微电流电解对藻类生长的抑制机理研究
发布时间：2019-02-12 22:35
【摘要】：保护湖泊、治理蓝藻水华是当今国内外的重要课题。已有研究表明,微电流电解法作为一种电化学处理技术,可利用电极的直接氧化以及电解产生的活性物质的间接氧化作用有效抑制水体中藻类的生长,并且对水中其它组分影响小,不会造成二次污染。本文以铜绿微囊藻为代表性藻类,采用微电流电解技术对藻类的生长进行有效抑制,探究了微电流电解有效抑藻的最优条件,研究了微电流电解对藻类生理生态特征的影响和电解间接氧化作用的抑藻效能,从多角度阐释了微电流电解抑藻的作用机理。主要研究结论如下:(1)比较了不同电极材料组合下微电流电解抑藻效果的差异,发现阳极材料对电解抑藻效率的影响较大,阴极材料对抑藻效率影响较小,选择了钌钛和不锈钢分别作为阳极和阴极材料。探究了微电流电解抑藻的主要影响因素,发现在一定范围内,电流密度、电解时间以及CaCl2浓度的增加均有助于提高电解抑藻效率,而电解液中Na2CO3以及MgSO4浓度的升高则会在一定程度上降低电解抑藻效率。采用BG-11培养基配制体积100ml、初始浓度为5×105个/mL的铜绿微囊藻液,以钌钛、不锈钢分别作阳阴极,以10mA/cm2的电流密度电解处理15 min即可实现对藻类生长的持续抑制。(2)通过研究铜绿微囊藻在不同电流密度下叶绿素荧光参数的变化,从生态生理特征角度揭示电解抑藻的作用机理。研究发现对于其他条件一定的藻液来说,抑藻存在相应的临界电流阈值。当电流密度小于临界值时,电解对藻细胞的胁迫未超过藻的耐受能力,藻液可在后期培养中恢复光合活性。若电流密度大于临界值,电解对藻的胁迫会超过藻的耐受能力,电解会破坏掉光合系统II的结构并阻断其与藻胆体之间的连接,使其无法进行光合作用,最终导致藻细胞的死亡。(3)从电解的间接氧化作用角度出发,验证了活性物质的抑藻能力,发现微电流电解过程中确实产生了具有抑藻效果的活性物质,足量的活性物质对铜绿微囊藻具有良好的抑制效果,可导致已受电解破坏的藻细胞不能进行自身修复而彻底死亡。对不同条件下间接氧化作用的抑藻能力进行了探究,研究发现在一定范围内,Cl-浓度、电流密度以及电解时间的增加均有助于提高微电流电解间接氧化的抑藻效果。(4)检测并比较了电解产生的活性物质中活性氯与过氧化氢的生成量,发现在不同电流密度、电解时间或Cl-浓度下,活性物质抑藻效率与活性氯生成量呈正相关,而与过氧化氢生成量的相关性不强。进一步对比有无各类活性物质作用时藻类的生长状况,得知活性氯与过氧化氢都具有一定的抑藻能力,但活性氯的抑藻能力高于过氧化氢,在活性物质抑藻过程中起主导作用。
[Abstract]:Protecting lakes and controlling cyanobacteria Shui Hua is an important subject at home and abroad. It has been shown that as a kind of electrochemical treatment technology, micro-current electrolysis can effectively inhibit algae growth by direct oxidation of electrodes and indirect oxidation of active substances produced by electrolysis. And it has little influence on other components in water, so it will not cause secondary pollution. In this paper, microcystis aeruginosa was used as the representative algae, and the microcurrent electrolysis technique was used to effectively inhibit the growth of algae. The effects of microcurrent electrolysis on the physiological and ecological characteristics of algae and the inhibition effect of indirect electrolytic oxidation on algae were studied. The mechanism of microcurrent electrolysis on algae inhibition was explained from many angles. The main conclusions are as follows: (1) the effect of microcurrent electrolysis on algae inhibition under different electrode combinations was compared. It was found that anode material had a great effect on the inhibition efficiency of algae electrolysis, and cathode material had little effect on algae inhibition efficiency. Ruthenium titanium and stainless steel were used as anode and cathode material respectively. It was found that the increase of current density, electrolysis time and CaCl2 concentration could improve the efficiency of algae inhibition. However, the increase of Na2CO3 and MgSO4 concentration in electrolyte will reduce the efficiency of algae inhibition to some extent. The solution of microcystis aeruginosa with initial concentration of 5 脳 105 / mL was prepared by using BG-11 medium. Ruthenium titanium and stainless steel were used as positive cathodes, respectively. Continuous inhibition of algae growth could be achieved by electrolytic treatment of 10mA/cm2 current density for 15 min. (2) the changes of chlorophyll fluorescence parameters of microcystis aeruginosa at different current densities were studied. The mechanism of electrolytic algal inhibition was revealed from the point of view of ecological physiological characteristics. It is found that there is a critical current threshold for algae inhibition for other algae under certain conditions. When the current density was less than the critical value, the stress of electrolysis to the algal cells did not exceed the tolerance ability of the algae, and the algae solution could recover the photosynthetic activity in the later culture. If the current density is greater than the critical value, electrolysis of algae will exceed the tolerance of algae, and electrolysis will destroy the structure of photosynthetic system II and block its connection with phycobilis, which makes it impossible to photosynthesis. Finally, the algal cells died. (3) from the point of view of indirect oxidation of electrolysis, the anti-algal ability of the active substances was verified, and it was found that the active substances with anti-algal effect were produced in the process of micro-current electrolysis. A sufficient amount of active substances can inhibit microcystis aeruginosa well, which can cause the cells destroyed by electrolysis to be unable to repair themselves and die completely. The inhibitory effect of indirect oxidation on algae under different conditions was investigated. It was found that the concentration of Cl- was within a certain range. The increase of current density and electrolysis time can improve the inhibition effect of microcurrent electrolysis indirect oxidation. (4) the amount of active chlorine and hydrogen peroxide produced by electrolysis is detected and compared. Under electrolysis time or Cl- concentration, the inhibitory efficiency of active substances was positively correlated with the amount of active chlorine, but not with the amount of hydrogen peroxide. Compared with the growth of algae with or without various active substances, it was found that both active chlorine and hydrogen peroxide had a certain ability to inhibit algae, but the inhibitory ability of active chlorine was higher than that of hydrogen peroxide, which played a leading role in the process of algal inhibition of active substances.
