草甘膦与杀虫单对不同倍性泥鳅鳍细胞系的毒性效应
发布时间：2019-03-18 17:04
【摘要】：本文研究了不同浓度草甘膦与杀虫单对60-70代泥鳅鳍二倍体细胞系(DIMF)和泥鳅鳍三倍体细胞系(TRMF)的毒性作用。采用噻唑蓝(MTT)法测得草甘膦对DIMF与TRMF 24h半致死浓度分别为333.79mg/L,384.91mg/L,杀虫单对DIMF与TRMF 24h半致死浓度分别为119.73mg/L,146.26mg/L。DIMF对草甘膦和杀虫单的敏感性明显高于TRMF。经草甘膦与杀虫单处理的活体细胞均表现为细胞拉长,空泡化和脱落并游离于培养基表面的现象。酶活力测定两种细胞系的结果显示:草甘膦浓度为0~300mg/L时,超氧化物歧化酶(SOD)和谷胱甘肽S转移酶(GST)活力随着浓度的增加而增加,随后逐渐降低;乙酰胆碱酯酶(ACh E)活力与草甘膦浓度呈负相关。杀虫单浓度为0~100mg/L时,SOD和GST活力随着浓度的增加而增加,100~200mg/L浓度组酶活力逐渐降低;ACh E活力与杀虫单浓度呈负相关。并且三种酶活力三倍体均高于二倍体。微核试验结果显示:100mg/L草甘膦与50mg/L杀虫单即能使细胞核产生损伤而形成微核,微核率随着草甘膦与杀虫单浓度增加而增加,草甘膦浓度为500mg/L和杀虫单浓度为200mg/L时微核率最大,草甘膦处理组DIMF与TRMF微核率均为4.3‰,杀虫单处理组DIMF与TRMF微核率分别为3.3‰和3.7‰。电镜观察结果是对照组DIMF和TRMF超微结构无明显差异。经草甘膦与杀虫单处理后,DIMF和TRMF病理变化情况相似:染色质凝集趋边,细胞核分解成多个;线粒体、核糖体与内质网等细胞器数量减少,细胞内出现空泡,出现凋亡小体,表现出凋亡的特征。本文不仅研究了草甘膦与杀虫单毒性的作用机制和不同倍性细胞系之间的差异,而且为建立草甘膦与杀虫单农药体外监控体系打下基础。
[Abstract]:In this paper, the toxicity of glyphosate and diploid of 60 渭 m 70 generation Loach fin diploid cell line (DIMF) and Loach fin triploid cell line (TRMF) was studied in different concentrations of glyphosate and insecticida. The 24-hour lethal concentrations of glyphosate to DIMF and TRMF were 333.79 mg / L, 384.91 mg / L and 119.73 mg / L for DIMF and TRMF, respectively, determined by thiazole blue (MTT) method, and the 24-hour lethal concentrations of glyphosate to DIMF and TRMF were 333.79 mg / L, 384.91 mg / L and 119.73 mg / L, respectively. The sensitivity of 146.26mg/L.DIMF to glyphosate and insecticidal list was significantly higher than that of TRMF.. The cells treated with glyphosate and insecticidal single showed the phenomenon of cell lengthening, vacuolization and exfoliation and dissociating on the surface of the culture medium. The results of enzyme activity assay showed that when the concentration of glyphosate was 0~300mg/L, the activities of superoxide dismutase (SOD) and glutathione S transferase (GST) increased with the increase of the concentration, and then decreased gradually. Acetylcholinesterase (ACh E) activity was negatively correlated with glyphosate concentration. When the concentration of 0~100mg/L was 0~100mg/L, the activities of SOD and GST increased with the increase of the concentration, and the decrease of the activity of; ACh E in the 100~200mg/L concentration group was negatively correlated with the concentration of the insecticidal single. The activity of the three enzymes in triploid was higher than that in diploid. The results of micronucleus test showed that 100mg/L glyphosate and 50mg/L were able to damage the nucleus and form micronucleus, and the micronucleus rate increased with the increase of glyphosate and insecticidal single concentration. When the concentrations of glyphosate were 500mg/L and 200mg/L, the micronucleus rates of DIMF and TRMF were 4.3 鈥，
本文编号：2443045
[Abstract]:In this paper, the toxicity of glyphosate and diploid of 60 渭 m 70 generation Loach fin diploid cell line (DIMF) and Loach fin triploid cell line (TRMF) was studied in different concentrations of glyphosate and insecticida. The 24-hour lethal concentrations of glyphosate to DIMF and TRMF were 333.79 mg / L, 384.91 mg / L and 119.73 mg / L for DIMF and TRMF, respectively, determined by thiazole blue (MTT) method, and the 24-hour lethal concentrations of glyphosate to DIMF and TRMF were 333.79 mg / L, 384.91 mg / L and 119.73 mg / L, respectively. The sensitivity of 146.26mg/L.DIMF to glyphosate and insecticidal list was significantly higher than that of TRMF.. The cells treated with glyphosate and insecticidal single showed the phenomenon of cell lengthening, vacuolization and exfoliation and dissociating on the surface of the culture medium. The results of enzyme activity assay showed that when the concentration of glyphosate was 0~300mg/L, the activities of superoxide dismutase (SOD) and glutathione S transferase (GST) increased with the increase of the concentration, and then decreased gradually. Acetylcholinesterase (ACh E) activity was negatively correlated with glyphosate concentration. When the concentration of 0~100mg/L was 0~100mg/L, the activities of SOD and GST increased with the increase of the concentration, and the decrease of the activity of; ACh E in the 100~200mg/L concentration group was negatively correlated with the concentration of the insecticidal single. The activity of the three enzymes in triploid was higher than that in diploid. The results of micronucleus test showed that 100mg/L glyphosate and 50mg/L were able to damage the nucleus and form micronucleus, and the micronucleus rate increased with the increase of glyphosate and insecticidal single concentration. When the concentrations of glyphosate were 500mg/L and 200mg/L, the micronucleus rates of DIMF and TRMF were 4.3 鈥，
本文编号：2443045
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