产甲烷菌耐酸培养及其以餐厨为底物的产气特性

发布时间：2018-03-18 23:09

本文选题：餐厨垃圾　切入点：高固态发酵　出处：《环境工程学报》2016年11期 　论文类型：期刊论文

【摘要】：采用逐步提高厌氧发酵体系内丁酸盐浓度的方式,对产甲烷菌进行了适应性耐酸培养,随后将培养后的污泥接种餐厨垃圾进行高固态厌氧发酵,研究其以餐厨垃圾为底物的产甲烷特性。结果表明,进行耐丁酸培养的各个组的沼气及甲烷产量、最终VFAs浓度、SCOD浓度基本和丁酸钠添加量呈正相关。其中丁酸钠添加量为8 g·L-1的组的甲烷产量和沼气产量相对稳定且较大,最终分别为229 m L和830 m L。其脱氢酶活性也相对较大,为126.74 TFμg·(m L·h)-1;培养后污泥的TS、VS较培养前提高量最大,分别提高了64.13%和67.31%,相比培养后的对照组也提高了44.05%和66.07%。而产甲烷实验研究表明,经8 g·L-1的丁酸钠培养后的污泥的产气情况最好,其累积甲烷、沼气产率及甲烷含量最大,分别为101.8 m L·g~(-1)TS、228.0 m L·g~(-1)TS和44.7%,是对照组的3.2、1.3和2.4倍。
[Abstract]:The methanogenic bacteria were cultured with adaptive acid tolerance by increasing the concentration of butyrate in anaerobic fermentation system, and then the sludge was inoculated into kitchen waste for high solid anaerobic fermentation. The methanogenic properties of different groups of butyric acid resistant waste were studied, and the results showed that the methane production and methane production of each group were determined by using butyric acid as the substrate. The final VFAs concentration and Scod concentration were positively correlated with sodium butyrate, and the methane and biogas production of the group with 8 g 路L -1 sodium butyrate was relatively stable, and the final values were 229 mL and 830 mL, respectively. The dehydrogenase activity of the group with 8 g 路L -1 sodium butyrate was relatively high. It was 126.74 TF 渭 g 路m L 路hn-1, and the amount of TSV S in cultured sludge was increased by 64.13% and 67.31, respectively, and 44.05% and 66.07 by comparison with the control group. The results of methanogenic experiment showed that the sludge cultured with 8 g 路L ~ (-1) sodium butyrate had the best gas production, and the results were as follows: (1) compared with the control group, the gas production of the sludge cultured with 8 g 路L ~ (-1) of sodium butyrate was the best. The accumulative methane, biogas yield and methane content were 101.8 mL 路g ~ (-1), 228.0 mL 路g ~ (-1) T _ (-1) and 44.7%, respectively, which were 3.21.3 and 2.4 times of those of the control group.
【作者单位】：江南大学环境与土木工程学院;江苏省厌氧生物技术重点实验室;
【基金】：江苏省科技支撑计划项目(BE2012615) 江苏省自然科学基金项目(BK20130126)
【分类号】：X799

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