枯草芽孢杆菌对铜绿微囊藻抑制效果的研究

发布时间：2018-07-09 15:20

本文选题：枯草芽孢杆菌 + 铜绿微囊藻　；参考：《中国环境科学》2015年06期

【摘要】：为探讨枯草芽孢杆菌(Bacillus subtilis)对铜绿微囊藻(Microcystis aeruginosa)的抑制效果,在实验室条件下,研究了枯草芽孢杆菌不同生长时期(延迟期、对数期、稳定期和衰亡期)无菌滤液对铜绿微囊藻生长的影响、枯草芽孢杆菌抑制铜绿微囊藻生长的作用方式以及无菌滤液影响下铜绿微囊藻丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和光合色素含量的变化.结果显示:枯草芽孢杆菌对数期、稳定期和衰亡期滤液抑藻效果明显好于延迟期,作用第8d,对铜绿微囊藻的去除率分别达到81.19%、91.41%、91.82%;4个处理组铜绿微囊藻的叶绿素a含量均显著低于对照组.添加稳定期滤液后,铜绿微囊藻MDA含量显著升高,SOD活性先升高后降低;在对光合色素的影响中,类胡萝卜素受到的影响不如叶绿素a显著.结果表明,枯草芽孢杆菌对铜绿微囊藻的抑制效果是通过分泌胞外物质实现的,且分泌物具有很强的热稳定性.推测该胞外分泌物能够破坏光合色素,影响光合作用,抑制藻细胞的生长;同时抑制SOD活性,使细胞膜脂过氧化程度不断加深,进而破坏藻细胞的完整性,表现出对藻很强的抑制效果.
[Abstract]:In order to study the inhibitory effect of Bacillus subtilis on microcystis aeruginosa, the growth stages of Bacillus subtilis were studied in laboratory. The effects of aseptic filtrate on the growth of microcystis aeruginosa, the way of Bacillus subtilis inhibiting the growth of microcystis aeruginosa and the malondialdehyde (MDA) content of microcystis aeruginosa under the influence of aseptic filtrate. Changes of superoxide dismutase (SOD) activity and photosynthetic pigment content. The results showed that the algal inhibition effect of filtrate in logarithmic, stable and decaying stage of Bacillus subtilis was better than that in delayed stage. On the 8th day, the removal rates of microcystis aeruginosa reached 81.191.41 and 91.82, respectively, and the chlorophyll a content in the four treatment groups was significantly lower than that in the control group. The MDA content of microcystis aeruginosa increased at first and then decreased after adding stable filtrate, and the effect of carotenoid on photosynthetic pigments was less significant than that on chlorophyll a. The results showed that the inhibition effect of Bacillus subtilis on microcystis aeruginosa was achieved by secreting extracellular substances and the secretion had strong thermal stability. It is speculated that the extracellular secretion can destroy photosynthetic pigment, affect photosynthesis, inhibit the growth of algal cells, inhibit SOD activity, make the degree of lipid peroxidation of cell membrane deepen, and then destroy the integrity of algal cells. It showed a strong inhibitory effect on algae.
【作者单位】：中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室;上海海洋大学水产与生命学院;
【基金】：“十二五”农村领域国家科技计划课题(2012BAD25B01)
【分类号】：X172;X52

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