SRT对富集高聚PHA能力嗜盐MMC的影响

发布时间：2018-05-12 01:35

本文选题：SRT + PHA积累菌　；参考：《化工学报》2016年06期

【摘要】：调控菌群富集过程的工艺参数,富集具有高PHA积累能力的混合菌(MMC)是发酵生产PHA的第一步也是最重要的一步。采用批式培养条件,集中研究了污泥停留时间(SRT)对PHA积累菌群的富集作用。研究结果表明SRT影响富集MMC的底物降解速率(qS),微生物积累PHA的速率(qP)和PHA积累能力。长SRT(SRT=4 d)导致了较低的qS,qP和PHA细胞含量;过短的SRT(SRT=1 d)则降低了MMC的PHA转化率(YPHA/S)和PHA积累能力,刺激了非PHA积累菌群的快速增殖。研究确定的最佳SRT为2 d,在此条件下富集的MMC最大PHA积累量可达细胞干重的56%。研究证明了SRT在选择嗜盐MMC中的重要作用,为快速富集PHA积累能力MMC奠定基础。
[Abstract]:It is the first and most important step of fermentation to produce PHA by regulating the technological parameters of the enrichment process and enriching the mixed bacteria with high accumulation ability of PHA. The enrichment of PHA accumulation by sludge retention time (SRT) was studied by batch culture. The results showed that SRT affected the substrate degradation rate of MMC enrichment, the accumulation rate of PHA by microorganism and the accumulation ability of PHA. Long SRT(SRT=4 d) resulted in lower cell content of qSN QP and PHA, while too short SRT(SRT=1 d) decreased the PHA conversion rate of MMC, YPHA / S) and PHA accumulation ability, and stimulated the rapid proliferation of non PHA accumulative bacteria. The optimum SRT was determined to be 2 days. Under this condition, the maximum accumulation of MMC PHA could reach 56% of the dry weight of cells. The results show that SRT plays an important role in the selection of halophilic MMC and lays the foundation for rapid accumulation of PHA accumulation ability MMC.
【作者单位】：北京工业大学环境与能源工程学院;
【基金】：国家自然科学基金项目(51478011,51178004) 北京市自然科学基金项目(8132013) 北京市属高等学校高层次人才引进与培养计划项目~~
【分类号】：X703;X172

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