甲醛暴露时间延长加剧小鼠哮喘模型肺氧化损伤并促进IL-17表达

发布时间：2018-03-19 03:18

本文选题：甲醛　切入点：小鼠　出处：《生态毒理学报》2017年01期 　论文类型：期刊论文

【摘要】：为了探究不同暴露时间甲醛对小鼠哮喘模型肺氧化应激及IL-17表达的影响,用浓度为3.0 mg·m~(-3)的甲醛气体吸入染毒,同时将48只雄性Balb/c小鼠随机分为6组:(1)对照组(生理盐水组);(2)ovalbumin(OVA)致敏组;(3)0.5 h甲醛+OVA组;(4)1h甲醛+OVA组;(5)1.5 h甲醛+OVA组;(6)2 h甲醛+OVA组,以不同时间长度进行甲醛暴露,连续35 d。OVA致敏组、0.5 h甲醛+OVA组、1 h甲醛+OVA组、1.5 h甲醛+OVA组、2 h甲醛+OVA组均在第11、18及25天腹腔注射OVA致敏液(5 mg OVA+175 mg Al(OH)_3+30 mL生理盐水),第29~35天(共计1周)进行1%OVA雾化(30 min·d~(-1)),每日1次,诱发哮喘。第36天进行以下操作:取肺组织测定肺系数并制作肺匀浆,检测肺组织中活性氧自由基(ROS)、丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量,并采用ELISA法检测肺组织中IL-17的水平。同时,采用HE染色法观察小鼠肺部气道的病理学变化。结果显示,在浓度为3.0 mg·m~(-3)的甲醛气体吸入染毒条件下,与对照组相比,1.5 h甲醛+OVA染毒组、2 h甲醛+OVA染毒组ROS、MDA、IL-17含量上升,具有统计学意义(P0.01)。同时,随着暴露时间长度的增加,小鼠肺部气道出现明显病理学变化。综上所述,每天2 h甲醛+OVA染毒能对小鼠肺造成损伤并恶化OVA对小鼠肺的损伤,产生炎症反应,并通过氧化应激反应介导。
[Abstract]:In order to investigate the effects of formaldehyde exposure at different exposure time on lung oxidative stress and IL-17 expression in mice with asthma, the mice were exposed to formaldehyde gas at concentration of 3. 0 mg 路m ~ (-1) -3). At the same time, 48 male Balb/c mice were randomly divided into 6 groups: control group (normal saline group) and control group (normal saline group) sensitized with formaldehyde for 30.5 h, OVA group for 4h, OVA group for 51.5 h, formaldehyde group for 6 h and OVA group for 2 h. Formaldehyde exposure was carried out at different length of time. Continuous 35 d.OVA sensitized group: 0. 5 h formaldehyde OVA group 1 h formaldehyde OVA group 1. 5 h formaldehyde OVA group 2 h formaldehyde OVA group were intraperitoneally injected 5 mg OVA 175 mg Al(OH)_3 30 mL normal saline at 1118 and 25 days, 2935 days (a total of 1. ) take 1 OVA atomization of 30 min 路dac-1, once a day, To induce asthma. On the 36th day, the lung tissue was taken to measure the lung coefficient and to make the lung homogenate. The contents of reactive oxygen species (Ros), malondialdehyde (MDA) and glutathione (GSH) in the lung tissue were measured. The level of IL-17 in lung tissue was detected by ELISA method, and the pathological changes of lung airway in mice were observed by HE staining. Compared with the control group, the content of IL-17 in rosiform MDA-MDA-IL-17 was increased at 2 h after exposure to formaldehyde OVA, and there was a significant difference between the two groups (P 0.01). At the same time, with the increase of exposure time, there were obvious pathological changes in the lungs of mice. Formaldehyde OVA exposure for 2 hours per day could cause lung injury in mice and worsen the lung injury induced by OVA, resulting in inflammatory response mediated by oxidative stress.
【作者单位】：华中师范大学生命科学学院;重庆大学城市建设与环境工程学院;
【基金】：国家自然科学基金面上项目(No:21577045)
【分类号】：X171.5

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