shellfish, distributed in Liaoning and Shandong provinces of China in recent years. Habitat deterioration and overfishing resulted in the reduction of clam resources in China. We sequenced the microRNA transcriptome in the Gill of Chinese clam using Illumina Hiseq-2500 platform. The results showed that the control group obtained 14,415,256 clean readsand the experimental group obtained 15,570,111 clean reads.There were 14 584,077 small RNAs in these reads, including 1 898,035 unique small RNAs. There were 187,859 unique small RNAs in the control group and the experimental group. The fragment length distribution of the small RNA library in the two groups was similar. The most abundant fragment length in the control library was 27nt, followed by 28ntnt 26nt and 23nt. in the experimental group, the most abundant fragment length was 26nt, and the second was 27ntNt 28nt and 23nt.The sequence precursors and structural characteristics were analyzed. We found that there were 50 microRNAs in these two groups, 38 of which were known. The most abundant fragment length of microRNA in control group and experimental group was 23nt.The expression of 5 microRNA genes was significantly different and up-regulated from control group to experimental group by differential expression analysis. There was no significant difference in the remaining 45 sequences. The 50 sequences were compared with the Chinese clam transcriptome. A total of 542 target genes were found. Five differentially expressed genes were compared to 11 target genes. Comparing these 11 target genes with NCBI database, 4 target genes were annotated in COG and 1 target gene was annotated in go. , 6 target genes were annotated in KEGG, 11 target genes were annotated in nr database, The explained genes include Ubiquitin protein ligase E3 Wnt signaling pathway, G protein signal regulation and so on.
【作者单位】：国家海洋环境监测中心海洋生态室;大连海洋大学水产与生命学院;
【基金】：国家自然科学基金(31572595) 国家海洋局近岸海域生态环境重点实验室开放基金(201511)
【分类号】：X171.5;S917.4

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