固定化漆酶磁性纳米材料的合成及对合成染料的降解

发布时间：2018-04-16 12:04

本文选题：固定化漆酶 + 磁性纳米材料　；参考：《环境工程》2017年03期

【摘要】：介绍了一种温和无损固定化漆酶的方法,并研究了其对合成染料的降解行为。首先采用共沉淀法合成乙二胺四乙酸(EDTA)功能化的磁性纳米粒子,并以Cu2+为桥基,通过螯合作用将漆酶固载于EDTA功能化的磁性纳米粒子表面。通过透射电镜、紫外-可见光谱仪等对磁性固定化漆酶纳米材料进行了表征。固定化漆酶磁性纳米材料尺寸在15 nm左右,具有良好的磁响应能力,漆酶固定化率可达223 mg/g;在最佳降解条件下,其对孔雀石绿和碱性品红均表现出良好的降解能力;同时固定化漆酶具有较高的稳定性和重复使用性,便于回收利用。
[Abstract]:A mild and nondestructive immobilization method of laccase was introduced, and the degradation behavior of synthetic dyes was studied.Firstly, the ethylenediamine tetraacetic acid (EDTA) functionalized magnetic nanoparticles were synthesized by co-precipitation method, and the laccase was immobilized on the surface of EDTA functionalized magnetic nanoparticles by chelating with Cu2 as the bridge.Magnetic immobilized laccase nanomaterials were characterized by transmission electron microscopy (TEM) and UV-vis spectrometer.The size of immobilized laccase magnetic nanomaterials is about 15 nm and has good magnetic response. The immobilization rate of laccase can reach 223 mg / g. Under the optimum degradation conditions, the immobilized laccase shows good degradation ability to both malachite green and alkaline fuchsin.At the same time, the immobilized laccase has high stability and reusability, which is easy to recycle.
【作者单位】：新疆产品质量监督检验研究院;新疆恒正司法鉴定中心;
【基金】：国家自然科学基金项目(21177013)
【分类号】：X703;TB383.1

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