双酚A对豚鼠的免疫毒性

发布时间：2018-06-07 10:56

本文选题：BPA + 免疫毒性　；参考：《环境科学研究》2016年09期

【摘要】：为了研究BPA(bisphenol A,双酚A)对豚鼠的免疫毒性,经口灌服,以不同剂量(500、1 000 mg/kg)染毒14 d后接种O型口蹄疫疫苗,通过分析脏器系数、淋巴细胞增殖能力、NK细胞(natural killer cell,自然杀伤细胞)活性、特异性抗体水平以及细胞免疫水平,评价BPA染毒剂量对豚鼠体液免疫和细胞免疫的影响.结果表明:与阴性对照组相比,BPA染毒后豚鼠体质量增加,心脏、肝脏、肾脏、脑、脾脏和卵巢体质量也增加,相应脏器系数升高;而胸腺、睾丸和附睾萎缩,相应脏器系数降低.NK细胞数量虽有增加,但变化不显著;BPA染毒引起NK细胞对靶细胞的杀伤能力显著下降,并且随着BPA染毒剂量的增加,其对靶细胞的杀伤能力显著下降;淋巴细胞刺激指数显著升高,并与染毒剂量呈正相关.BPA对豚鼠的特异性抗体水平有一定抑制作用,并且随着染毒剂量的增加该抑制作用愈发显著.IL-2(白介素2,interleukin-2)和IFN-γ(干扰素-γ,interferon)水平低于阴性对照组,而IL-4(白介素4,interleukin-4)水平高于阴性对照组;随着染毒剂量的增加,淋巴细胞增殖能力、IL-2和IFN-γ水平显著降低,而IL-4水平显著升高.研究显示,BPA会损伤豚鼠的免疫器官,抑制机体产生体液免疫和细胞免疫,导致免疫功能紊乱.
[Abstract]:In order to study the immune toxicity of BPA(bisphenol A, bisphenol A (BPA) in guinea pigs, oral administration of different doses of 500 000 mg / kg for 14 days was used to inoculate O type foot-and-mouth disease vaccine, and the organ coefficients were analyzed. The activity of NK cell natural killer cell (natural killer cell), the level of specific antibody and the level of cellular immunity were evaluated. The effects of BPA dose on humoral and cellular immunity of guinea pigs were evaluated. The results showed that compared with the negative control group, the body mass of the heart, liver, kidney, brain, spleen and ovary also increased, and the organ coefficients of the thymus, testis and epididymis were atrophied. The number of NK cells increased, but the cytotoxicity of NK cells to target cells decreased significantly with the increase of dose of BPA, and the cytotoxicity of NK cells to target cells decreased with the increase of dose of BPA. The lymphocyte stimulating index (LSI) was significantly increased, and there was a positive correlation with the dose of exposure. BPA inhibited the specific antibody level in guinea pigs to some extent. With the increase of dose, the inhibitory effect of IL-2and IFN- 纬 was significantly lower than that of negative control group, but the level of IL-4 was higher than that of negative control group, and with the increase of dose, the level of IL-4 was higher than that of negative control group, and the level of IL-4 was higher than that of negative control group. The levels of IL-2 and IFN- 纬 decreased significantly, while the level of IL-4 increased significantly. It has been shown that BPA can damage the immune organs of guinea pigs, inhibit the production of humoral and cellular immunity, and lead to the disorder of immune function.
【作者单位】：吉林建筑大学松辽流域水环境教育部重点实验室;军事医学科学院军事兽医研究所;
【基金】：吉林省教育厅“十三五”科学研究规划项目(20150168) 国家自然科学基金项目(51208226)
【分类号】：X171.5

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