双信号细胞电化学法检测氯酚类的毒性

发布时间：2019-05-21 23:46

【摘要】：以多壁碳纳米管和离子液体复合修饰电极(MWCNTs-IL/GCE)为工作电极,运用线性扫描伏安法(LSV)研究了人乳腺癌(MCF-7)细胞的电化学行为,检测到了2个明显的氧化峰,其氧化峰电位分别为0.725 V和1.038 V.结合4种嘌呤碱基与模型细胞的电化学行为比较与高效液相色谱分析,确定细胞的电化学响应来源分别为细胞质中黄嘌呤与鸟嘌呤、次黄嘌呤与腺嘌呤的电化学氧化.基于2个响应信号峰电流的变化,研究了五氯酚(PCP)、2,4,6-三氯酚(TCP)和2,4-二氯酚(DCP)对MCF-7细胞的毒性效应,根据剂量-效应关系曲线计算半数抑制效应浓度(IC50)值,并与四甲基偶氮唑盐(MTT)比色法进行比较.结果发现,氯酚类污染物对MCF-7细胞活性有明显抑制作用,细胞电化学法与MTT法测得细胞毒性顺序均为:PCPTCPDCP,表明细胞电化学法可以快速、准确地评价氯酚类污染物的细胞毒性.该研究结果可从核苷酸代谢角度为危害化学品的安全无标记、准确客观的毒性评价方法建立提供新思路.
[Abstract]:The electrochemical behavior of human breast cancer (MCF-7) cells was studied by linear scanning Voltammetric method (LSV) using multi-wall carbon nanotube (CNT) and ionic liquid composite modified electrode (MWCNTs-IL/GCE) as working electrodes. Two obvious oxidation peaks were detected. The oxidation peak potentials are 0.725 V and 1.038 V, respectively. Combined with the comparison of electrochemical behavior of four purine bases and the analysis of high performance liquid chromatography (HPLC), the electrochemical response sources of the cells were determined to be the electrochemical oxidation of xanthine and guanine, Hypoxanthine and adenine in cytoplasm, respectively. Based on the changes of two response signal peak currents, the toxic effects of pentachlorophenol (PCP), 2, 4, 6-trichlorophenol (TCP) and 2, 4-dichlorophenol (DCP) on MCF-7 cells were studied. According to the dose-effect relationship curve, the 50% inhibitory effect concentration (IC50) was calculated and compared with that of tetramethylazole salt (MTT) colorimetric method. The results showed that chlorophenol pollutants had obvious inhibitory effect on the activity of MCF-7 cells. PCPTCPDCP, showed that the cytotoxicity order was as follows: PCPTCPDCP, showed that the cellular electrochemical method could be rapid. The cytotoxicity of chlorophenol pollutants was evaluated accurately. The results of this study can provide a new idea for the establishment of unmarked, accurate and objective toxicity evaluation methods for hazardous chemicals from the point of view of nucleotides metabolism.
【作者单位】：渤海大学化学化工学院;渤海大学工学院;
【基金】：国家自然科学基金(No.41602351,61401043,51309013) 辽宁省博士科研启动基金(No.201601344)~~
【分类号】：X171.5

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