氟喹诺酮抗生素对厌氧氨氧化菌活性抑制研究

发布时间：2018-04-16 11:27

本文选题：厌氧氨氧化 + 诺氟沙星　；参考：《大连理工大学》2015年硕士论文

【摘要】：厌氧氨氧化(Anaerobic ammonium oxidation)技术是一种新型的生物脱氮技术,它的反应原理是在厌氧或缺氧条件下,厌氧氨氧化微生物以亚硝酸氮为电子受体,以氨氮为电子供体,将两者转化为氮气。该技术具有不会产生温室气体、无需曝气和无需添加碳源等优点。利用厌氧氨氧化技术低耗处理实际废水已成为近年来环境领域的一大研究热点。但是,由于抗生素的大量使用,除制药废水、医疗废水和蓄禽养殖废水外,甚至是生活污水中均检测到抗生素的存在。由于各类污水中抗生素的存在不可避免,因此,有必要进行抗生素对厌氧氨氧化菌的活性抑制研究。本课题的主要研究方向是考察实际废水中含量较高的氟喹诺酮类抗生素诺氟沙星和左氧氟沙星对厌氧氨氧化菌活性的抑制作用。通过短期实验和长期的抑制实验,考察了上述两种抗生素对厌氧氨氧化菌活性的抑制效果。主要研究结果如下：(1)在短期实验中,当诺氟沙星的添加浓度达200 mg/L时,总氮脱除速率从0.546 mgN/g VSS/h降至0.315 mg N/g VSS/h,是空白对照组的57.6%。当左氧氟沙星添加浓度为400mg/L时,总氮脱除效率从0.762 mg N/g VSS/h降至0.4 mg N/g VSS/h,是空白对照组的52.5%。(2)诺氟沙星的半抑制浓度(ICs0)为227.39 mg/L,左氧氟沙星的IC50为386.75 mg/L,诺氟沙星对厌氧氨氧化污泥的抑制作用强于左氧氟沙星。(3)在长期实验中,当进水中诺氟沙星的浓度达10 μg/L时,不会对厌氧氨氧化污泥产生较为明显的抑制效果,脱氮百分比和总氮脱除率均值分别为76.75%和0.586kgN/m3/d,与对照组的78.59%和0.599 kg N/m3/d相比较为接近。(4)在长期实验中,当进水诺氟沙星的浓度从25增至50 mg/L时,不会对厌氧氨氧化污泥的活性产生明显的抑制效果,当诺氟沙星浓度达75 mg/L时,厌氧氨氧化污泥的活性受到明显抑制,脱氮百分比和总氮脱除率分别下降至50.51%和0.445 kg N/m3/d。(5)在长期实验中,当进水浓度始终保持150 mg/L时,会对厌氧氨氧化污泥的活性产生明显的抑制效果,反应器运行36 d后完全崩溃,脱氮百分比和总氮脱除率分别下降至8.34%和0.073 kg N/m3/d。
[Abstract]:Anaerobic ammonia oxidation (anaerobic ammonium oxidation) technique is a new biological denitrification technique. Its reaction principle is that the anaerobic ammonia-oxidizing microorganism takes nitrite nitrogen as electron receptor and ammonia nitrogen as electron donor to convert both into nitrogen under anaerobic or anoxic conditions.The technology has the advantages of no greenhouse gas, no aeration and no carbon source.Using anaerobic ammonia oxidation technology to treat practical wastewater with low consumption has become a research hotspot in the field of environment in recent years.However, due to the extensive use of antibiotics, antibiotics are detected in the domestic sewage, except pharmaceutical wastewater, medical wastewater and poultry storage wastewater.Because of the inevitable existence of antibiotics in various kinds of sewage, it is necessary to study the activity inhibition of antibiotics to anaerobic ammonia oxidizing bacteria.The main research direction of this paper is to investigate the inhibitory effect of norfloxacin and levofloxacin on the activity of anaerobic ammonia oxidizing bacteria with high content of fluoroquinolones in actual wastewater.The inhibitory effects of these two antibiotics on the activity of anaerobic ammonia-oxidizing bacteria were investigated by short-term and long-term inhibition experiments.The main results were as follows: (1) in the short-term experiment, when the concentration of norfloxacin reached 200 mg/L, the removal rate of total nitrogen decreased from 0.546 mgN/g VSS/h to 0.315 mg N / g VSS / h, which was 57.6% of the control group.When levofloxacin was added with a concentration of 400mg/L,The total nitrogen removal efficiency decreased from 0.762 mg N / g VSS/h to 0.4 mg N / g VSS / h, which was 52.5% of the blank control group. ICs0) of norfloxacin was 227.39 mg / L, the IC50 of levofloxacin was 386.75 mg / L, and the inhibitory effect of norfloxacin on anaerobic ammonia oxidation sludge was stronger than that of levoxacin on anaerobic ammonia oxidation sludge.In long-term experiments,When the concentration of norfloxacin in the influent reaches 10 渭 g / L, the anaerobic ammonia oxidation sludge will not be inhibited significantly.The average denitrification percentage and total nitrogen removal rate were 76.75% and 0.586kgN / m ~ (3 / d) respectively, which were similar to those of the control group (78.59% and 0.599 kg N/m3/d, respectively.) in the long-term experiment, when the concentration of norfloxacin in the influent increased from 25 to 50 mg/L,When the concentration of norfloxacin reached 75 mg/L, the activity of anaerobic ammonia oxidized sludge was significantly inhibited.The percentage of nitrogen removal and the removal rate of total nitrogen decreased to 50.51% and 0.445 kg N / m ~ 3 / d 路m ~ (5) respectively. In a long-term experiment, when the influent concentration was kept at 150 mg/L, the activity of anaerobic ammonia oxidized sludge was inhibited obviously, and the reactor completely collapsed after 36 days of operation.The percentage of nitrogen removal and the removal rate of total nitrogen decreased to 8.34% and 0.073 kg N / m 3 / d, respectively.
【学位授予单位】：大连理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X703

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