柠檬酸废水厌氧颗粒污泥微生物菌群结构解析

发布时间：2018-03-02 02:25

本文关键词： 厌氧颗粒污泥微生物菌群高通量测序柠檬酸废水　出处：《哈尔滨工业大学学报》2016年08期 　论文类型：期刊论文

【摘要】：为揭示柠檬酸废水生物处理过程中功能菌群作用机制,以柠檬酸工业废水内循环厌氧反应塔(IC)中厌氧颗粒污泥为研究对象,统计颗粒粒径分布,通过环境扫描电子显微镜(ESEM)观察颗粒微观形态结构,利用高通量测序技术分析微生物多样性及菌群特征.结果发现,粒径在1.0~4.0 mm的颗粒所占比例最多,为74.4%.ESEM显示微生物分布以球形细菌为主.高通量测序得到8 397条有效序列,可划分操作分类单元(OTU)873个,Alpha多样性指数显示样品文库覆盖率0.936,Shannon指数为4.376,而ACE指数与Chao1指数分别为3 415.51与2 246.51,反映颗粒污泥中微生物种类与数量均较多.微生物菌群主要包括4大类,分别为可降解有机物的水解发酵菌群Paludibacter、Parabacteroides、Erysipelotrichaceae、Clostridium、Phascolarctobacterium、Aminobacterium、Saccharofermentans与Alkaliflexus(所占比例之和为24.93%);产氢产乙酸菌群Petrimonas与Syntrophomonas(所占比例之和为34.89%);产甲烷菌Methanosaeta(3.44%)及可耐受工业废水毒害的微生物菌群Levilinea、Longilinea与Thermovirga(所占比例之和为14.62%).
[Abstract]:In order to reveal the mechanism of functional microflora in the biological treatment of citric acid wastewater, the anaerobic granular sludge in the inner circulating anaerobic reactor of citric acid industrial wastewater was used as the research object, and the particle size distribution was calculated. The microscopic morphology of particles was observed by environmental scanning electron microscope (ESEM), and the microbial diversity and microflora characteristics were analyzed by high-throughput sequencing technique. The results showed that the particle size of 1.0 ~ 4.0 mm was the largest. ESEM showed that the microbes were mainly globular bacteria, and 8397 effective sequences were obtained by high-throughput sequencing. The ACE index and Chao1 index were 3 415.51 and 2 246.51, respectively, which indicated that the diversity index of 873 OTU units showed that the sample library coverage was 0.936 and the ACE index and Chao1 index were 3 415.51 and 2 246.51, respectively, which indicated that there were more microbes and microbes in granular sludge. The group consists of four main categories, Paludibacter paludibacterium Parabacteroidesus Erysipelotrichaceaeae Clostridium phascolarctoibacterium Saccharofermentans and Alkaliflexus (the sum is 24.93333N); hydroacetic acidogenic Petrimonas and trophSynomonas (34.89%; methanosaeta3.44); and Levilina Longilinea and Thervirgaea (the proportion is 14.62g).
【作者单位】：南华大学矿业工程博士后流动站;污染控制与资源化技术湖南省高校重点实验室(南华大学);铀矿冶生物技术国防重点学科实验室(南华大学);
【基金】：国家自然科学基金(51408293) 中国博士后基金面上项目(2014M562114) 湖南省教育厅优秀青年项目(14B154)
【分类号】：X703;X172

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