穗花狐尾藻内生细菌的溶磷特征及其对宿主植物修复水体的作用
发布时间：2018-12-26 10:41
【摘要】：目前,水体富营养化是我国自然水体面临的最为严重的污染问题。为治理水体富营养化已采用过物理、化学等多种方法,但都存在一些弊端,当前生物修复水体富营养化的方法有较大优势,应用前景广阔,生物修复中的植物、微生物修复富营养化水体为新兴的较有效的治理方法。植物修复是近年来发展起来的一种效果好、耗能低、成本低廉的治理富营养化水体的新技术,但北方水体中植物生长周期比较短,且植物对水体及底泥中的磷吸收较少,为克服水生植物在修复水体富营养化过程中遇到的以上难题,从水生植物中分离出具有促生并溶磷作用的植物内生菌与水生植物联合作用治理水体富营养化将是具有广阔应用前景的生物修复技术。本研究利用从穗花狐尾藻植株中分离筛选出具有溶磷作用的四株植物内生细菌并通过溶磷能力比较实验筛选出具有较高应用价值的溶磷内生细菌W3,初步探讨了其生长最适培养条件和在不同培养条件下的溶磷特性,并在应用模拟实验中对内生溶磷菌株W3的促生潜力、对底泥中不同形态磷的释放作用及其对宿主植物穗花狐尾藻修复水体的促进作用做了初步研究。主要研究结果如下:从穗花狐尾藻中分离出若干株植物内生菌,并作溶磷实验初步筛选出四株具有溶磷作用的植物内生细菌,对四株内生细菌做几项溶磷特征比较实验,分别测定四株内生细菌在溶磷培养基中培养48h、96h、144h后上清液中正磷酸盐浓度,以溶磷效果较佳的菌株W3为例,三次采样时间测得正磷酸盐浓度分别为56.16、269.60、282.05mg/L(对照组分别为5.98、5.62、6.05mg/L),由于96h增加量213.44mg/L显著高于144h增加量12.45mg/L,结果表明96h达到较佳溶磷效果;研究不同磷酸盐(卵磷脂、磷酸铝、磷酸钙)对菌株溶磷效果的影响,结果表明四株菌株溶解磷酸钙效果最好,对照组与加菌实验组的正磷酸盐浓度分别为25.62、157.07、158.79、239.60、58.84mg/L,筛选出优势溶磷菌株W3;在不同碳氮源对菌株溶磷效果比较实验研究中得出四株内生细菌在以葡萄糖为碳源、以硫酸铵为氮源的培养基中溶磷效果最佳,W3溶磷能力最强,其正磷酸盐浓度为499.09mg/L。研究表明菌株W3适宜生长的pH值范围为5.0-9.0,改变无机磷基础培养基中碳氮比对菌株W3的溶磷效果有影响,菌株W3在培养基初始pH值为7.0时溶磷效果最佳,发酵液pH值的变化与磷酸钙的溶解明显相关,菌株W3吲哚乙酸和铁载体的产量较高,具有一定促进植物生长的潜力,并且对底泥中不同形态的磷有一定的释放作用。内生溶磷细菌W3具有促进底泥中磷的转化作用,并在促进宿主植物穗花狐尾藻生长的同时提高了宿主植物对水体的修复效果。
[Abstract]:At present, eutrophication is the most serious pollution problem in China. In order to control eutrophication of water body, many methods have been used, such as physics and chemistry, but there are some disadvantages. Microbial remediation of eutrophication water is a new and effective treatment method. Phytoremediation is a new technique to treat eutrophication water with good effect, low energy consumption and low cost in recent years. However, the plant growth period in the northern water body is relatively short, and the plant absorbs less phosphorus in the water body and sediment. In order to overcome the above problems encountered by aquatic plants in the process of remediation of eutrophication, It will be a promising bioremediation technique to isolate plant endophytes which can promote growth and dissolve phosphorus from aquatic plants and combine them with aquatic plants to control eutrophication of water body. In this study, four endophytic bacteria with phosphorus dissolving activity were isolated and screened from the plants of Cercospora pumila, and the endophytic bacteria W3 with high application value were screened by comparison of phosphorus solubilizing ability. The optimum conditions for growth and the characteristics of phosphorus solubilization under different culture conditions were discussed, and the potential to promote the growth of endophytic phosphorus soluble strain W3 was studied in simulated experiments. In this paper, the release of phosphorus from sediment and its promoting effect on the recovery of water body by the host plant, Phaeopsis sphaeracea, were preliminarily studied. The main results are as follows: a number of endophytic bacteria were isolated from Sargassum spp. Four endophytic bacteria with phosphorus dissolving activity were screened by phosphorus solubilization experiment, and four endophytic bacteria were compared with other endophytic bacteria. The concentration of orthophosphate in the supernatant of four endophytic bacteria cultured in phosphate solubilizing medium for 48h, 96h and 144h respectively was determined. The strain W3, which had better phosphorus dissolution effect, was taken as an example. The concentration of orthophosphate measured at three sampling times was 56.16269.60282.05mg/L (the control group was 5.98% 5.62 mg / L). The increase of 213.44mg/L in 96h was significantly higher than that in 144h (12.45mg / L). The results showed that the phosphorus solubilization effect was better at 96 h. The effects of different phosphates (lecithin, aluminum phosphate, calcium phosphate) on the phosphorus dissolution of the strain were studied. The results showed that the four strains had the best effect on the dissolution of calcium phosphate. The concentrations of orthophosphate in the control group and the experimental group were 25.62157.07158.79239.6058.84mg / L, respectively. The results showed that four endophytic bacteria with glucose as carbon source and ammonium sulfate as nitrogen source had the best phosphorus solubilization effect, and W3 had the strongest phosphorus solubilization ability in different carbon and nitrogen sources, and the results showed that four endophytic bacteria had the best phosphorus solubilizing effect in the medium with glucose as carbon source and ammonium sulfate as nitrogen source. Its orthophosphate concentration is 499.09 mg / L. The results showed that the pH value of strain W3 was 5.0-9.0. The effect of phosphorus solubilization of strain W3 was best when the initial pH value of the medium was 7.0, and the ratio of carbon to nitrogen in the inorganic phosphorus basic medium had an influence on the phosphorus solubilization of strain W3. The change of pH value in fermentation broth was obviously related to the dissolution of calcium phosphate. The yield of indoleacetic acid and iron carrier of strain W3 was higher, which had the potential to promote plant growth and release phosphorus of different forms in sediment. Endophytic phosphorus-soluble bacteria W3 can promote the transformation of phosphorus in sediment and promote the growth of host plant, and improve the effect of host plants on water remediation.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X52;X17
