厌氧颗粒污泥处理含2,4,6-三氯酚废水及其微生物种群结构的研究

发布时间：2018-06-17 22:40

本文选题：2 + 4　；参考：《湘潭大学》2015年硕士论文

【摘要】：由于CPs(氯酚类化合物)物质的大规模生产和应用,其排放量较大,已经对自然环境和人类健康构成了严重威胁。本研究以典型CPs物质2,4,6-三氯酚(2,4,6-TCP)为研究对象,从湖南岳阳造纸厂厌氧反应器中取得厌氧污泥,建立厌氧生物反应体系,培养驯化具有降解2,4,6-TCP能力的厌氧颗粒污泥,考察驯化前后污泥微生物种群结构的变化情况;在驯化成功后,采用间歇摇瓶试验和PCR-DGGE(聚合酶链式反应-变形梯度凝胶电泳)技术,考察外加碳源和金属对厌氧颗粒污泥降解2,4,6-TCP的效能及微生物种群结构的影响;并且进一步研究了葡萄糖浓度对厌氧颗粒污泥降解2,4,6-TCP的影响及其机理。研究得到了如下结论:(1)经过2,4,6-TCP驯化之后,厌氧颗粒污泥的微生物多样性减少,富集了6种2,4,6-TCP降解菌,分别为:Thermotog(热袍菌属)、Petrimonas sp.(理研菌属)、Escherichia(埃希氏菌属)、Bacillus(芽孢杆菌属)、Clostridium(梭状芽胞杆菌属)、Burkholderia(伯克霍尔德氏菌属);产生了新的2,4,6-TCP降解菌Shigella(志贺氏菌属)。(2)不同种类的外加金属对厌氧颗粒污泥降解2,4,6-三氯酚的效果影响差异较大,外加金属铁对其有促进作用,可促进Escherichia和Clostridium的生长;镍和锰有抑制作用,Ni可促进Acidobacterium(酸杆菌属)的生长,Mn可促进Mycobacterium(分支杆菌属)的生长,同时Ni和Mn对Escherichia和Clostridium的生长都有抑制作用。(3)2,4,6-TCP的厌氧降解过程是一个共代谢过程。外加碳源的存在能有效的提高厌氧颗粒污泥对2,4,6-TCP的降解速率,不同碳源对2,4,6-TCP的厌氧降解促进作用大小顺序依次为:葡萄糖乙酸钠丙酮酸钠酵母膏。(4)葡萄糖浓度对厌氧颗粒污泥降解2,4,6-TCP的种群结构有较大影响,当葡萄糖浓度为2g/L时,2,4,6-TCP降解菌(Enterobucteriaceae bacterium.、Escherichia sp.、Shigella、Comamonas sp.、Clostridium)在整个微生物体系中占优势地位,且2,4,6-TCP降解率达到最大值,但葡萄糖浓度太高(如10g/L时),利用葡萄糖、但不能降解2,4,6-TCP的菌(Bacteroides)大量繁殖,2,4,6-TCP降解率下降。(5)在“厌氧颗粒污泥-2g/L葡萄糖”体系中,2,4,6-TCP的降解途径为:2,4,6-TCP→2,4-DCP→4-CP→苯酚→矿化,“厌氧颗粒污泥-10g/L葡萄糖”体系中,2,4,6-TCP的降解路径为:2,4,6-TCP→2,4-DCP→4-CP。
[Abstract]:Because of the large-scale production and application of CPs (chlorophenol compounds), their emissions are large, which has posed a serious threat to the natural environment and human health. In this study, anaerobic sludge was obtained from the anaerobic reactor of Yueyang Paper Mill in Hunan Province, and the anaerobic granular sludge with the ability of degrading 2n46-TCP was cultivated and domesticated. After acclimation, batch shaking flask test and PCR-DGGE (Polymerase chain reaction-deformation gradient Gel Electrophoresis) were used to investigate the changes of microbial population before and after acclimation. The effects of added carbon and metals on the degradation of 2n46-TCP and the microbial population structure of anaerobic granular sludge were investigated, and the effect of glucose concentration on the degradation of 2O46-TCP by anaerobic granular sludge and its mechanism were further studied. The results showed that the microbial diversity of anaerobic granular sludge decreased after the acclimation of 2N 4N 6-TCP, and 6 species of 2C46-TCP biodegradable bacteria were enriched, which were: 1. Thermotog (Pyropaea sp. Petrimonas). (Escherichia coli) Bacillus (Bacillus sp.) Clostridium (Clostridium sp.) (Bacillus sp.); A new strain of Shilagelia (Shilagelia. 2) produced a new strain of Shilagelia (Shilagelia. 2). Different kinds of added metals degrade the anaerobic granular sludge of 2H4. 6 The effect of trichlorophenol was different. The addition of metal iron can promote the growth of Escherichia and Clostridium, and Ni and mn can inhibit the growth of Acidobacterium. Mn can promote the growth of Mycobacterium. At the same time, both Ni and mn inhibited the growth of Escherichia and Clostridium. The anaerobic degradation process of 46-TCP was a co-metabolic process. The existence of additional carbon source can effectively improve the degradation rate of 2N 46-TCP by anaerobic granular sludge. The order of promoting anaerobic degradation by different carbon sources was as follows: sodium glycolate sodium pyruvate yeast extract. 4) glucose concentration had a great effect on the population structure of anaerobic granular sludge degradation of 2H4 + 6-TCP. When glucose concentration is 2 g / L, the bacteria Enterobucteriaceae bacterium Escherichia sp. Shigellaus Comamonas sp. Clostridium is dominant in the whole microbial system, and the degradation rate of 2G / L is the highest, but the concentration of glucose is too high (for example, 10 g / L). However, Bacteroides, a bacterium that can not degrade 2G / 4G / L TCP, propagated in large numbers. The degradation rate of 2G / 4N / 6TCP was decreased. 5) in the anaerobic granular sludge-2g / L glucose system, the degradation pathway was: 1 / 2G / L / L glucose / 2g / L / L / g / L glucose / glucose system, respectively, and the pathway of degradation was as follows: 1 / 2N / 4 / 6-TCP / 24-DCP / 4-DCP / 4-CP phenol mineralization. In the anaerobic granular sludge -10g / L glucose system, the degradation path of 46-TCP is: 1 / 2G / L / L Glucose-10 g / L glucose.
【学位授予单位】：湘潭大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X703;X172

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