铜和土霉素复合暴露对玉米幼苗生长发育的影响

发布时间：2018-06-04 08:48

本文选题：土霉素 + 铜　；参考：《环境与健康杂志》2016年12期

【摘要】：目的探讨铜和土霉素复合暴露对玉米幼苗生长发育的影响。方法观察铜(Cu~(2+))和土霉素(OTC)单一暴露及Cu~(2+)+OTC复合暴露对其发芽、总根长、叶片抗氧化酶和丙二醛含量的影响。结果 OTC、Cu~(2+)单一暴露、Cu~(2+)+OTC复合暴露对玉米种子发芽率的影响无统计学意义(P0.05)。与对照组相比,Cu~(2+)(20、30 mg/L)单一暴露组总根长在8 h显著减低,随着生长时间的延续,Cu~(2+)(20、30和40 mg/L)单一暴露组总根长在96 h明显减低,差异有统计学意义(P0.05);与对照组相比,Cu~(2+)+OTC(30、40 mg/L)组处理8 h玉米幼苗根系总根长缩短,随着玉米幼苗的生长,在24 h~96 h Cu~(2+)+OTC(10~40 mg/L)复合暴露组玉米幼苗根系总根长缩短,差异有统计学意义(P0.05)。Cu~(2+)+OTC复合暴露与Cu~(2+)、OTC单一暴露对玉米幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活力没有明显影响(P0.05)。与对照组相比,40 mg/L Cu~(2+)单一暴露组MDA含量的升高,差异有统计学意义(P0.05);而OTC单一暴露组玉米幼苗叶片中MDA含量无明显变化(P0.05);Cu~(2+)+OTC(20~40 mg/L)复合暴露组8~120 h MDA含量增加,差异有统计学意义(P0.05)。结论 Cu~(2+)单一暴露和Cu~(2+)+OTC复合暴露对玉米幼苗总根长有一定的抑制作用,且叶片组织发生氧化损伤。
[Abstract]:Objective to study the effects of copper and oxytetracycline combined exposure on the growth and development of maize seedlings. Methods the effects of single and Cu~(2 OTC exposure on the germination, total root length, antioxidant enzymes and malondialdehyde contents in leaves were observed. Results the effect of OTC compound exposure on germination rate of maize seeds was not statistically significant (P 0.05). Compared with the control group, the total root length of the single exposure group decreased significantly at 8 h, and the total root length of the single exposure group decreased significantly at 96 h with the growth time extending. Compared with the control group, the total root length of maize seedlings was shortened after 8 h treatment, and the total root length of maize seedlings was shortened at 24 h ~ 96 h (Cu~(2) OTC(10~40 mg / L) with the growth of maize seedlings, compared with the control group, the total root length of maize seedlings was shorter than that of the control group, and the total root length of maize seedlings was shortened at 24 h and 96 h after the treatment of OTCX 30 mg / L, and the total root length of maize seedlings decreased with the growth of maize seedlings. The difference was statistically significant (P 0.05) OTC combined exposure and Cu~(2 + OTC single exposure had no significant effect on the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (catalase) in the leaves of maize seedlings. Compared with the control group, the content of MDA in the single exposure group (40 mg/L Cu~(2) was higher than that in the control group, the difference was statistically significant (P 0.05), while the content of MDA in the leaves of maize seedlings in the OTC single exposure group had no significant change. The difference was statistically significant (P 0.05). Conclusion Cu~(2) single exposure and Cu~(2) OTC combined exposure can inhibit the total root length of maize seedlings and cause oxidative damage to leaf tissue.
【作者单位】：扬州大学环境科学与工程学院江苏省环境材料与环境工程重点实验室;浙江省植物进化生态学与保护重点实验室;
【基金】：国家自然科学基金(31500425) 江苏省自然科学基金青年科技人才项目(BK20150452) 扬州市自然科学基金青年科技人才项目(YZ2014053) 浙江省植物进化生态学与保护重点实验室开放课题(EEC2014-02)
【分类号】：X503.231;S513

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