滇池铜绿微囊藻生长启动、最大增长的春化过程等研究

发布时间：2018-05-08 07:17

  本文选题：滇池铜绿微囊藻 + 春化过程　； 参考：《重庆大学》2016年硕士论文

【摘要】：2009-2014年实验证实滇池铜绿微囊藻具有春化作用,这是一项重要的科学发现,人类开始认识到铜绿微囊藻的生长活动对低温有需求。为认识滇池铜绿微囊藻大暴发的条件,需要了解该藻高温休眠后藻生长启动的低温诱导关系、藻大暴发需要的春化过程,以及冬季变化的低温与藻春化过程的关系等,为此,开展了不同变温实验和诱导低温连续诱导实验,分析藻春化过程与诱导低温间的关系,研究藻高温休眠后藻生长启动的春化过程和藻最大增长率的春化过程、低温间的掩盖作用、间断春化过程的累积作用等。实验结果与结论如下:结果与分析:(1)藻生长启动的春化过程(诱导时间/诱导低温)实验中,31℃高温休眠处理1d后,最短诱导时间与诱导温度的关系呈“V”型,藻生长启动的春化过程为12h/14℃、11h/13℃、9h/12℃、7h/11℃、6h/10℃、7h/9℃、8h/8℃、10h/7℃,10℃为最适诱导低温。(2)藻增长的最大春化过程实验中,藻增长率随着诱导时间的延长而增加,增长率与诱导时间呈正相关。当处理时间达到45d时,藻增长率由最初的17.7%达到最大值42.2%。表明10℃低温作用于藻时,藻最大增长率的春化过程是45d/10℃。(3)诱导低温间的掩盖作用实验中,当处理温度由14℃→13℃→12℃→11℃→10℃降低时,增长率从1.1%上升到27.0%;,当处理温度由10℃→9℃→8℃→7℃降低时,增长率从27.0%下降到3.8%,诱导低温间有掩盖作用。(4)诱导低温间的累积作用实验中,当处理温度由14℃→13℃→12℃→11℃→10℃降低时,10℃间断出现共12d,12d时的增长率为27.0%,与10℃连续处理处理12d的增长率26.2%一致,说明诱导低温间具有累积作用;当处理温度由10℃→9℃→8℃→7℃降低时,诱导低温间不具有累积作用。(5)滇池铜绿微囊藻生长的饱和密度实验中,藻类经低温诱导后的生长趋势都为:藻增长缓慢→藻增长快速→藻增长趋于平稳→藻逐渐死亡,当藻增长趋于平衡时藻达到饱和密度为1010个/L。(6)对滇池铜绿微囊藻的定义讨论分析,滇池的铜绿微囊藻的最适生长温度为19℃,太湖的铜绿微囊藻为25℃。研究结论:(1)最适诱导低温的藻生长启动的春化过程最短。(2)滇池铜绿微囊藻暴发需要特定的春化过程(低温过程)。(3)强诱导低温掩盖弱诱导低温的藻春化作用,但在10~7℃内,这种掩盖作用是短时间的。(4)诱导低温在14~10℃波动对藻作用过程中,最强低温的间断春化过程具有累积作用,而诱导低温在10~7℃波动交替对藻作用过程中,最强低温的间断春化过程则没有累积作用。(5)滇池的铜绿微囊藻藻生长的饱和密度为1010个/L。(6)生长在滇池的铜绿微囊藻是铜绿微囊藻中适应云贵高原湖泊的一个生态型,即滇池铜绿微囊藻,学名Microcystis aeruginosa in Dianchi Lake。
[Abstract]:The results of 2009-2014 experiments proved that microcystis aeruginosa has vernalization in Dianchi Lake, which is an important scientific discovery. Human beings are beginning to realize that the growth of microcystis aeruginosa needs low temperature. In order to understand the conditions of microcystis aeruginosa outbreak in Dianchi Lake, it is necessary to understand the low temperature induction relationship of algae growth initiation after high temperature dormancy, the vernalization process required by algal outbreak, and the relationship between the low temperature variation in winter and the vernalization process of algae. Different experiments of changing temperature and continuous induction of low temperature were carried out to analyze the relationship between the process of algal vernalization and induced low temperature, and to study the process of vernalization of algae growth and the process of maximum growth rate of algae after high temperature dormancy. The concealment between low temperature and the accumulation of intermittent vernalization. The experimental results and conclusions are as follows: results and analysis of the vernalization process (induction time / induced low temperature) initiated by the growth of the algae, the relationship between the shortest induction time and the induced temperature was "V" type after dormancy treatment at 31 鈩，

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