一株穗花狐尾藻内生菌的分离鉴定及其溶磷特征研究

发布时间：2018-08-07 14:21

【摘要】：通过组织表面消毒、切面培养和共生培养的方法从健康的穗花狐尾藻植株体内分离获得了一株具有较强溶磷能力的内生菌,系统发育分析和VITEK 2系统鉴定结果表明,内生菌与沙雷氏菌的亲缘关系最近,因此,命名为Serratia sp.SP5.纯培养实验表明,菌株SP5通过产生酸性物质溶磷,在培养96 h后溶磷能力达到最大,溶解性磷酸根的浓度高达269.60 mg·L-1,培养液的p H值下降至4.32.氮源通过降低菌株SP5的产酸能力而间接影响了其对磷的释放能力,菌株SP5溶磷的氮源优先利用顺序为铵根离子硝酸根离子尿素.适宜菌株SP5生长的p H值范围为5~9,酸性条件下菌株的溶磷能力大于碱性条件,而在p H值为7时与对照相比菌株对磷的相对释放量最大.p H值达到9时,菌株无明显溶磷能力.共存高浓度的金属离子时菌株SP5的溶磷效果明显降低,影响程度的大小顺序为MnFeMg.菌株SP5溶磷的最佳碳氮比为35∶1,在相同碳氮比下,不同的碳氮量会明显影响溶磷效果.底泥模拟培养条件下,菌株SP5可通过释放底泥中的闭蓄态磷(O-P)和钙磷(Ca-P)而明显提高水相中的磷酸根离子浓度,与对照相比,底泥中这两种磷形态分别下降了15.21%和34.87%,而水相中溶解性磷酸根离子是对照的1.5~2.0倍.研究结果为研究富营养化水体中磷的生物地球化学过程提供了新的科学数据.
[Abstract]:An endophytic bacterium with strong phosphorus solubilizing ability was isolated from the healthy plant of Sagitophyta by the methods of tissue surface disinfection, sectioned culture and symbiotic culture. The phylogenetic analysis and VITEK 2 system identification showed that the endophytic bacteria had strong phosphorus solubilizing ability. Endophytic bacteria were closely related to Shareh, so they were named Serratia sp. SP5. The pure culture experiment showed that the phosphate solubilizing ability of the strain SP5 reached the maximum after 96 h culture by producing acid substance. The concentration of soluble phosphate was 269.60 mg / L, and the pH value of the culture medium decreased to 4.32. Nitrogen source indirectly affected the phosphorus release ability of strain SP5 by reducing its acid production ability. The priority order of nitrogen source utilization of strain SP5 was ammonium ion nitrate ion urea. The pH value of the suitable strain SP5 growth was 5 ~ 9. The phosphorus solubilizing ability of the strain under acidic condition was higher than that of the alkaline condition, but when pH value was 7, the relative release of phosphorus reached 9, the strain had no obvious ability to dissolve phosphorus. When the metal ions coexist with high concentration, the phosphorus solubilization effect of the strain SP5 is obviously reduced, and the order of influence is MnFeMg. The optimum C / N ratio of the strain SP5 was 35: 1. Under the same C / N ratio, different carbon and nitrogen content could significantly affect the phosphorus dissolution effect. Under the simulated culture condition of sediment, the concentration of phosphate ion in water phase could be increased obviously by releasing closed phosphorus (O-P) and calcium phosphorus (Ca-P) from sediment, compared with the control. The two phosphorus forms in the sediment decreased by 15.21% and 34.87%, respectively, while the dissolved phosphate ions in the water phase were 1.5% and 2.0 times of the control. The results provide new scientific data for studying the biogeochemical process of phosphorus in eutrophic water.
【作者单位】：吉林农业大学资源与环境学院;吉林省商品粮基地土壤资源可持续利用重点实验室;华中科技大学环境科学与工程学院;
【基金】：国家自然科学基金(No.51109089) 吉林省科技发展计划项目(No.20130206031NY)~~
【分类号】：X172;X52

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