本文编号：2391977
[Abstract]:At present, eutrophication is the most serious pollution problem in China. In order to control eutrophication of water body, many methods have been used, such as physics and chemistry, but there are some disadvantages. Microbial remediation of eutrophication water is a new and effective treatment method. Phytoremediation is a new technique to treat eutrophication water with good effect, low energy consumption and low cost in recent years. However, the plant growth period in the northern water body is relatively short, and the plant absorbs less phosphorus in the water body and sediment. In order to overcome the above problems encountered by aquatic plants in the process of remediation of eutrophication, It will be a promising bioremediation technique to isolate plant endophytes which can promote growth and dissolve phosphorus from aquatic plants and combine them with aquatic plants to control eutrophication of water body. In this study, four endophytic bacteria with phosphorus dissolving activity were isolated and screened from the plants of Cercospora pumila, and the endophytic bacteria W3 with high application value were screened by comparison of phosphorus solubilizing ability. The optimum conditions for growth and the characteristics of phosphorus solubilization under different culture conditions were discussed, and the potential to promote the growth of endophytic phosphorus soluble strain W3 was studied in simulated experiments. In this paper, the release of phosphorus from sediment and its promoting effect on the recovery of water body by the host plant, Phaeopsis sphaeracea, were preliminarily studied. The main results are as follows: a number of endophytic bacteria were isolated from Sargassum spp. Four endophytic bacteria with phosphorus dissolving activity were screened by phosphorus solubilization experiment, and four endophytic bacteria were compared with other endophytic bacteria. The concentration of orthophosphate in the supernatant of four endophytic bacteria cultured in phosphate solubilizing medium for 48h, 96h and 144h respectively was determined. The strain W3, which had better phosphorus dissolution effect, was taken as an example. The concentration of orthophosphate measured at three sampling times was 56.16269.60282.05mg/L (the control group was 5.98% 5.62 mg / L). The increase of 213.44mg/L in 96h was significantly higher than that in 144h (12.45mg / L). The results showed that the phosphorus solubilization effect was better at 96 h. The effects of different phosphates (lecithin, aluminum phosphate, calcium phosphate) on the phosphorus dissolution of the strain were studied. The results showed that the four strains had the best effect on the dissolution of calcium phosphate. The concentrations of orthophosphate in the control group and the experimental group were 25.62157.07158.79239.6058.84mg / L, respectively. The results showed that four endophytic bacteria with glucose as carbon source and ammonium sulfate as nitrogen source had the best phosphorus solubilization effect, and W3 had the strongest phosphorus solubilization ability in different carbon and nitrogen sources, and the results showed that four endophytic bacteria had the best phosphorus solubilizing effect in the medium with glucose as carbon source and ammonium sulfate as nitrogen source. Its orthophosphate concentration is 499.09 mg / L. The results showed that the pH value of strain W3 was 5.0-9.0. The effect of phosphorus solubilization of strain W3 was best when the initial pH value of the medium was 7.0, and the ratio of carbon to nitrogen in the inorganic phosphorus basic medium had an influence on the phosphorus solubilization of strain W3. The change of pH value in fermentation broth was obviously related to the dissolution of calcium phosphate. The yield of indoleacetic acid and iron carrier of strain W3 was higher, which had the potential to promote plant growth and release phosphorus of different forms in sediment. Endophytic phosphorus-soluble bacteria W3 can promote the transformation of phosphorus in sediment and promote the growth of host plant, and improve the effect of host plants on water remediation.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X52;X17
【参考文献】
相关期刊论文 前4条
1 吕娟;陈银广;顾国维;;好氧颗粒污泥在生物强化除磷中的应用[J];环境科学与技术;2006年07期
2 黄晓淳;骆和东;黄培枝;洪华荣;;我国水源水及饮用水中微囊藻毒素的污染现状及影响因素研究[J];环境卫生学杂志;2014年05期
3 王光华,周克琴,金剑,潘相文,赵英;不同碳源对三种溶磷真菌溶解磷矿粉能力的影响[J];生态学杂志;2004年02期
4 赵小蓉,林启美,李保国;溶磷菌对4种难溶性磷酸盐溶解能力的初步研究[J];微生物学报;2002年02期
相关博士学位论文 前1条
1 孙凤;饮用水处理工艺中微囊藻毒素污染调控技术的优化研究[D];山东大学;2013年
相关硕士学位论文 前2条
1 童昌华;水体富营养化发生原因分析及植物修复机理的研究[D];浙江大学;2004年
2 伊洋;富营养化水体净化高效生物菌剂的构建与效能研究[D];哈尔滨工业大学;2009年
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