贫营养好氧反硝化菌群GNY净化微污染水源水试验研究

发布时间：2018-05-11 21:41

本文选题：贫营养 + 好氧反硝化细菌　；参考：《西安建筑科技大学》2015年硕士论文

【摘要】：本研究针对微污染水源水的氮源污染问题,深入研究了贫营养好氧反硝化细菌的脱氮特性,在课题组前期分离的23株贫营养好氧反硝化细菌中筛选并组合得到了高效好氧反硝化功能菌群,讨论了优势生态菌群的最佳投菌量和对微污染水源水的脱氮效能的影响。主要研究结果包括以下几个方面:(1)从水库沉积物中筛选到一株好氧反硝化细菌Acinetobacter sp.Sxf14,对该菌株脱氮特性进行研究,并将其接种到C/N(总有机碳与总氮的比值)为1.2的微污染水库源水中,以探究其对实际源水总氮的去除效果.结果显示:Sxf14能以硝酸盐和亚硝酸盐为唯一氮源进行好氧反硝化.反应48 h后,NO3--N和NO2--N的去除率分别达74.84±0.86%和40.52±1.49%,TN去除率最高达到65.07±1.56%和41.33±0.98%;72 h内,微污染水库源水的TN浓度由2.46±0.02 mg/L降到1.68±0.01 mg/L,去除率达到31.7±0.14%。因此,该菌株具有反硝化能力,能承受较低的碳氮比,降低微污染源水中的氮素,本研究可为微污染水体的菌剂修复技术提供科学依据。(2)采用菌源组合构建出优势贫营养好氧反硝化功能菌群GNY(G107+N299+81Y),研究不同碳氮比对组合菌群脱氮性能的影响,结果表明:随着碳氮比值的增大,好氧反硝化菌的反应速率逐渐增强,功能菌群能够适应贫营养环境,当C/N为3,硝氮去除率达到57.18%,总氮去除率达到43.52%,且能维持较长时间的好氧反硝化活性。(3)将生态菌群GNY分别接入水库纯源水和灭菌后的水库源水小试中,NO3--N最高去除率达到35.9%,TN最高去除率达到46.78%;在纯源水培养基中,NO3--N最高去除率达到27.97%,TN最高去除率达到43.58%,投菌组比对照组的硝氮去除率高15.26%,,总氮去除率高17.38%;说明该生物菌群可以适应源水贫营养环境,并能够有效地降低微污染水体中的氮素。(4)在水库中层7.5m水深处进行投菌量梯度挂瓶实验,结果显示0.1ppm的投菌量梯度脱氮效果最好,NO3--N去除率最高达到66.49%,TN去除率最高达到53.52%;在水库源水静态投菌量梯度试验中,0.1ppm投菌量梯度脱氮效果最好,NO3--N和TN去除率最高分别达到41.44%和45.16%,可以得出该生态菌剂的最佳投菌量梯度为0.1ppm。(5)在原水TOC为6.40mg/L,TN浓度为1.85mg/L条件下,按照0.1ppm菌剂投加量,对贫营养好氧反硝化生态菌剂进行中试试验研究,结果表明,投菌系统的脱氮效果明显好于对照系统,投菌组在系统运行稳定后,硝氮去除率维持在43.57%左右,比对照系统高22.34%,总氮去除率维持在51.32%左右,比对照系统高19.34%;与此同时,投菌组生物量明显高于对照组,好氧反硝化细菌总数也明显高于对照组;表明该菌剂可用于微污染水源水处理工程应用。
[Abstract]:In order to solve the problem of nitrogen source pollution in micro-polluted source water, the denitrification characteristics of aerobic denitrifying bacteria with poor nutrition were studied. 23 strains of aerobic denitrifying bacteria isolated from the early stage of the research group were screened and combined to obtain the high efficient aerobic denitrification functional flora. The optimal amount of bacteria in the dominant ecological flora and the effect on the denitrification efficiency of the micro-polluted source water were discussed. The main results were as follows: (1) A aerobic denitrifying bacterium Acinetobacter sp. Sxf14 was screened from reservoir sediment, and the denitrification characteristics of this strain were studied. It was inoculated into the source water of micro-polluted reservoir with C / N ratio of 1.2 to explore the removal effect of total nitrogen in real source water. The results showed that: Sxf14 could take nitrate and nitrite as sole nitrogen sources for aerobic denitrification. After 48 h reaction, the removal rates of NO3-N and NO2--N were 74.84 卤0.86% and 40.52 卤1.49mg / L, respectively. The highest removal rates of TN were 65.07 卤1.56% and 41.33 卤0.9872 h, respectively. The TN concentration of the source water of the micro-polluted reservoir decreased from 2.46 卤0.02 mg/L to 1.68 卤0.01mg / L, and the removal rate reached 31.7 卤0.14g / L. Therefore, the strain has the ability to denitrification, can withstand lower carbon to nitrogen ratio, reduce nitrogen in micro-pollution water, This study can provide scientific basis for microorganism remediation technology of micro-polluted water body. (2) A dominant nutrient deficient aerobic denitrification functional flora GNY(G107 N29981Y was constructed by using microbial source combination, and the effect of different carbon / nitrogen ratio on denitrification performance of the combination was studied. The results showed that the reaction rate of aerobic denitrifying bacteria increased with the increase of C / N ratio, and the functional bacteria could adapt to the poor nutrition environment. When C / N was 3, nitrate removal rate was 57.18, total nitrogen removal rate was 43.52, and aerobic denitrification activity could be maintained for a long time.) Eco-microflora GNY was connected to reservoir pure source water and sterilized reservoir source water respectively to get the highest removal rate of NO3 N. The highest removal rate of TN was 46.78 in pure water medium, the highest removal rate of nitrogen was 27.97% in pure water medium, the removal rate of nitrate nitrogen and total nitrogen was 15.26% and 17.38% higher than that of the control group, which indicated that the bacteria group could adapt to the environment of source water and nutrition, and the total nitrogen removal rate was 17.38% higher than that of the control group, and the highest removal rate of nitrogen was 27.97% in the pure water medium, and the removal rate of nitrate nitrogen was 15.26% and 17.38% higher than that in the control group. And it can effectively reduce the nitrogen content in the slightly polluted water, and carry out the bottling experiment in the depth of 7.5 m water depth of the middle layer of the reservoir. The results showed that 0.1ppm had the best removal rate of no _ 3 N and the highest removal rate of TN was 66.49% and 53.52% respectively, and the highest removal rate of no _ 3 N and TN in static gradient test of reservoir source water was 0.1 ppm. The optimum dosage gradient of the ecological agent is 0.1ppm.mg. 5) when the TOC of raw water is 6.40 mg / L ~ (TN), the concentration of TN is 6.40 mg / L ~ (-1) N ~ (2 +), and the concentration of TN is 6.40 mg 路L ~ (-1) 路L ~ (-1) 路m ~ (-1) ~ (-1). According to the dosage of 0.1ppm, a pilot experiment was carried out on the aerobic and denitrification ecological agent for poor nutrition. The results showed that the denitrification effect of the system was obviously better than that of the control system. The removal rate of nitrate nitrogen was about 43.57%, which was 22.34% higher than that of the control system, and the removal rate of total nitrogen was about 51.32%, which was 19.34% higher than that of the control system, at the same time, the biomass and the total number of aerobic denitrifying bacteria in the inoculated bacteria group were obviously higher than those in the control group. The results show that the microorganism can be used in water treatment engineering of micro-polluted source water.
【学位授予单位】：西安建筑科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X52;X172

【相似文献】