耐受重金属铅乳酸菌的分离筛选及鉴定

发布时间：2018-04-23 15:01

  本文选题：尾矿区 + 乳酸菌　； 参考：《食品工业科技》2016年22期

【摘要】：通过对包头尾矿库区、白云鄂博采矿区重金属污染严重区域土壤样品中的乳酸菌进行分离、筛选,共得到62株乳酸菌,并对分离得到的乳酸菌进行耐受铅离子的测定,62株乳酸菌对铅离子均有一定的耐受能力,不同菌株对铅离子的耐受能力有所不同;铅离子浓度为1200~9600 mg/L时,随着培养时间的增长,菌株对耐受铅离子的修复能力逐渐增强;30℃下分离得到的菌株对铅离子的修复能力明显优于37℃下分离得到的菌株;菌株BY-Fa4、BG-5b3、BG-8b2等13株菌可在铅离子浓度为7200 mg/L的环境下生长,菌株BY-Fa1、BY-Fc2、BG-6b1可在铅离子浓度为8400 mg/L的环境下生长。实验结果得出筛选得到的13株乳酸菌对铅离子不仅具有较强的耐受能力,而且还具有较强的修复能力。对筛选出13株高耐受铅离子的菌株进行16S rRNA序列分析,鉴定结果为戊糖片球菌2株、清酒乳杆菌1株、食窦魏斯氏菌5株、戊糖乳杆菌2株、植物乳杆菌3株。
[Abstract]:A total of 62 strains of lactic acid bacteria were isolated from soil samples in Baotou tailing area and heavy metal polluted area of Baiyunebo mining area, and 62 strains of lactic acid bacteria were obtained. The results showed that 62 strains of lactic acid bacteria had certain tolerance to lead ion, and different strains had different tolerance ability to lead ion, and when lead ion concentration was 1 200 ~ 9 600 mg/L, the tolerance ability of different strains to lead ion increased with the increase of culture time. The ability of repairing lead ion tolerance of the strain was gradually enhanced, and the repair ability of the strain isolated at 30 鈩，

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