狼尾草根系对阿特拉津长期胁迫的氧化应激响应
发布时间:2018-06-18 18:08
本文选题:阿特拉津 + 狼尾草 ; 参考:《生态毒理学报》2016年06期
【摘要】:通过盆栽实验研究了抗性植物狼尾草根部丙二醛(MDA)、脯氨酸(Pro)、抗坏血酸(As A)含量及超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)等氧化应激生理指标对不同浓度阿特拉津长期(48 d)胁迫的响应规律。结果表明:当阿特拉津胁迫浓度分别高于20 mg·kg~(-1)和50 mg·kg~(-1)时,狼尾草根系的MDA与Pro含量较对照组显著升高(P0.05);随着阿特拉津胁迫浓度的增加,狼尾草根部SOD和GR活性呈先升高后降低的趋势,其中当阿特拉津胁迫浓度为20 mg·kg~(-1)时,SOD和GR活性达到最大值;供试植物根系中As A含量与阿特拉津胁迫浓度呈正相关。综上,中低浓度(≤20 mg·kg~(-1))阿特拉津处理没有对狼尾草的根系产生明显的氧化胁迫效应,狼尾草根系的上述抗氧化应激生理指标对于发挥阿特拉津抗性起着重要的作用。
[Abstract]:In pot experiment, the contents of malondialdehyde (MDAA), proline Pron, ascorbic acid (as A) in the roots of resistant plant, and the effects of oxidative stress physiological indexes, such as superoxide dismutase (SOD), glutathione reductase (Glutathione reductase), etc., on the length of atrazine at different concentrations were studied. The response to stress was observed at 48 d. The results showed that when atrazine concentration was higher than 20 mg kg 路kg -1 and 50 mg kg 路kg ~ (-1), MDA and Pro content in root system were significantly higher than that in control group, and with the increase of atrazine stress concentration. The activity of SOD and gr increased first and then decreased, and the activity of SOD and gr reached the maximum when atrazine concentration was 20 mg kg 路kg ~ (-1), and the content of as in the roots of the tested plants was positively correlated with the stress concentration of atrazine. In conclusion, the medium and low concentration (鈮,
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