可降解三乙胺的赤红球菌S6-2的筛选与鉴定及降解特性

发布时间：2018-06-18 15:02

  本文选题：三乙胺 + 生物降解　； 参考：《环境科学研究》2016年12期

【摘要】：为治理制药废水中残留的难降解有机污染物TEA(triethylamine,三乙胺),以石家庄某污水处理厂的活性污泥为材料,采用富集培养和选择培养,分离筛选到1株能以TEA为唯一碳源和氮源生长代谢的降解菌——S6-2.通过测定形态特征、生理生化特性、G+C(碱基对)摩尔百分比及16S rRNA基因序列系统发育分析,该菌株被鉴定为赤红球菌(Rhodococcus ruber).采用单一影响因素试验分析菌株S6-2对TEA的降解特性,结果表明:菌株S6-2具有较强的TEA降解能力及降解稳定性;其最适降解温度为32℃,最适降解pH为8.0.菌株S6-2可耐受较高浓度TEA,在ρ(TEA)为900 mg/L的培养基中仍能生长;在最适条件下,菌株S6-2对TEA的降解率为70.7%±1.8%.该株菌在含TEA的无机盐平板上传代培养15代后,对TEA的降解率为69.3%±2.5%,说明菌株S6-2对TEA的降解具有稳定性.研究显示,菌株S6-2可作为TEA污染水体生物修复的潜在资源.
[Abstract]:In order to treat the residual refractory organic pollutants (tea triethylamine and triethylamine) in pharmaceutical wastewater, the activated sludge from a sewage treatment plant in Shijiazhuang was used as the material for enrichment and selective culture. A biodegradable strain, S6-2, was isolated and screened to use tea as the sole carbon source and nitrogen source for growth and metabolism. The strain was identified as Rhodococcus ruberus by determining the morphological characteristics, physiological and biochemical characteristics, G C (base pair) molar percentage and phylogenetic analysis of 16s rRNA gene sequence. A single factor test was used to analyze the degradation characteristics of tea by strain S6-2. The results showed that the strain S6-2 had strong degradation ability and stability of tea, the optimum degradation temperature was 32 鈩，

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