【学位授予单位】：长江科学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X52
本文编号：2420860
[Abstract]:Protecting lakes and controlling cyanobacteria Shui Hua is an important subject at home and abroad. It has been shown that as a kind of electrochemical treatment technology, micro-current electrolysis can effectively inhibit algae growth by direct oxidation of electrodes and indirect oxidation of active substances produced by electrolysis. And it has little influence on other components in water, so it will not cause secondary pollution. In this paper, microcystis aeruginosa was used as the representative algae, and the microcurrent electrolysis technique was used to effectively inhibit the growth of algae. The effects of microcurrent electrolysis on the physiological and ecological characteristics of algae and the inhibition effect of indirect electrolytic oxidation on algae were studied. The mechanism of microcurrent electrolysis on algae inhibition was explained from many angles. The main conclusions are as follows: (1) the effect of microcurrent electrolysis on algae inhibition under different electrode combinations was compared. It was found that anode material had a great effect on the inhibition efficiency of algae electrolysis, and cathode material had little effect on algae inhibition efficiency. Ruthenium titanium and stainless steel were used as anode and cathode material respectively. It was found that the increase of current density, electrolysis time and CaCl2 concentration could improve the efficiency of algae inhibition. However, the increase of Na2CO3 and MgSO4 concentration in electrolyte will reduce the efficiency of algae inhibition to some extent. The solution of microcystis aeruginosa with initial concentration of 5 脳 105 / mL was prepared by using BG-11 medium. Ruthenium titanium and stainless steel were used as positive cathodes, respectively. Continuous inhibition of algae growth could be achieved by electrolytic treatment of 10mA/cm2 current density for 15 min. (2) the changes of chlorophyll fluorescence parameters of microcystis aeruginosa at different current densities were studied. The mechanism of electrolytic algal inhibition was revealed from the point of view of ecological physiological characteristics. It is found that there is a critical current threshold for algae inhibition for other algae under certain conditions. When the current density was less than the critical value, the stress of electrolysis to the algal cells did not exceed the tolerance ability of the algae, and the algae solution could recover the photosynthetic activity in the later culture. If the current density is greater than the critical value, electrolysis of algae will exceed the tolerance of algae, and electrolysis will destroy the structure of photosynthetic system II and block its connection with phycobilis, which makes it impossible to photosynthesis. Finally, the algal cells died. (3) from the point of view of indirect oxidation of electrolysis, the anti-algal ability of the active substances was verified, and it was found that the active substances with anti-algal effect were produced in the process of micro-current electrolysis. A sufficient amount of active substances can inhibit microcystis aeruginosa well, which can cause the cells destroyed by electrolysis to be unable to repair themselves and die completely. The inhibitory effect of indirect oxidation on algae under different conditions was investigated. It was found that the concentration of Cl- was within a certain range. The increase of current density and electrolysis time can improve the inhibition effect of microcurrent electrolysis indirect oxidation. (4) the amount of active chlorine and hydrogen peroxide produced by electrolysis is detected and compared. Under electrolysis time or Cl- concentration, the inhibitory efficiency of active substances was positively correlated with the amount of active chlorine, but not with the amount of hydrogen peroxide. Compared with the growth of algae with or without various active substances, it was found that both active chlorine and hydrogen peroxide had a certain ability to inhibit algae, but the inhibitory ability of active chlorine was higher than that of hydrogen peroxide, which played a leading role in the process of algal inhibition of active substances.
【学位授予单位】：长江科学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X52
【参考文献】
相关期刊论文 前6条
1 许海;刘兆普;袁兰;杨林章;;pH对几种淡水藻类生长的影响[J];环境科学与技术;2009年01期
2 吴星五,唐秀华,朱爱莲,高廷耀;电化学杀藻水处理实验[J];工业水处理;2002年08期
3 蔡卓平;段舜山;朱红惠;;光密度法与计数法测定3种能源微藻细胞生长的相关性及其验证(英文)[J];南方农业学报;2012年10期
4 赵章元;我国江河湖海除藻的治标与治本浅析[J];环境保护;2000年08期
5 梁英;冯力霞;尹翠玲;曹春晖;;叶绿素荧光技术在微藻环境胁迫研究中的应用现状及前景[J];海洋科学;2007年01期
6 胡德荣;杜进富;汪艺朋;;蓝藻的危害与治理[J];首都师范大学学报(自然科学版);2007年04期
，本文编号：2420860
本文链接：https://www.wllwen.com/kejilunwen/huanjinggongchenglunwen/2420860.html
最近更新
教材专著
