内聚玉米芯固定化SRB污泥PRB系统修复煤矿酸性废水研究

发布时间：2018-06-09 00:35

本文选题：玉米芯 + 麦饭石　；参考：《辽宁工程技术大学》2015年硕士论文

【摘要】：近年来随着我国煤炭工业的快速发展,酸性矿井水污染日趋严重,矿区水资源供需的矛盾和生态环境的恶化已严重制约了煤炭工业的可持续性发展。因此,实现煤矿酸性废水(AMD)的清洁排放、循环利用具有重大意义。当前,以硫酸盐还原菌(SRB)为核心的新工艺已在有机废水、酸性废水和电镀废水等研究领域取得了较大进展。应用SRB技术处理AMD业已成为该领域发展的新趋势。本论文基于微生物固定化技术,采用碳源缓释原理,即在水解微生物作用下分解玉米芯的方法为SRB提供代谢碳源,并在固定化凝胶中加入铁屑和麦饭石,营造SRB适宜的微环境。通过正交试验确定固定化过程中的最优基质配比,考察交联化方法和交联时间对颗粒催化活性和操作稳定性的影响。通过厌氧批次试验研究玉米芯内聚量和AMD污染负荷率对颗粒代谢的影响,并分析颗粒处理能力以及活化颗粒对Mn~(2+)的响应变化。最后,以固定化颗粒为介质构建原位修复装置,讨论AMD原位修复的可行性和有效性。所得主要结论如下：(1)采用聚乙烯醇—硼酸包埋固定法,颗粒的最优基质配比为30%SRB污泥、2%铁屑、3%麦饭石。颗粒膨胀率、活性与交联时间分别呈线性负相关、一介衰减指数相关,从保持颗粒稳定性与活性角度出发可将交联时间设定为4-8h。(2)缓释碳源玉米芯水解引起有机物累积的平均速率为932mg/(L-d),最大累积量为478mgCOD/10g颗粒,固定化颗粒在pH值为2-6和Mn~(2+)浓度小于55mg/L的AMD中都具有良好的活性,可用伪二级动力学模型描述颗粒对Mn~(2+)的吸附过程(R2=0.995)。Mn~(2+)吸附曲线能同时较好的符合Langmuir (R2=0.983)和Frundlich (R2=0.996)模型,最大理论吸附量为0.998mg/g。(3)活化颗粒还原S042-的最大还原速率为94.88mg/(L·d),一级反应动力学模型能较好的描述还原过程(R2=0.962,k/=0.0997h)。高浓度Mn~(2+)能抑制颗粒提升pH值能力,在浓度梯度作用下Mn~(2+)扩散进入颗粒内,可直接降低SRB活性,减小S042-还原速率,同时也对水解微生物产生冲击。(4)UAPB与PRB均具有较强的pH提升能力,但是,UAPB出水OD600值增加显著,出水浑浊,玉米芯在UAPB中的水解受污染负荷变化影响较大,有机物的急剧累积和快速消耗降低了该系统对S042-处理的稳定性。PRB具有很慢的抗冲击能力,有机物累积存在稳定期,约1000mg/L,SO42的最大还原速率为1256mg/(L·d)。对TFe的去除率为99.9%以上,在固定化颗粒吸附位饱和之后,依据生化作用对Mn~(2+)的去除速率为3.74mg/(L·d)。以上研究表明,在固定化过程中将玉米芯内聚,采用污泥中混合菌落多样的代谢能力,较好的实现了碳源缓释与污染物去除的同步进行,以该固定化颗粒为填料的PRB系统可成为AMD原位处理的有效方法。
[Abstract]:In recent years, with the rapid development of coal industry in China, the water pollution in acid mines is becoming more and more serious. The contradiction between supply and demand of water resources and the deterioration of ecological environment in mining areas have seriously restricted the sustainable development of coal industry. Therefore, it is of great significance to realize clean discharge and recycling of acid coal mine wastewater. At present, the new process with SRB as the core has made great progress in the field of organic wastewater, acid wastewater and electroplating wastewater. Using SRB technology to deal with AMD has become a new trend in this field. Based on the technology of microorganism immobilization, this paper adopts the principle of slow release of carbon source, that is, the method of decomposing corn cob under the action of hydrolytic microorganism provides the metabolic carbon source for SRB, and adds iron chips and mai Fan stone into the immobilized gel to create a suitable microenvironment for SRB. The optimum ratio of substrate during immobilization was determined by orthogonal test. The effects of crosslinking method and crosslinking time on the catalytic activity and operational stability of particles were investigated. The effects of corncob cohesion and AMD pollution loading rate on particle metabolism were studied by anaerobic batch test. Finally, the feasibility and effectiveness of AMD in situ repair were discussed by using immobilized particles as the medium. The main conclusions are as follows: (1) Polyvinyl alcohol-boric acid entrapment was used. The optimum matrix ratio of the particles was 2% iron scraps of 30 SRB sludge and 3% mai fan stone. The particle expansion rate, activity and crosslinking time were linearly negatively correlated, and the first attenuation index was correlated. In order to maintain particle stability and activity, the average rate of organic matter accumulation induced by hydrolysis of sustained-release corn cob was 932 mg / L ~ (-1), and the maximum accumulation was 478 mg / 10 g corn cob, the crosslinking time was set at 4-8 h 路L ~ (-2). The immobilized particles have good activity in AMD with pH value of 2-6 and MnG-1 / L < 55mg / L. The pseudo-second-order kinetic model can be used to describe the adsorption process of Mn-Pu _ 2 by using pseudo-second-order kinetic model. The adsorption curves of the immobilized particles can be in good agreement with Langmuir R20.983) and Frundlich R2O _ (0.996) models at the same time, and the adsorption curves of the immobilized particles are in good agreement with the Langmuir R20.983) model and the Frundlich R2O _ (0.996) model. The maximum theoretical adsorption capacity is 0.998mg / g 路m3) the maximum reduction rate of activated particle S042- is 94.88 mg / L ~ (-1), and the first-order reaction kinetic model can better describe the reduction process (R _ (22) 0.962g / L ~ (-1) ~ (0.0997hg) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1). High concentration of MNO _ 2) can inhibit the ability of increasing pH value of particles. Under the action of concentration gradient, MNO _ 2) diffuses into the particles, which can directly reduce the activity of SRB and decrease the rate of S042-reduction. At the same time, both UAPB and PRB had strong pH lifting ability to hydrolyze microorganism, but the OD600 value of UAPB effluent increased significantly, the effluent was turbid, and the hydrolysis of corn cob in UAPB was greatly affected by the pollution load change. The rapid accumulation and rapid consumption of organic matter reduced the stability of S042- treatment. PRB had a slow impact resistance. The organic matter accumulation existed in a stable period, and the maximum reduction rate of about 1000mg / L ~ (-1) so _ (2) was 1256 mg / L / L ~ (-1) D ~ (-1) ~ (-1) ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1). The removal rate of TFE is more than 99.9%. After adsorption saturation of immobilized particles, the removal rate of Mn-Pb-2 is 3.74 mg / L 路d ~ (2 +) according to biochemical action. The results showed that, in the process of immobilization, the corn cob was cohesive and the mixed colony of sludge was used to metabolize the compost, and the slow release of carbon source and the removal of pollutants could be achieved synchronously. The PRB system with the immobilized particles as filler can be an effective method for AMD in situ treatment.
【学位授予单位】：辽宁工程技术大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X752

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