耐受重金属铅的乳酸菌分离筛选及鉴定

发布时间：2018-06-01 08:42

本文选题：尾矿区 + 乳酸菌　；参考：《内蒙古农业大学》2016年硕士论文

【摘要】：内蒙古地区矿产资源丰富,在矿产资源开采和利用的过程中会对环境造成一定的污染,据资料显示包头尾矿库区、白云鄂博采矿区重金属污染指数比背景值高出9倍,人们的健康安全受到了极大的威胁。虽然生物吸附去除重金属的研究比较热门,但对实际应用到人体内作为重金属生物吸附剂的微生物研究还处于刚起步阶段。本研究通过采集包头尾矿库区、白云鄂博采矿区重金属污染严重区域的土壤样品,对土壤样品中的乳酸菌进行分离、筛选,共得到62株乳酸菌,将分离得到的乳酸菌接种于含不同铅离子浓度的培养基中进行培养,观察乳酸菌对铅离子的耐受情况,试验得出分离得到的62株乳酸菌对铅离子均有一定的耐受能力,不同菌株对铅离子的耐受能力有所不同；随着培养时间的增长,菌株对耐受铅离子的修复能力逐渐增强；30℃下分离得到的菌株对铅离子的修复能力明显优于37℃下分离得到的菌株；铅离子浓度为1200-9600mg/L时,随着培养时间的增长,分离菌株表现出对铅离子有较强的修复能力。菌株BY-Fa4、BG-5b3、BG-8b2等13株菌可在铅离子浓度为7200mg/L的环境下生长,菌株BY-Fa1、BY-Fc2、BG-6b1可在铅离子浓度为8400mg/L的环境下生长,以上菌株均表现出较强的耐受铅的能力。对筛选出13株高耐受铅离子的菌株进行形态学、生理生化鉴定和16S rRNA序列分析,结果得出,菌株BG-10a1、BG-5b3为戊糖片球菌(Pediococcus pentosaceus),菌株BG-6bl为清酒乳杆菌(Lactobacillus sakei),菌株BY-Fa5、BY-Ff3、BY-Fe21、BY-Fe3、BG-8b2为食窦魏斯氏菌(Weissella cibaria),菌株BY-Fa2、BY-Fa4为戊糖乳杆菌(Lactobacillus pentosus),菌株BY-Fa1、BY-Fc2、BY-Ff4为植物乳杆菌(Lactobacillus plantarum)。
[Abstract]:Inner Mongolia area is rich in mineral resources. It will cause some pollution to the environment during the mining and utilization of mineral resources. According to the data, the heavy metal pollution index in the Baiyunebo mining area is 9 times higher than that of the background value, and the health and safety of people are greatly threatened. Although the biological adsorption of heavy metals is studied by biological adsorption However, the study of microorganism used as a heavy metal biosorption agent in the human body is still in the initial stage. By collecting the soil samples from the Baotou tailings reservoir area and the heavy metal pollution area of the Baiyunebo mining area, the lactic acid bacteria in the soil samples were separated and screened, and 62 strains of lactic acid bacteria were obtained. The isolated lactic acid bacteria were inoculated in culture medium containing different lead ions concentration, and the tolerance of lactic acid bacteria to lead ions was observed. The experimental results showed that 62 strains of lactic acid bacteria were tolerated to lead ions, and the tolerance of different strains to lead ions was different. The ability to repair lead ions was enhanced gradually, and the ability to repair lead ions at 30 centigrade was better than that of the strain at 37. When the concentration of lead ion was 1200-9600mg/L, the isolation strain showed a stronger ability to repair lead ions with the increase of culture time. The strain BY-Fa4, BG-5b3, BG-8b2 and so on were 1. The 3 strains can grow in the environment of lead ion concentration of 7200mg/L. Strain BY-Fa1, BY-Fc2, BG-6b1 can grow under the environment of 8400mg/L. All the above strains show strong ability to tolerate lead. 13 strains of high tolerance lead ions were selected for morphology, physiological and biochemical identification and 16S rRNA sequence analysis. The results were obtained. Strain BG-10a1, BG-5b3 is pentose Streptococcus (Pediococcus pentosaceus), the strain BG-6bl is Lactobacillus sakei, strain BY-Fa5, BY-Ff3, BY-Fe21, BY-Fe3, BG-8b2 is wesella eating sinus, Lactobacillus pentose Lactobacillus (Lactobacillus plantarum).
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：X172

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