甲醛和甲醇染毒对小鼠造血组织的毒性作用

发布时间：2018-06-22 06:54

本文选题：甲醛 + 甲醇　；参考：《环境科学研究》2016年12期

【摘要】：为了探究甲醛(Formaldehyde,FA)对造血组织的毒性,分析甲醛的毒性是否与中间代谢产物——甲醇(Methanol,MeOH)的毒性一致,以72只昆明小鼠为受试动物,随机分为6组〔1对照组,仅灌服生理盐水;2FA40组,单独灌服甲醛(FA),w(FA)为40 mg/kg;3 MeOH40组,单独灌服甲醇(MeOH),w(MeOH)为40 mg/kg;4FA_(10)+MeOH30组,同时灌服甲醛和甲醇,w(FA)为10mg/kg、w(MeOH)为30 mg/kg;5FA_(20)+MeOH20组,同时灌服甲醛和甲醇,w(FA)为20 mg/kg、w(MeOH)为20 mg/kg;6FA_(30)+MeOH10组〕,同时灌服甲醛和甲醇,w(FA)为30 mg/kg、w(MeOH)为10 mg/kg,连续灌胃7 d,检测小鼠的肝脏氧化损伤程度、血液和骨髓内甲醛含量以及甲醛脱氢酶的相对表达量,比较甲醇和甲醛毒性的差异性.结果表明:与对照组相比,各染毒组小鼠肝脏内ROS(reactive oxygen,活性氧)含量极显著增加(P0.01),FA_(30)+MeOH10组的ROS含量显著上升(P0.05);肝脏内MDA(malondialdehyde,丙二醛)含量极显著下降(P0.01),GSH(glutathione,谷胱甘肽)含量极显著升高(P0.01);在甲醛和甲醇联合染毒组中,随着甲醛含量的增加和甲醇含量的减少,氧化损伤程度呈上升趋势.AHMT(4-amino-3-hydrazine-5-mercapto-1,2,4-triazole)法测定结果显示,各染毒组小鼠血液和骨髓中甲醛含量与对照组相比均无显著差异;RT-PCR法测定结果显示,各染毒组小鼠中甲醛脱氢酶的相对表达量较对照组显著增加;FA40组与MeOH40组甲醛脱氢酶的相对表达量差异显著(P0.01);与FA_(30)+MeOH10组相比,FA_(10)+MeOH30组中甲醛脱氢酶的相对表达量极显著增加(P0.01),甲醛和甲醇联合染毒组中甲醛脱氢酶的相对表达量随着甲醛含量的增加和甲醇含量的减少呈上升趋势.研究显示,甲醛和甲醇的毒性不一致,甲醛对造血组织的毒性可能存在其他方式.
[Abstract]:In order to investigate the toxicity of formaldehyde (FA) to hematopoietic tissue, and analyze whether the toxicity of formaldehyde is consistent with that of Methanolol MeOH, 72 Kunming mice were randomly divided into 6 groups (control group). Normal saline 2FA40 group, formalin (FA) w (FA) 40 mg / kg 3MeOH40 group, methanol (MeOH) 4FAH30 group (MeOH) 40 mg / kg ~ (10) FAH _ (10) MeOH30, formaldehyde and methanol w (FA) 10 mg 路kg ~ (-1) F _ (20) MeOH _ (20) MeOH _ (20) MeOH20 group, formaldehyde and methanol w (FA) 20 mg / kg 路kg ~ (-1) w (MeOH) = 20 mg / kg 路kg ~ (6) FAF _ (30) MeOH _ (10). At the same time, the concentrations of formaldehyde and methanol were 30 mg / kg 路kg ~ (-1) (MeOH) = 10 mg / kg for 7 days. The degree of liver oxidative damage, the formaldehyde content in blood and bone marrow, and the relative expression of formaldehyde dehydrogenase in blood and bone marrow were measured, and the difference of toxicity between methanol and formaldehyde was compared. The results showed that: compared with the control group, The content of Ros (reactive oxygen, in the liver of each exposure group was significantly increased (P0.01) and the content of malondialdehyde (MDA) in the liver was significantly decreased (P0.01), and the content of glutathione was significantly increased (P0.01) in the combined group of formaldehyde and methanol (P0.01), while the content of Ros in the liver increased significantly (P0.01), and the content of malondialdehyde (malondialdehyde) decreased significantly (P0.01) in the combined group of formaldehyde and methanol (P0.01), while in the combined group of formaldehyde and methanol, the content of Ros in the liver increased significantly (P0.01), and the content of glutathione in the liver increased significantly. With the increase of formaldehyde content and the decrease of methanol content, the degree of oxidative damage showed an increasing trend. AHMT (4-amino-3-hydrazine-5-mercapto-1mercapto-1titriazole) method showed that there was no significant difference in formaldehyde content in blood and bone marrow of mice in each group compared with the control group. The relative expression of formaldehyde dehydrogenase in mice of each exposure group was significantly higher than that in control group (P0.01), and the relative expression of formaldehyde dehydrogenase in FA10 MeOH30 group was extremely significant compared with FA30 MeOH10 group (P0.01). The relative expression of formaldehyde dehydrogenase increased with the increase of formaldehyde content and the decrease of methanol content. Studies have shown that the toxicity of formaldehyde to hematopoietic tissue is not consistent with that of methanol, and that there may be other ways of toxicity of formaldehyde to hematopoietic tissue.
【作者单位】：华中师范大学生命科学学院;
【基金】：国家自然科学基金重点项目(51136002)
【分类号】：X171.5

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