鸡、猪粪便源大肠杆菌对4种抗生素耐药性的比较

发布时间：2018-03-25 17:46

  本文选题：抗生素　切入点：大肠杆菌　出处：《生态毒理学报》2017年01期

【摘要】：以琼脂稀释法对67株鸡源大肠杆菌及61株猪源大肠杆菌进行最低抑菌浓度(MIC)测定,并以SPSS分别进行卡方检验和Probit模型估算,分析不同来源大肠杆菌耐药差异显著性及不同抗生素的半数抑菌浓度(MIC50),以期对不同畜禽粪便来源的大肠杆菌耐药差异进行详细准确的探讨。结果显示,鸡源大肠杆菌对头孢噻肟、诺氟沙星、环丙沙星和四环素耐药率分别为98.51%、68.66%、56.72%和100%,猪源大肠杆菌对头孢噻肟耐药率为88.52%,对诺氟沙星、环丙沙星和四环素则100%耐药。除四环素,鸡源、猪源大肠杆菌对诺氟沙星、环丙沙星、头孢噻肟的耐受差异显著(P0.05),51%的鸡源大肠杆菌和89%的猪源大肠杆菌均呈4重耐药。SPSS分析结果表明,Probit模型估算结果优于当前MIC50常规计算方法,头孢噻肟、诺氟沙星、环丙沙星和四环素对鸡源大肠杆菌MIC50分别为40.031μg·m L~(-1)、40.020μg·m L~(-1)、2.683μg·m L~(-1)和101.418μg·m L~(-1),对猪源大肠杆菌MIC50分别为8.724μg·m L~(-1)、56.044μg·m L~(-1)、31.214μg·m L~(-1)和130.915μg·m L~(-1)。回归方程显示环丙沙星对鸡源大肠杆菌抑制作用最强;高于40.031μg·m L~(-1)时,诺氟沙星抗菌作用弱于头孢噻肟,低于120.23μg·m L~(-1)时,诺氟沙星抗菌作用强于四环素;头孢噻肟、环丙沙星、诺氟沙星和四环素对猪源大肠杆菌的抑菌作用则呈递减趋势。同时验证了诺氟沙星和环丙沙星属同种作用机制,基于Probit模型计算更简单、快速、直观。研究结果可为畜禽养殖中大肠杆菌的耐药差异监控提供一定的数据基础。
[Abstract]:Minimum inhibitory concentration of 67 Escherichia coli and 61 strains of Escherichia coli by agar dilution method (MIC) and SPSS were determined by chi square test and Probit model to estimate and analysis of half inhibitory concentration of different sources of drug resistance of Escherichia coli and significantly different antibiotics (MIC50), for different livestock manure the source of the drug resistance of Escherichia coli in order to explore the differences of detail and accurate. The results showed that Escherichia coli of norfloxacin, cefotaxime, ciprofloxacin and tetracycline resistance rates were 98.51%, 68.66%, 56.72% and 100%, Escherichia coli cefetaxime resistant rate was 88.52%, of norfloxacin, ciprofloxacin and tetracycline were 100% resistance. Except for tetracycline, ciprofloxacin in chicken, Escherichia coli, tolerance to norfloxacin, cefotaxime was significantly difference (P0.05), Escherichia coli 51% Escherichia coli and 89% were 4閲嶈，

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