群体感应淬灭菌的分离及其膜污染控制性能
发布时间：2018-08-19 18:48
【摘要】：通过淬灭细菌的群体感应系统来抑制生物膜形成、防止膜生物污染的方法近年来受到广泛关注.本实验从实际运行污水处理厂活性污泥中分离出5株具有群体感应淬灭功能的菌株,其中菌株HG10对信号分子N-乙酰高丝氨酸环内酯(C6-HSL)分解能力最强.经16S rRNA基因序列比对,初步鉴定为蜡样芽孢杆菌(Bacillus cereus).用海藻酸钠将菌株HG10进行包埋固定,以探究其在膜过滤系统中对膜污染防治的效果.结果表明,经过8 d培养,添加细菌包埋珠(SA-HG10)的实验组B中膜通量为181.29 L·(m~2·h)~(-1),未投加包埋珠的对照组A膜通量为110.64 L·(m~2·h)~(-1),B组膜通量比A组高出63.86%;对微滤膜片上生物膜中EPS含量测定表明,实验组B中EPS多糖和蛋白质含量较对照组A分别减少了29%和48%,疏水性蛋白质含量的大量减少是造成膜污染减弱的主要原因;膜表面胞外聚合物(EPS)总含量减少了43%,表明投放SA-HG10细菌包埋珠对过滤膜片上生物膜形成具有明显抑制作用,改善了膜过滤性能.
[Abstract]:The methods of inhibiting biofilm formation and preventing membrane biofilm fouling through colony sensing system of quenched bacteria have been paid more and more attention in recent years. In this experiment, five strains with colony induction quenching function were isolated from activated sludge of practical sewage treatment plant. Among them, strain HG10 had the strongest ability to decompose signal molecule N- acetyl homoserine cyclic lactone (C6-HSL). Bacillus cereus (Bacillus cereus). Was identified as Bacillus cereus by 16s rRNA gene sequence alignment. The strain HG10 was immobilized with sodium alginate to investigate its effect on membrane fouling in membrane filtration system. The results showed that after 8 days of culture, the membrane flux of experimental group B was 181.29 L (m ~ (2 h) ~ (-1), that of control group was 110.64 L (m ~ (2 h) ~ (-1) ~ (-1), the flux of group B was 63.86 higher than that of group A, and the content of EPS in biofilm was determined. The contents of EPS polysaccharides and proteins in experimental group B were decreased by 29% and 48%, respectively, compared with control group A. the decrease of hydrophobic protein content was the main reason for the decrease of membrane fouling. The total content of extracellular polymer (EPS) on the membrane surface was reduced by 43%, which indicated that the implantation of SA-HG10 bacterial entrapment beads could significantly inhibit the formation of biofilm on the membrane and improve the filtration performance of the membrane.
【作者单位】： 北京大学深圳研究生院环境与能源学院聚硅酸盐复合环保材料工程实验室;
【基金】：深圳市科技计划项目(JCYJ20150626110817181,JCYJ20150806112326712,CXZZ20151117141320317)
【分类号】：X703;X172
[Abstract]:The methods of inhibiting biofilm formation and preventing membrane biofilm fouling through colony sensing system of quenched bacteria have been paid more and more attention in recent years. In this experiment, five strains with colony induction quenching function were isolated from activated sludge of practical sewage treatment plant. Among them, strain HG10 had the strongest ability to decompose signal molecule N- acetyl homoserine cyclic lactone (C6-HSL). Bacillus cereus (Bacillus cereus). Was identified as Bacillus cereus by 16s rRNA gene sequence alignment. The strain HG10 was immobilized with sodium alginate to investigate its effect on membrane fouling in membrane filtration system. The results showed that after 8 days of culture, the membrane flux of experimental group B was 181.29 L (m ~ (2 h) ~ (-1), that of control group was 110.64 L (m ~ (2 h) ~ (-1) ~ (-1), the flux of group B was 63.86 higher than that of group A, and the content of EPS in biofilm was determined. The contents of EPS polysaccharides and proteins in experimental group B were decreased by 29% and 48%, respectively, compared with control group A. the decrease of hydrophobic protein content was the main reason for the decrease of membrane fouling. The total content of extracellular polymer (EPS) on the membrane surface was reduced by 43%, which indicated that the implantation of SA-HG10 bacterial entrapment beads could significantly inhibit the formation of biofilm on the membrane and improve the filtration performance of the membrane.
【作者单位】： 北京大学深圳研究生院环境与能源学院聚硅酸盐复合环保材料工程实验室;
【基金】：深圳市科技计划项目(JCYJ20150626110817181,JCYJ20150806112326712,CXZZ20151117141320317)
【分类号】：X703;X172
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1 钟增涛,郑会明,高轶静,王卉,崔中利,李顺鹏,朱军;细菌中的群体感应[J];生命的化学;2003年06期
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