镉对果蝇金属硫蛋白表达的影响

发布时间：2018-06-16 21:05

本文选题：环境科学技术基础学科 + 镉　；参考：《安全与环境学报》2016年06期

【摘要】：以黑腹果蝇Canton-S品系为试验材料,采用镉饱和法测定重金属镉胁迫后果蝇金属硫蛋白质量比的变化,并应用实时荧光定量PCR(Real-Time PCR)方法检测了不同质量浓度镉处理后果蝇金属硫蛋白相关基因MtnB、MtnC、MtnD、MtnE及金属耐受因子Hmt-1基因mRNA水平的表达差异。结果表明,1)不同质量浓度镉处理对雌、雄果蝇均产生明显的生理影响,导致果蝇寿命缩短。镉胁迫果蝇10 d后,其半数致死质量浓度为52.0 mg/L,为后期果蝇对镉胁迫产生的解毒机制研究提供浓度设定依据。2)镉胁迫后雌、雄果蝇体内金属硫蛋白质量比显著升高,与对照组相比差异显著,并存在一定的剂量依赖性;3)镉胁迫导致黑腹果蝇金属硫蛋白相关基因mRNA水平表达上调,并具明显的剂量依赖性,同时在一定范围内Hmt-1基因的表达随镉质量浓度升高而上调,具有浓度依赖效应。
[Abstract]:Using Canton-S strain of Drosophila melanogaster as the experimental material, the changes of the ratio of metal sulfur to protein in Drosophila melanogaster after cadmium stress were determined by cadmium saturation method. The mRNA levels of metallothionein-associated gene (MtnBnC) and metal tolerance factor Hmt-1 (Hmt-1) in Drosophila melanogaster treated with cadmium were detected by real-time fluorescence quantitative PCR. The results showed that different concentrations of cadmium had obvious physiological effects on both female and male Drosophila melanogaster, which resulted in the shortening of life span of Drosophila melanogaster. After 10 days of cadmium stress, the LD50 of Drosophila melanogaster was 52.0 mg / L, which provided the basis of concentration setting for the study of mechanism of detoxification induced by CD stress in Drosophila melanogaster. 2) the ratio of metal sulfur to protein in male fruit fly increased significantly after cadmium stress. Compared with the control group, the expression of metallothionein-related gene mRNA in Drosophila melanogaster was up-regulated by cadmium stress in a dose-dependent manner, and there was a significant dose-dependent effect on the expression of metallothionein-related gene in Drosophila melanogaster. At the same time, Hmt-1 gene expression was up-regulated with the increase of cadmium concentration in a certain range, and had a concentration-dependent effect.
【作者单位】：陕西师范大学生命科学学院;
【基金】：国家自然科学基金项目(31201762) 中央高校基本科研业务费专项(GK201402026)
【分类号】：X171.5

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